Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P16_F_O14
(769 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q29R09 Cluster: LD28546p; n=7; Coelomata|Rep: LD28546p ... 192 6e-48
UniRef50_Q86M58 Cluster: Ubiquitin conjugating enzyme UBC-25; n=... 124 3e-27
UniRef50_Q7Z7E8 Cluster: Ubiquitin-conjugating enzyme E2 Q1; n=6... 120 3e-26
UniRef50_UPI0000F2BDCC Cluster: PREDICTED: similar to ubiquitin-... 107 2e-22
UniRef50_Q4S863 Cluster: Chromosome 5 SCAF14709, whole genome sh... 80 7e-14
UniRef50_Q4S862 Cluster: Chromosome 5 SCAF14709, whole genome sh... 73 6e-12
UniRef50_UPI0000E1FDC5 Cluster: PREDICTED: similar to ubiquitin-... 50 5e-05
UniRef50_A3HAJ0 Cluster: Putative uncharacterized protein; n=1; ... 36 1.1
UniRef50_Q54KZ9 Cluster: Putative uncharacterized protein; n=1; ... 34 4.5
UniRef50_Q4SH76 Cluster: Chromosome 8 SCAF14587, whole genome sh... 33 5.9
UniRef50_A6LYY8 Cluster: 4Fe-4S ferredoxin, iron-sulfur binding ... 33 7.8
UniRef50_A5M4D7 Cluster: Phage protein; n=1; Streptococcus pneum... 33 7.8
>UniRef50_Q29R09 Cluster: LD28546p; n=7; Coelomata|Rep: LD28546p -
Drosophila melanogaster (Fruit fly)
Length = 397
Score = 192 bits (469), Expect = 6e-48
Identities = 85/108 (78%), Positives = 98/108 (90%)
Frame = +2
Query: 113 MACLNPLKLAIKTLXQVFPKNHERFQIMSASVDELTCRFVGKNGKKYEIHANITETYPTT 292
MACLN LK IKTL ++FPKNHERFQI+++SVDEL CRF+ KNGK+Y+IHANITETYP++
Sbjct: 1 MACLNTLKQEIKTLEKIFPKNHERFQILNSSVDELLCRFIDKNGKRYDIHANITETYPSS 60
Query: 293 PPVWFAESEDPIVTNAVQILTNTQGRDNHVINQVGILLRELCKLHGVP 436
PPVWFAESE+ VTNAVQIL+NT GRDNHVINQVGILLRELC+LH VP
Sbjct: 61 PPVWFAESEETSVTNAVQILSNTNGRDNHVINQVGILLRELCRLHNVP 108
Score = 97.5 bits (232), Expect = 3e-19
Identities = 47/60 (78%), Positives = 51/60 (85%)
Frame = +2
Query: 590 LPLEMVEDAGRSNKDDMETEHLATLERLXQNQRQDYLSGSVSGSLQATDRLMKELRDIYR 769
LPLEM + S KDDME EHLATLE+L Q+QRQDYL GSVSGS+QATDRLMKELRDIYR
Sbjct: 177 LPLEMDDVRSTSKKDDMEVEHLATLEKLRQSQRQDYLKGSVSGSVQATDRLMKELRDIYR 236
>UniRef50_Q86M58 Cluster: Ubiquitin conjugating enzyme UBC-25; n=3;
Caenorhabditis|Rep: Ubiquitin conjugating enzyme UBC-25
- Caenorhabditis elegans
Length = 387
Score = 124 bits (298), Expect = 3e-27
Identities = 79/229 (34%), Positives = 116/229 (50%), Gaps = 10/229 (4%)
Frame = +2
Query: 113 MACLNPLKLAIKTLXQVFPKNHERFQIMSASVDELTCRFVGKNGKKYEIHANITETYPTT 292
MACL LK I+ L ++FPKNH RFQI+SASVDEL+ +F+ K + ANI E YP
Sbjct: 1 MACLRKLKEDIQVLEKLFPKNHNRFQILSASVDELSMKFINAENKGIIVTANIQENYPRQ 60
