Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P16_F_B18
(760 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch... 252 3e-68
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 57 2e-09
SPBC18A7.02c |||seven transmembrane receptor-like protein|Schizo... 29 0.54
SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces pomb... 29 0.95
SPAC824.08 |gda1|gdp1|guanosine-diphosphatase Gda1|Schizosacchar... 28 1.3
SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|ch... 28 1.3
SPAC23C4.02 |crn1||actin binding protein, coronin Crn1|Schizosac... 27 2.9
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 26 6.7
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 6.7
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha... 25 8.9
SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase Gmh1|Schizosa... 25 8.9
>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 538
Score = 252 bits (618), Expect = 3e-68
Identities = 123/207 (59%), Positives = 158/207 (76%), Gaps = 6/207 (2%)
Frame = +2
Query: 158 MADPQGHLLLKIQEQGDLVRKLKAAKESNERVKEEVSKLLALKAQLV--TEDAGPQK--- 322
MAD + +I QG+LVR LK+ S E + +EV+KLL LK + +E +G +K
Sbjct: 1 MADSLVDINERITSQGNLVRSLKSQGASKEDIDKEVAKLLQLKNLKLGGSEVSGKKKDTS 60
Query: 323 FTLKTPKGTRDYNPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKDVLTGKYGEDSKL 502
FTLKTPKGT+D+ + + +R ++ + +F+RHG +DTPVFELK++L+GKYGEDSKL
Sbjct: 61 FTLKTPKGTKDWCDKDVVLREHIFSTVTEIFKRHGGVTLDTPVFELKEILSGKYGEDSKL 120
Query: 503 IYDLKDQGGEILSLRYDLTVPLARYLAMN-KITTLKRYHIAKVYRRDNPAMTRGRYREFY 679
IYDLKDQGGE+ SLRYDLTVP AR+LAMN K+T++KR+HIAKVYRRD PAMT+GR REFY
Sbjct: 121 IYDLKDQGGELCSLRYDLTVPFARWLAMNPKVTSIKRFHIAKVYRRDQPAMTKGRMREFY 180
Query: 680 QCDFDIAGVYDPMVPDAXCLKVVTEIL 760
QCDFDIAG YDPM+PD+ LKV+ E L
Sbjct: 181 QCDFDIAGNYDPMIPDSEALKVLVEAL 207
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 57.2 bits (132), Expect = 2e-09
Identities = 41/125 (32%), Positives = 64/125 (51%), Gaps = 1/125 (0%)
Frame = +2
Query: 389 VLDKIITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPL 568
V D ++ VFRRHGA+ ++ + K + +Y +D + L D+ G +L L YD +P
Sbjct: 990 VRDHVVKVFRRHGAKERESHILFPK---SSQYDKDQASV-SLLDKNGTLLQLPYDTVLPY 1045
Query: 569 ARYLAMNKITTLKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGVYDPM-VPDAXCLKV 745
AR +A N + K Y I+ V+R A GR + + FDI D + DA +K
Sbjct: 1046 ARNVARNAVEEEKTYLISDVFRE---AKGGGRPKAIKEISFDITTNSDNLDWYDAETIKA 1102
Query: 746 VTEIL 760
+ E+L
Sbjct: 1103 LDEVL 1107
>SPBC18A7.02c |||seven transmembrane receptor-like
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 457
Score = 29.5 bits (63), Expect = 0.54
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = -2
Query: 60 FSLVTFIFCFNTNHNPIN 7
FSLV IFC NTN+N N
Sbjct: 371 FSLVALIFCSNTNNNSTN 388
Score = 25.4 bits (53), Expect = 8.9
Identities = 8/24 (33%), Positives = 17/24 (70%)
Frame = -2
Query: 387 LFRIVICCGL*SLVPFGVLRVNFC 316
+FRI++ CG+ ++ F ++ + FC
Sbjct: 356 IFRIMVLCGVVTIFSFSLVALIFC 379
>SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1136
Score = 28.7 bits (61), Expect = 0.95
Identities = 18/78 (23%), Positives = 38/78 (48%), Gaps = 1/78 (1%)
Frame = +2
