Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P17_F_D04
(768 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 2e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 43 4e-05
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 38 0.001
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 36 0.006
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 35 0.011
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 33 0.045
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 32 0.078
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 30 0.42
SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|... 26 6.8
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M... 26 6.8
SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2 |Schizo... 25 9.0
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 9.0
SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subuni... 25 9.0
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 57.2 bits (132), Expect = 2e-09
Identities = 42/132 (31%), Positives = 67/132 (50%), Gaps = 8/132 (6%)
Frame = +2
Query: 380 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 556
+ ++ F D+DQ G ITS EL ++ + GQ+ + MI D D NG I+ EF
Sbjct: 13 EFREAFSLFDRDQDGNITSNEL-GVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFL 71
Query: 557 ----DKLYTYVN--QWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSN 718
K+ N + FK +D D +G+I EL+ LT +G R S E + + + ++
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREAD 131
Query: 719 TN-DGKISVDXF 751
T+ DG I+ + F
Sbjct: 132 TDGDGVINYEEF 143
Score = 40.3 bits (90), Expect = 3e-04
Identities = 22/62 (35%), Positives = 35/62 (56%)
Frame = +2
Query: 380 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 559
+V++ F+ DKD +G+IT EL L + G+ S+ MI D D +G IN +EF
Sbjct: 86 EVREAFKVFDKDGNGYITVEELTHVLTSL-GERLSQEEVADMIREADTDGDGVINYEEFS 144
Query: 560 KL 565
++
Sbjct: 145 RV 146
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 43.2 bits (97), Expect = 4e-05
Identities = 34/128 (26%), Positives = 57/128 (44%), Gaps = 3/128 (2%)
Frame = +2
Query: 380 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEFD 559
++++ F +DKD G I ++++ L + Q SE + N M + N L
Sbjct: 49 ELKEAFALLDKDGDGNIGREDVKTMLTSLN-QDASEDSINHMFESINPPINLAAFLTAMG 107
Query: 560 KLYTYV---NQWLAVFKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDG 730
+ + N L F T+D QSG I + AL+ MG R P+ + + RS T+ G
Sbjct: 108 SMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESIL-RSYTSHG 166
Query: 731 KISVDXFI 754
+ F+
Sbjct: 167 VFYYEKFV 174
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 38.3 bits (85), Expect = 0.001
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +2
Query: 596 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKISVDXFIVM 760
F+ +D D +G I GEL LT +G + S E ++ L K DG ++ F+ M
Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDFVQM 137
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/54 (33%), Positives = 27/54 (50%)
Frame = +2
Query: 395 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 556
F+ DKD +G I ELR L + + +E L+ G+ KD G +N +F
Sbjct: 83 FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKD--GMVNYHDF 134
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 35.9 bits (79), Expect = 0.006
Identities = 35/137 (25%), Positives = 58/137 (42%), Gaps = 13/137 (9%)
Frame = +2
Query: 380 QVQQWFRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF- 556
++++ F +D +QSG I E S A S + + + D+D G ++ EF
Sbjct: 25 RIRKRFIKIDANQSGSIDRNEFLSIPSVA-----SNPLASRLFSVVDEDGGGDVDFQEFI 79
Query: 557 DKLYTYV------NQWLAVFKTYDTDQSGFIDEGELSKALTQM-GFRFSPEFINILTKRS 715
+ L + + FK YD D+ G+I GEL L M G + + + ++
Sbjct: 80 NSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKT 139
Query: 716 -----NTNDGKISVDXF 751
DGKIS + F
Sbjct: 140 IMEVDKDRDGKISFEEF 156
Score = 31.5 bits (68), Expect = 0.14
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 4/66 (6%)
Frame = +2
Query: 395 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGM----FDKDRNGQINLDEFDK 562
F+ D D+ G+I++ EL L G E ++ DKDR+G+I+ +EF
Sbjct: 99 FKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKD 158
