Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P16_pT_J16
(728 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 265 5e-72
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 265 5e-72
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 181 8e-47
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 100 4e-22
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 45 1e-05
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 27 2.1
SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 27 2.7
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 27 2.7
SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.3
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 8.4
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 8.4
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 25 8.4
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 265 bits (649), Expect = 5e-72
Identities = 124/187 (66%), Positives = 139/187 (74%)
Frame = -3
Query: 726 VDCSKGVQYLNEIKDSVVXGFQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQII 547
VD +K V YLNEIKDSVV F WA KEG M EENLR RFN DV LH DAIHRGGGQII
Sbjct: 647 VDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQII 706
Query: 546 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMF 367
PT RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++
Sbjct: 707 PTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLY 766
Query: 366 IVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRK 187
+KAYLPVNESFGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRK
Sbjct: 767 NIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRK 826
Query: 186 GLKEGLP 166
GLKE +P
Sbjct: 827 GLKENVP 833
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 265 bits (649), Expect = 5e-72
Identities = 124/187 (66%), Positives = 139/187 (74%)
Frame = -3
Query: 726 VDCSKGVQYLNEIKDSVVXGFQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQII 547
VD +K V YLNEIKDSVV F WA KEG M EENLR RFN DV LH DAIHRGGGQII
Sbjct: 647 VDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQII 706
Query: 546 PTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMF 367
PT RR +YA L A P + EPV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++
Sbjct: 707 PTARRVVYASTLLASPIIQEPVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLY 766
Query: 366 IVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRK 187
+KAYLPVNESFGFT +LR T GQAFPQ VFDHW + GDP +P SKP +V E RKRK
Sbjct: 767 NIKAYLPVNESFGFTGELRQATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRK 826
Query: 186 GLKEGLP 166
GLKE +P
Sbjct: 827 GLKENVP 833
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 181 bits (441), Expect = 8e-47
Identities = 84/168 (50%), Positives = 111/168 (66%)
Frame = -3
Query: 723 DCSKGVQYLNEIKDSVVXGFQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQIIP 544
D K V LN +K+ + GFQW +EG + +E +R V F DV L + I+RGGGQIIP
Sbjct: 766 DVDKNV--LNSVKEYIKQGFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIP 823
Query: 543 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 364
T RR Y+ LTA PRLMEPVY+ E+ P ++ IY +L RRRGHV ++ G+P+++
Sbjct: 824 TARRVCYSSFLTASPRLMEPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYL 883
Query: 363 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDPCEPQSKP 220
V+A +PV +S GF DLR +T GQA Q VFDHWQV+PGDP + KP
Sbjct: 884 VRALIPVIDSCGFETDLRVHTQGQAMCQMVFDHWQVVPGDPLDKSIKP 931
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 99.5 bits (237), Expect = 4e-22
Identities = 54/161 (33%), Positives = 88/161 (54%), Gaps = 9/161 (5%)
Frame = -3
Query: 696 NEIKDSVVXGFQWAXKEGVMAEENLRG--VRFNXYDVT-------LHTDAIHRGGGQIIP 544
+++ + VV FQ +G + E ++G V + +D++ L T + GQ+I
Sbjct: 786 SDLSEYVVTAFQLITHQGPLCAEPVQGICVSIDQFDISDDSEDSKLLTINNPQIPGQVIS 845
Query: 543 TTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFI 364
+ + L PRLM +Y C++Q +G +YGV+++RRG V +E GTP FI
Sbjct: 846 VVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEGTPFFI 905
Query: 363 VKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 241
VKA +PV ESFGF ++ T G A+PQ +F +++L +P
Sbjct: 906 VKALIPVVESFGFAVEILKRTSGAAYPQLIFHGFEMLDENP 946
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 45.2 bits (102), Expect = 1e-05
Identities = 30/125 (24%), Positives = 55/125 (44%)
Frame = -3
Query: 666 FQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 487
F A K+G + ++ RF D H ++ T L A P ++E
Sbjct: 619 FYEALKKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRL--ATISAFRTAFLQANPMVLE 676
Query: 486 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 307
P+ I P GG+ G L++R+ + + F ++A +P+N F +++D+R+
Sbjct: 677 PIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRA 734
Query: 306 NTGGQ 292
T G+
Sbjct: 735 LTKGK 739
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 27.5 bits (58), Expect = 2.1
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +2
Query: 539 VVGMIWPPPLWMASVWSVTSXMLNLTPRKFSSAITPSLXA 658
V G+ PPPL + S+ + + + + FS A+ PSL A
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344
>SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 536
Score = 27.1 bits (57), Expect = 2.7
Identities = 30/139 (21%), Positives = 54/139 (38%), Gaps = 4/139 (2%)
Frame = -1
Query: 656 PXRKELWLKRICVVLDSTXMM*HSILMPSIEV-VAKSFQQLEDACTHVC*LLS--PVLWS 486
P K+ W CV L+ H+ + P+++V + + D+ + ++ PV
Sbjct: 50 PQLKQTWQFNFCVDLNFLLENMHASVFPTVDVRITHGYDSKSDSLARLTAQMNHCPVNVK 109
Query: 485 LYIFVKFSVLK*LWXXXXXXXXXXXXTFSKSPRWQVHLCSL*RPTYLSMSRSVL-LPICV 309
LY SV +W S + +H +L P ++ MS+++ P+
Sbjct: 110 LY-----SVYVPMWGTHHSKIMVNFFK-DDSCQIVIHTANLVEPDWIGMSQAIFKTPLLY 163
Query: 308 PTPADRPSRSAYSTIGRSS 252
P D S S+ G S
Sbjct: 164 PKANDSLSTSSVPEYGNPS 182
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 27.1 bits (57), Expect = 2.7
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +2
Query: 563 PLWMASVWSVTSXMLNLTPRKFSSAITPSLXAHWNPXTTESLISLRY 703
P W+A V ++ S + P FSS + +L A+ N + + + Y
Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPNSVSIRDITTHYY 89
>SPAC23H4.16c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 328
Score = 25.8 bits (54), Expect = 6.3
Identities = 23/83 (27%), Positives = 39/83 (46%), Gaps = 7/83 (8%)
Frame = +1
Query: 463 LNFTKIYRLHKTGLSS*QTCVQASSSCWND-----LAT--TSMDGISMECYIIXVESNTT 621
L+++K++ LH +Q S S +ND LA ++ + + Y++ E +
Sbjct: 192 LSYSKVFFLH---------LLQHSDSSFNDKFLLLLANIPVTVSNVEVLLYLLQQEESLA 242
Query: 622 QILFSHNSFLXGPLESXHNRVLN 690
Q + SFL L S HN+V N
Sbjct: 243 QFDLNGKSFLYHMLVSLHNQVTN 265
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 25.4 bits (53), Expect = 8.4
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -3
Query: 267 HWQVLPGDPCEPQSKPYNVVQETRKRK 187
H+Q L GDP + PY VQ RK
Sbjct: 78 HFQNLSGDPVSQMATPYPPVQIELMRK 104
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 8.4
Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = -3
Query: 549 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 373
IP T RC+ + + + P+L P L + CP + + V+ H F + P
Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444
Query: 372 MFIVKAY 352
+K+Y
Sbjct: 445 ALALKSY 451
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 541 CWNDLATTSMDGISMECYIIXVESNTTQI 627
CW++L+TTS + + II + TT I
Sbjct: 211 CWDELSTTSPESSKVSEPIIQDNTQTTHI 239
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,098,705
Number of Sequences: 5004
Number of extensions: 65675
Number of successful extensions: 151
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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