Query: 377 IRNNVLDKIITVFR-RHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYD 553
++N VL I++ + ++ +E K +L ++ +KL+ L G EILS+ +D
Sbjct: 466 LKNAVLSSIVSTIQIEPNSDLTGYDYYEYKRLLYNEWERFAKLVAYLDHFGDEILSINFD 525
Query: 554 LTVPLARYLAMNKITTLK 607
+ + NK+ ++
Sbjct: 526 PSNAVTYINYANKVAFIR 543
>SPAC824.08 |gda1|gdp1|guanosine-diphosphatase
Gda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 556
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/46 (39%), Positives = 24/46 (52%)
Frame = -2
Query: 576 YRAKGTVKSYLNERISPPWSFKS*INFESSPYFPVSTSFSSKTGVS 439
YR++G V LN P + + F P FPV+TS +KT VS
Sbjct: 57 YRSEGEVVDLLN----PKDELEEPL-FSEEPLFPVTTSIPTKTAVS 97
>SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 404
Score = 28.3 bits (60), Expect = 1.3
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = -1
Query: 418 PKNCNYFIQYIISYCHLLWVIVSCSLWCFKSKL 320
P+N F+ II Y + + ++ CSLW + +KL
Sbjct: 318 PQNLQPFVFMIIQYLYSITTMLPCSLW-YNNKL 349
>SPAC23C4.02 |crn1||actin binding protein, coronin
Crn1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 27.1 bits (57), Expect = 2.9
Identities = 17/71 (23%), Positives = 32/71 (45%)
Frame = +2
Query: 404 ITVFRRHGAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLA 583
+ +FR H A +DT D + G+DSK++ + ++ Y+ P+A
Sbjct: 74 VNLFRGHTAAVLDTDWNPFHDQVLASGGDDSKIMIWKVPEDYTVME-PYEDVHPIAELKG 132
Query: 584 MNKITTLKRYH 616
++ L +YH
Sbjct: 133 HSRKVGLVQYH 143
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex
subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 25.8 bits (54), Expect = 6.7
Identities = 17/48 (35%), Positives = 24/48 (50%), Gaps = 3/48 (6%)
Frame = +2
Query: 626 VYRRDNPAMTRGRYREFYQCDFDIAGVYDPMV---PDAXCLKVVTEIL 760
+Y A++ +YRE +F G+ V PDA CL + TEIL
Sbjct: 245 IYTSPIKALSNQKYRELL-AEFGDVGLMTGDVTINPDATCLVMTTEIL 291
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.8 bits (54), Expect = 6.7
Identities = 17/55 (30%), Positives = 31/55 (56%), Gaps = 2/55 (3%)
Frame = +2
Query: 347 TRDYNPQQMTIRNNVLDKIITVFRRHGAECIDTPVFELKDV--LTGKYGEDSKLI 505
TRDY+ +Q+ R NV+ +++ F + A+ ++ P +K + L G Y E L+
Sbjct: 2511 TRDYHIKQVNNRPNVISTLVSSFVKCAAK-LELPPHLVKYLGKLYGVYHESVSLL 2564
>SPAC13G7.04c |mac1||membrane anchored protein Mac1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 756
Score = 25.4 bits (53), Expect = 8.9
Identities = 15/66 (22%), Positives = 29/66 (43%)
Frame = +2
Query: 425 GAECIDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTL 604
G++ I L+ V + G+ S + + S + D+ PLA ++ + TL
Sbjct: 157 GSDLIAILALCLRSVSISRIGQKSARLEHVDTMNSSYSSYKTDVKYPLALDDKLSSVPTL 216
Query: 605 KRYHIA 622
++H A
Sbjct: 217 PKFHDA 222
>SPAC5H10.11 |gmh1||alpha-1,2-galactosyltransferase
Gmh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 329
Score = 25.4 bits (53), Expect = 8.9
Identities = 8/17 (47%), Positives = 14/17 (82%)
Frame = +1
Query: 151 NHNGRSPGTFIIENSRT 201
+HNG + G+F++ NSR+
Sbjct: 223 DHNGLNAGSFLVRNSRS 239
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,042,948
Number of Sequences: 5004
Number of extensions: 62681
Number of successful extensions: 162
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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