Query: 563 LYTYVN 580
+ + N
Sbjct: 159 IVSGSN 164
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 35.1 bits (77), Expect = 0.011
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 9/98 (9%)
Frame = +2
Query: 380 QVQQWFRAVDKD-QSGFITSTELRSALVNAQGQTFSETAC--NLMIGMFDKDRNGQINLD 550
++QQW++ KD SG + +E + + Q F + + + +FD D+NG I+
Sbjct: 26 ELQQWYKGFFKDCPSGHLNKSEFQK--IYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFK 83
Query: 551 EFDKLYTYV------NQWLAVFKTYDTDQSGFIDEGEL 646
EF + ++ + F+ YD D +G I E+
Sbjct: 84 EFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEM 121
Score = 28.3 bits (60), Expect = 1.3
Identities = 26/91 (28%), Positives = 38/91 (41%), Gaps = 9/91 (9%)
Frame = +2
Query: 509 GMFDKDRNGQINLDEFDKLYTYVNQW-------LAVFKTYDTDQSGFIDEGELSKAL--T 661
G F +G +N EF K+Y + VF +D D++G+ID E AL T
Sbjct: 33 GFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVT 92
Query: 662 QMGFRFSPEFINILTKRSNTNDGKISVDXFI 754
G + + I N+G IS D +
Sbjct: 93 SRG-ELNDKLIWAFQLYDLDNNGLISYDEML 122
Score = 27.1 bits (57), Expect = 3.0
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +2
Query: 485 ETACNLMIGMFDKDRNGQINLDEF 556
E N + M DK+++GQ+ L+EF
Sbjct: 146 EKRVNKIFNMMDKNKDGQLTLEEF 169
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 33.1 bits (72), Expect = 0.045
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +2
Query: 596 FKTYDTDQSGFIDEGELSKALTQMGFRF-SPEFINILTKRSNTNDGKISVDXFI 754
FK +D+D+ ID EL A+ +GF E + IL T G + ++ F+
Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFV 96
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 32.3 bits (70), Expect = 0.078
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +2
Query: 596 FKTYDTDQSGFIDEGELSKALTQMGFRFSPEFINILTKRSNTNDGKISVDXF 751
F +D G+ID +L ++ Q+G + E + ++ + TN GK+S + F
Sbjct: 18 FDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTN-GKVSREEF 68
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 29.9 bits (64), Expect = 0.42
Identities = 15/54 (27%), Positives = 26/54 (48%)
Frame = +2
Query: 395 FRAVDKDQSGFITSTELRSALVNAQGQTFSETACNLMIGMFDKDRNGQINLDEF 556
FR DKD SG+I + + + + G+ S+ LM+ D +G + +F
Sbjct: 84 FRVFDKDNSGYIETAKF-ADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDF 136
>SPCC4B3.13 |||MatE family transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 539
Score = 25.8 bits (54), Expect = 6.8
Identities = 20/66 (30%), Positives = 29/66 (43%)
Frame = +2
Query: 287 GQAAYGGMPPGQLEIGHGPYPSIGVGGTITPQVQQWFRAVDKDQSGFITSTELRSALVNA 466
G+ A+GG L+ GP + V G I + W + SG + +TEL S V +
Sbjct: 304 GRQAWGGFSREALK-NWGPLCRLAVPGVIMICSEYWAFELVTFASGVLGTTELASMSVLS 362
Query: 467 QGQTFS 484
T S
Sbjct: 363 TTSTLS 368
>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1115
Score = 25.8 bits (54), Expect = 6.8
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = -3
Query: 526 VFVEHTNHQIASSFGKSLT 470
V+ H N QI SSFG SLT
Sbjct: 33 VWTGHKNGQIKSSFGPSLT 51
>SPAC18G6.04c |shm2||serine hydroxymethyltransferase Shm2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 472
Score = 25.4 bits (53), Expect = 9.0
Identities = 9/17 (52%), Positives = 11/17 (64%)
Frame = +2
Query: 257 LQMAYNSGYPGQAAYGG 307
+Q Y+ GYPG YGG
Sbjct: 61 MQNKYSEGYPGARYYGG 77
>SPBC30D10.10c |tor1||phosphatidylinositol kinase
Tor1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2335
Score = 25.4 bits (53), Expect = 9.0
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = -2
Query: 527 GLCRTYQSSDCKQFRKKFDLER 462
GLC T ++D + F+++ ++ER
Sbjct: 2002 GLCNTLLTTDSETFKRRLNIER 2023
>SPBC17A3.01c |tim50|SPBC8D2.21c|TIM23 translocase complex subunit
Tim50 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.4 bits (53), Expect = 9.0
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -2
Query: 353 WKGTVHVQFLIGLVAFHRKLPVLDIRNCRPFVVS 252
W G + L+GL+ + ++DI++ RP + S
Sbjct: 297 WTGNPKDKELVGLIPLLEFIAIMDIKDVRPVLKS 330
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,174,477
Number of Sequences: 5004
Number of extensions: 66978
Number of successful extensions: 164
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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