Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P16_F_E16
         (733 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit...    64   3e-11
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    55   9e-09
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc...    29   0.68 
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo...    29   0.68 
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M...    27   2.1  
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch...    27   2.8  
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar...    27   3.6  
SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   4.8  
SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit...    25   8.4  
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb...    25   8.4  
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy...    25   8.4  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    25   8.4  

>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
           Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 354

 Score = 63.7 bits (148), Expect = 3e-11
 Identities = 26/79 (32%), Positives = 54/79 (68%)
 Frame = +3

Query: 417 EAKEQKRINQEIERQLRKDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKR 596
           E+ E K++N +IE+Q+    +  ++  K+LLLG  +SGKST  KQ++I++ +G+S E+  
Sbjct: 9   ESGESKKLNSKIEKQIENASKKDKKIYKVLLLGASDSGKSTISKQIKILNKNGFSQEEIM 68

Query: 597 GFIKLVYQNIFMAMQSMIR 653
            FI ++ +N+  + +++++
Sbjct: 69  TFIPVIRRNLLESAKTLVK 87


>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 55.2 bits (127), Expect = 9e-09
 Identities = 21/57 (36%), Positives = 42/57 (73%)
 Frame = +3

Query: 492 ELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFIKLVYQNIFMAMQSMIRAMD 662
           ++K+LLLG G+SGK+T +KQMR+++  G+S   ++ +  ++++NI  ++  ++ AMD
Sbjct: 74  DIKVLLLGAGDSGKTTIMKQMRLLYSPGFSQVVRKQYRVMIFENIISSLCLLLEAMD 130


>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1085

 Score = 29.1 bits (62), Expect = 0.68
 Identities = 13/46 (28%), Positives = 26/46 (56%)
 Frame = +3

Query: 423 KEQKRINQEIERQLRKDKRDARRELKLLLLGTGESGKSTFIKQMRI 560
           +EQ++  QE+ERQ R++K+  +   K L     E+ +    ++ R+
Sbjct: 648 REQEKKQQELERQKREEKQKQKEREKKLKKQQQEADREKMAREQRL 693


>SPAC17C9.03 |tif471||translation initiation factor eIF4G
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1403

 Score = 29.1 bits (62), Expect = 0.68
 Identities = 19/72 (26%), Positives = 28/72 (38%)
 Frame = +1

Query: 415 KKQRNKKGSIKK*RGNSERTNEMPEENSNCSYLELESRANLLSSSR*ESSTARVIATKTS 594
           K   NK+GS++K       TN  P    N S     S A   SS   +  T+  I    +
Sbjct: 82  KSPVNKEGSVEKKSSEKSSTNNKPWRGDNTSKPSANSSAERTSSQHQKPETSSQIGKDNA 141

Query: 595 VASSNSFTRTSS 630
               N   +++S
Sbjct: 142 APVENVNEKSTS 153


>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 500

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 26/101 (25%), Positives = 50/101 (49%), Gaps = 4/101 (3%)
 Frame = +1

Query: 322 SVERPAERTGL--RARGQIAALRSEKSWSAACQKKQRNKKGSIK--K*RGNSERTNEMPE 489
           ++E+P++   +   A  +IA   S+   S    KK+  +  S +  K     ++ ++  E
Sbjct: 22  AIEKPSKSKKITKEAAKEIAKQSSKTDVSPKKSKKEAKRASSPEPSKKSVKKQKKSKKKE 81

Query: 490 ENSNCSYLELESRANLLSSSR*ESSTARVIATKTSVASSNS 612
           E+S+ S  E  S  +  SSS  ESS++   ++ +  +SS S
Sbjct: 82  ESSSESESESSSSESESSSSESESSSSESESSSSESSSSES 122


>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 216

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 10/18 (55%), Positives = 16/18 (88%)
 Frame = +3

Query: 498 KLLLLGTGESGKSTFIKQ 551
           KL+L+G G +GK+TF+K+
Sbjct: 11  KLVLVGDGGTGKTTFVKR 28


>SPAC22F3.05c |alp41||ADP-ribosylation factor
           Alp41|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 186

 Score = 26.6 bits (56), Expect = 3.6
 Identities = 13/29 (44%), Positives = 20/29 (68%)
 Frame = +3

Query: 462 LRKDKRDARRELKLLLLGTGESGKSTFIK 548
           LR+ K    RE+++LLLG   +GK+T +K
Sbjct: 7   LRQQKLK-EREVRVLLLGLDNAGKTTILK 34


>SPAC4H3.14c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 345

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 12/28 (42%), Positives = 21/28 (75%)
 Frame = +3

Query: 420 AKEQKRINQEIERQLRKDKRDARRELKL 503
           AKE+++I +E ERQ+  D  + +++LKL
Sbjct: 134 AKEEQKIQEEFERQI-TDLLEEQQQLKL 160


>SPBC1718.03 |ker1||DNA-directed RNA polymerase I complex subunit
           subunit Ker1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 11/40 (27%), Positives = 23/40 (57%)
 Frame = +3

Query: 417 EAKEQKRINQEIERQLRKDKRDARRELKLLLLGTGESGKS 536
           + + +KR+ +E ++  R++K +AR+       G GE  +S
Sbjct: 108 DKETKKRLKKEKKKAARREKEEARKAKADTTQGVGEKEQS 147


>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 695

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 7/62 (11%)
 Frame = +3

Query: 408 MSEEAKEQKRINQE-------IERQLRKDKRDARRELKLLLLGTGESGKSTFIKQMRIIH 566
           +S++ K QK  N          + QL +  +  +   KLLLLG  +SGK T + ++    
Sbjct: 245 VSQQLKPQKNTNDSNNDHTLLSQDQLIELSKLVKPRTKLLLLGPPKSGKKTLLSRLFFQI 304

Query: 567 GS 572
           GS
Sbjct: 305 GS 306


>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 180

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 7/25 (28%), Positives = 20/25 (80%)
 Frame = +3

Query: 486 RRELKLLLLGTGESGKSTFIKQMRI 560
           +RE+++L++G   +GK+T + ++++
Sbjct: 15  KREMRILMVGLDAAGKTTILYKLKL 39


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1957

 Score = 25.4 bits (53), Expect = 8.4
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = +3

Query: 408 MSEEAKEQKRINQEIERQLRKDKRDARRELK 500
           + E+ KEQK++++  +  L+  +RD   E K
Sbjct: 495 LREQIKEQKKVSESTQSSLQSLQRDILNEKK 525


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,872,745
Number of Sequences: 5004
Number of extensions: 59250
Number of successful extensions: 203
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)