Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P16_F_E14
         (551 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...   141   7e-35
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc...    26   3.2  
SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual             25   5.6  
SPBC23G7.08c |rga7||GTPase activating protein Rga7|Schizosacchar...    25   5.6  
SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p...    25   7.4  
SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces po...    25   7.4  
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc...    25   9.8  
SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ...    25   9.8  

>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score =  141 bits (341), Expect = 7e-35
 Identities = 80/182 (43%), Positives = 101/182 (55%), Gaps = 1/182 (0%)
 Frame = +2

Query: 8   VFXQVGKAP-AANLPHVLRWYNQIASYTSAERKTWSQGTSPLXXXXXXXXXXXXXXXXXX 184
           VF  VG AP  A  P+  RWY QIA+Y  A        T P                   
Sbjct: 37  VFKAVGVAPDTAKYPNGARWYKQIATYDLA--------TLPGTAKEVSAYGPEGAAAAEE 88

Query: 185 XXVDLFGSGXXXXXXXXXXXXXXXLKAYADKKSKKPALIAKSSILLDVKPWDDETDMKEM 364
             +DLFGS                 + Y  KK+ KP  + KS + LDVKPWDDET M E+
Sbjct: 89  DEIDLFGSDEEEDPEAERIKAERVAE-YNKKKAAKPKAVHKSLVTLDVKPWDDETPMDEL 147

Query: 365 ENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSVDLLTEKIQEFEXFVQSVD 544
           E  VR+I+M+GL+WG SKLVPVG+G+NK QI  V+EDDKVS++ L E+++ FE +VQS D
Sbjct: 148 EKAVRSIQMDGLVWGLSKLVPVGFGVNKFQINLVVEDDKVSLEALQEELEGFEDYVQSTD 207

Query: 545 IA 550
           IA
Sbjct: 208 IA 209


>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1016

 Score = 26.2 bits (55), Expect = 3.2
 Identities = 14/37 (37%), Positives = 18/37 (48%), Gaps = 1/37 (2%)
 Frame = -2

Query: 418 FGGSPEKAFHFNSAYLVFHFLHIGFIIP-WLDIKENR 311
           FG S     HF+  Y VF    IG I P W++   N+
Sbjct: 485 FGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNK 521


>SPAC3F10.09 |||1-|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 264

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 11/27 (40%), Positives = 18/27 (66%)
 Frame = +2

Query: 440 INKLQIMCVIEDDKVSVDLLTEKIQEF 520
           INK Q+M   E ++ ++DLL++   EF
Sbjct: 151 INKWQVMTAFELNEENLDLLSQYCSEF 177


>SPBC23G7.08c |rga7||GTPase activating protein
           Rga7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 695

 Score = 25.4 bits (53), Expect = 5.6
 Identities = 11/19 (57%), Positives = 12/19 (63%)
 Frame = +3

Query: 132 PPVLNPRLPPQQRKTTMTT 188
           PPVL P LPP Q  T  T+
Sbjct: 449 PPVLLPTLPPIQTTTIQTS 467


>SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 404

 Score = 25.0 bits (52), Expect = 7.4
 Identities = 23/82 (28%), Positives = 34/82 (41%), Gaps = 8/82 (9%)
 Frame = +3

Query: 63  GIIKLLHTLRLNAKHGLRAPAH*PPVLNPRL--------PPQQRKTTMTTTLIYLVLVTR 218
           GI+     LR+N KH LR   H   V+N  L             K     +L+  V   +
Sbjct: 15  GILLSFEQLRINFKHILRHLEHESHVINSTLTTLISQENASMDEKIEKIDSLLSRVSTVK 74

Query: 219 KRMQKLNEFEKNV*KHMLTRSL 284
           K+M+ L++ E    K   +R L
Sbjct: 75  KKMKHLHDCEALFIKQTKSRLL 96


>SPBC342.02 |||glutaminyl-tRNA synthetase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 811

 Score = 25.0 bits (52), Expect = 7.4
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = +2

Query: 272 DKKSKKPALIAKSSILLDVKPWDDETDMKEME 367
           DK+S  P LIA++ +  ++   D+   +KE E
Sbjct: 692 DKESNSPVLIAETRLFNNLFKCDNPAALKEQE 723


>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1063

 Score = 24.6 bits (51), Expect = 9.8
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = +3

Query: 150 RLPPQQRKTTMTTTLIYLVLVTRKRMQKLNEFE 248
           RLPP+Q K        +L ++ + R Q+LN+ +
Sbjct: 384 RLPPEQLKLIGMLYRKHLQIILQHRQQQLNKIQ 416


>SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit
           Bgs2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1894

 Score = 24.6 bits (51), Expect = 9.8
 Identities = 11/50 (22%), Positives = 24/50 (48%)
 Frame = +2

Query: 260 KAYADKKSKKPALIAKSSILLDVKPWDDETDMKEMENQVRTIEMEGLLWG 409
           KA  +  ++    +    + + ++ W++E    + E QVR + +  L WG
Sbjct: 298 KAETEGTNEPETSVQIDPLNVSMENWENEMKNLDCETQVRQLALYLLCWG 347


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,034,609
Number of Sequences: 5004
Number of extensions: 39304
Number of successful extensions: 106
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 105
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)