Query: 293 PPVWFAESED-PIVTNAVQILTNTQGRDNHVINQVGILLRELCKLHGVPEXXXXXXXXXX 469
PP+WF+ES+D P++ ++Q LT T+ N +++QV L+ +LC + +
Sbjct: 61 PPIWFSESDDVPVIGMSLQRLTETEESTN-ILHQVHRLVSDLCSFYNLQMPCELPQIAPP 119
Query: 470 VHPAPPQHR---VPSVTSNXXXXXXXXXXXXXXXXXXXXXXXXL--PLEMVE----DAGR 622
V + R + TS +E+VE D
Sbjct: 120 VRDDIDEGRGSDISDTTSEPIDDDMAGDGEVDDDDEEEEDDEDADGDIEIVEMAEEDPTS 179
Query: 623 SNKDDMETEHLATLERLXQNQRQDYLSGSVSGSLQATDRLMKELRDIYR 769
+ + E L L++ + RQ +L G V GS+ A+DRLMKE+RDI+R
Sbjct: 180 QHDVGVSKEGLDMLDKASKINRQQHLDGKVQGSITASDRLMKEIRDIHR 228
>UniRef50_Q7Z7E8 Cluster: Ubiquitin-conjugating enzyme E2 Q1; n=66;
Eumetazoa|Rep: Ubiquitin-conjugating enzyme E2 Q1 - Homo
sapiens (Human)
Length = 422
Score = 120 bits (290), Expect = 3e-26
Identities = 79/236 (33%), Positives = 119/236 (50%), Gaps = 23/236 (9%)
Frame = +2
Query: 131 LKLAIKTLXQVFPKNHERFQIMSASVDELTCRFV-------------GKN--------GK 247
L+ +K L +F + HERF+I SA +DEL+C F+ G + G
Sbjct: 41 LRRELKLLESIFHRGHERFRIASACLDELSCEFLLAGAGGAGAGAAPGPHLPPRGSVPGD 100
Query: 248 KYEIHANITETYPTTPPVWFAESEDPIVTNAVQILTNTQGRDNHVINQVGILLRELCKLH 427
IH NITE+YP PP+W ES+DP + ++ L + + + ++ + ++ +LCKL+
Sbjct: 101 PVRIHCNITESYPAVPPIWSVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLY 160
Query: 428 GVPEXXXXXXXXXXVHPAPPQHRVPSVTSNXXXXXXXXXXXXXXXXXXXXXXXXLPLEMV 607
+P+ HP P ++
Sbjct: 161 NLPQ-----------HPDVEMLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEE 209
Query: 608 EDA-GRSNKDD-METEHLATLERLXQNQRQDYLSGSVSGSLQATDRLMKELRDIYR 769
E A G+ ++DD + E+LA LE++ +NQRQDYL+G+VSGS+QATDRLMKELRDIYR
Sbjct: 210 EPAEGKKSEDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYR 265
>UniRef50_UPI0000F2BDCC Cluster: PREDICTED: similar to
ubiquitin-conjugating enzyme E2Q; n=1; Monodelphis
domestica|Rep: PREDICTED: similar to
ubiquitin-conjugating enzyme E2Q - Monodelphis domestica
Length = 547
Score = 107 bits (258), Expect = 2e-22
Identities = 73/219 (33%), Positives = 110/219 (50%), Gaps = 23/219 (10%)
Frame = +2
Query: 182 RFQIMSASVDELTCRFV-------------GKN--------GKKYEIHANITETYPTTPP 298
RF+I SA +DEL+C F+ G + G+ IH NITE+YP PP
Sbjct: 183 RFRIASACLDELSCEFLLAGAGAGAAGAAPGPHLPPRGPAPGEPVRIHCNITESYPAVPP 242
Query: 299 VWFAESEDPIVTNAVQILTNTQGRDNHVINQVGILLRELCKLHGVPEXXXXXXXXXXVHP 478
+W ES+DP + ++ L + + + ++ + ++ +LCKL+ +P+ HP
Sbjct: 243 IWSVESDDPNLAAVLERLVDIKKGNTLLLQHLKRIISDLCKLYNLPQ-----------HP 291
Query: 479 APPQHRVPSVTSNXXXXXXXXXXXXXXXXXXXXXXXXLPLEMVEDA-GRSNKDD-METEH 652
P ++ E A G+ ++DD + E+
