BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P16_pT_N05
(530 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 40 3e-04
SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 32 0.061
SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces po... 28 0.76
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 26 3.0
SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr 3|||Ma... 26 4.0
SPBC1271.02 |stt3||oligosaccharyltransferase subunit Stt3|Schizo... 25 7.0
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.0
SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 25 9.3
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 39.5 bits (88), Expect = 3e-04
Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 3/70 (4%)
Frame = -2
Query: 457 NYIIPKGTFILLSLY-ELHHGPHWKDPETFRPERFLTKEGNILQD--EWLIPFGIGKRRC 287
+Y +PK ++ +LY LH + +PETF P+R+ G Q W++ FG G C
Sbjct: 402 DYTVPKDAMVIPTLYGALHDSKVYPEPETFNPDRW-APNGLAEQSPKNWMV-FGNGPHVC 459
Query: 286 IGEGLARSEL 257
+G+ A + L
Sbjct: 460 LGQRYAVNHL 469
>SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 495
Score = 31.9 bits (69), Expect = 0.061
Identities = 15/31 (48%), Positives = 20/31 (64%)
Frame = -2
Query: 316 IPFGIGKRRCIGEGLARSELFMFLTHILQKF 224
+PFG G+ RCIGE A +M L+ I+ KF
Sbjct: 433 LPFGAGRHRCIGEQFA----YMHLSTIISKF 459
>SPBC1604.18c |||vacuolar sorting protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 449
Score = 28.3 bits (60), Expect = 0.76
Identities = 20/60 (33%), Positives = 30/60 (50%)
Frame = +3
Query: 201 SFLGILRWNF*RI*VRNMKSSERANPSPIQRRFPIPKGMSHSSCKMLPSLVRNRSGRNVS 380
S ILRW+F ++ +N SS +N SP ++ I K + +C LV N + R S
Sbjct: 124 SIFSILRWSFQKLGFQNQASSILSNRSP-SGQYVIRKNIEKLAC-----LVHNEAMRRCS 177
>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 595
Score = 26.2 bits (55), Expect = 3.0
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +3
Query: 249 NMKSSERANPSPIQRRFPIPKGMSHSSCKMLPSLVRN 359
N + E+ N P+Q P+P MS +S + S + N
Sbjct: 41 NRLAVEKPNIQPLQGHSPVPSFMSTASTNISSSKINN 77
>SPCC338.17c |rad21||kleisin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 628
Score = 25.8 bits (54), Expect = 4.0
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -3
Query: 174 MDFHCPLSNSGLFSNREKH 118
M FH P+SNSG + R H
Sbjct: 200 MQFHLPISNSGAATPRSVH 218
>SPBC1271.02 |stt3||oligosaccharyltransferase subunit
Stt3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 752
Score = 25.0 bits (52), Expect = 7.0
Identities = 11/39 (28%), Positives = 19/39 (48%)
Frame = -2
Query: 517 ISTVASMGIPHMALNDAKLGNYIIPKGTFILLSLYELHH 401
I T+ASM +P + ++ G F LL L+ ++
Sbjct: 252 IGTLASMQVPFVGFQPVSTSEHMSALGVFGLLQLFAFYN 290
>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 386
Score = 25.0 bits (52), Expect = 7.0
Identities = 13/29 (44%), Positives = 16/29 (55%)
Frame = -2
Query: 214 IPKNEPLPSTEPIDGLSLSAKQFRIIFEP 128
+P N +P TEP+D SLS Q R P
Sbjct: 190 LPSNS-IPKTEPLDTPSLSNSQERFSKSP 217
>SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 567
Score = 24.6 bits (51), Expect = 9.3
Identities = 13/36 (36%), Positives = 17/36 (47%)
Frame = +3
Query: 339 LPSLVRNRSGRNVSGSFQCGP*CSSYSDNKIKVPFG 446
L S R N S S P S +SD ++ VP+G
Sbjct: 207 LVSAGNGRETVNASASGAVNPTSSQWSDQRLGVPYG 242
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,255,107
Number of Sequences: 5004
Number of extensions: 49263
Number of successful extensions: 150
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 218398248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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