Sbjct: 292 DVEMLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEEPAEGKKSEDDGIGKEN 351
Query: 653 LATLERLXQNQRQDYLSGSVSGSLQATDRLMKELRDIYR 769
LA LE++ +NQRQDYL+G+VSGS+QATDRLMKELRDIYR
Sbjct: 352 LAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYR 390
>UniRef50_Q4S863 Cluster: Chromosome 5 SCAF14709, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 5 SCAF14709, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 145
Score = 79.8 bits (188), Expect = 7e-14
Identities = 34/78 (43%), Positives = 50/78 (64%)
Frame = +2
Query: 131 LKLAIKTLXQVFPKNHERFQIMSASVDELTCRFVGKNGKKYEIHANITETYPTTPPVWFA 310
LK +K L +F NHERF+I+ DEL+C+F K IH NITE+YP+TPP+WF
Sbjct: 19 LKAELKFLESIFDPNHERFRIIDWKPDELSCQFNVTGDKLLIIHCNITESYPSTPPIWFV 78
Query: 311 ESEDPIVTNAVQILTNTQ 364
+S+DP + ++ L + +
Sbjct: 79 DSDDPSLAQVLERLDDVR 96
>UniRef50_Q4S862 Cluster: Chromosome 5 SCAF14709, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 5 SCAF14709, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 297
Score = 73.3 bits (172), Expect = 6e-12
Identities = 33/53 (62%), Positives = 45/53 (84%)
Frame = +2
Query: 611 DAGRSNKDDMETEHLATLERLXQNQRQDYLSGSVSGSLQATDRLMKELRDIYR 769
D +S D +E E+LA LE++ +NQRQD+L+G+VSGS+QA+DRLMKELR+IYR
Sbjct: 23 DGKKSEDDGIEKENLAILEKIRKNQRQDHLNGAVSGSVQASDRLMKELREIYR 75
>UniRef50_UPI0000E1FDC5 Cluster: PREDICTED: similar to
ubiquitin-conjugating enzyme UBCi; n=1; Pan
troglodytes|Rep: PREDICTED: similar to
ubiquitin-conjugating enzyme UBCi - Pan troglodytes
Length = 115
Score = 50.4 bits (115), Expect = 5e-05
Identities = 29/82 (35%), Positives = 46/82 (56%), Gaps = 6/82 (7%)
Frame = +2
Query: 131 LKLAIKTLXQVFPKNHERFQIMSASVDELTCRF-VGKNGKKY-----EIHANITETYPTT 292
L L ++ +F KN ++F+I+S +DEL C+F V G + +H NITE+YP +
Sbjct: 16 LLLKYQSPEHIFSKNRKQFRIISWKLDELHCQFPVLLPGSLHLLPLLTLHCNITESYPPS 75
Query: 293 PPVWFAESEDPIVTNAVQILTN 358
+WF S+DP T ++ N
Sbjct: 76 SLMWFVGSDDPNRTLVLECTKN 97
>UniRef50_A3HAJ0 Cluster: Putative uncharacterized protein; n=1;
Caldivirga maquilingensis IC-167|Rep: Putative
uncharacterized protein - Caldivirga maquilingensis
IC-167
Length = 213
Score = 35.9 bits (79), Expect = 1.1
Identities = 24/85 (28%), Positives = 43/85 (50%), Gaps = 5/85 (5%)
Frame = +2
Query: 152 LXQVFPKNHERFQIMSASVDELTCRFVG--KNGKKYEIHANITETYPTTPPVWFAESEDP 325
+ QV N + ++S ++D L+ G ++G +Y IH ++ + T P + +
Sbjct: 2 IRQVKVVNGDLILVLSNAMDSLSSTVDGGLRHGIRYIIHHHVPSNFNTDPMLEVRRVHEA 61
Query: 326 IVTN---AVQILTNTQGRDNHVINQ 391
++ N AV LT T+ NHVIN+
Sbjct: 62 LLINSNEAVTFLTATELPKNHVINE 86
>UniRef50_Q54KZ9 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 417
Score = 33.9 bits (74), Expect = 4.5
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = -2
Query: 300 TGGVVGYVSVMFACISYFFPFLPTNLQVSSSTLADII*KRSWFFG 166
+GGVVGY+ C+ + F PT ++++ +T+ ++ S FG
Sbjct: 242 SGGVVGYIVGFALCLFIMYSFTPTMMEIAGATVMNLSLLTSDMFG 286
>UniRef50_Q4SH76 Cluster: Chromosome 8 SCAF14587, whole genome
shotgun sequence; n=2; Tetraodontidae|Rep: Chromosome 8
SCAF14587, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 730
Score = 33.5 bits (73), Expect = 5.9
Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 6/64 (9%)
Frame = +2
Query: 98 CSHTLMACLNPLKLAIKTLXQV---FPK---NHERFQIMSASVDELTCRFVGKNGKKYEI 259
C H CL+P L QV FPK N E FQ + +L C++ GK Y++
Sbjct: 63 CQHCSFKCLSPGHLKASAFQQVFTSFPKTQLNLESFQQLKKIYGDLECQYCGKL-FWYKV 121
Query: 260 HANI 271
H N+
Sbjct: 122 HYNV 125
>UniRef50_A6LYY8 Cluster: 4Fe-4S ferredoxin, iron-sulfur binding
domain protein; n=5; Clostridium|Rep: 4Fe-4S ferredoxin,
iron-sulfur binding domain protein - Clostridium
beijerinckii NCIMB 8052
Length = 281
Score = 33.1 bits (72), Expect = 7.8
Identities = 23/89 (25%), Positives = 43/89 (48%), Gaps = 7/89 (7%)
Frame = +2
Query: 191 IMSASVDELTCRFVGKNGKKYEIHANITETYPTTPPVWFAESEDPIVTNAVQILTNTQGR 370
+M + +L F+ K+YE NI+ + P V FA++ DP + N +++T T
Sbjct: 15 VMKEKITKLIKNFI----KEYERQDNISTRWGD-PLVGFADANDPYILNFKELITPTHKL 69
Query: 371 DNHVINQVGILL-------RELCKLHGVP 436
V++ I++ +EL + +G P
Sbjct: 70 PTDVLSDASIVIAYFVPFTKELARTNGTP 98
>UniRef50_A5M4D7 Cluster: Phage protein; n=1; Streptococcus
pneumoniae SP11-BS70|Rep: Phage protein - Streptococcus
pneumoniae SP11-BS70
Length = 821
Score = 33.1 bits (72), Expect = 7.8
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = -2
Query: 366 P*VLVSI*TAFVTIGSSLSANQTGGVVGYVSVMFACISYFFPFLPTNLQVSSSTLADII* 187
P VL +I TA + + +SAN G + ++ + A + YFF T Q+ SS +A I
Sbjct: 339 PAVLTTIKTAVTGLFTVMSANPIGIAIAAIAGLTAGLVYFFTQTETGRQIWSSFVAWI-- 396
Query: 186 KRSW 175
K++W
Sbjct: 397 KQAW 400
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 734,784,002
Number of Sequences: 1657284
Number of extensions: 13880483
Number of successful extensions: 37130
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 35816
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37112
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 64204279620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -