Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P16_F_A13
(816 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 297 2e-81
SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 273 2e-74
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 32 0.085
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.4
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 27 4.2
SPCC569.03 |||DUF1773 family protein 4|Schizosaccharomyces pombe... 27 4.2
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 26 5.6
SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr... 26 7.4
SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 7.4
>SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 439
Score = 297 bits (728), Expect = 2e-81
Identities = 145/239 (60%), Positives = 180/239 (75%), Gaps = 1/239 (0%)
Frame = +2
Query: 101 MVIKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGK 280
M I+ + ARQI+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GK
Sbjct: 1 MAIQKVFARQIYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGK 60
Query: 281 GVLTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXX 460
GVL A+ N+N +IAP + KANL+VT Q+ DE +LKLDGTENKSKLGANAILGVS+
Sbjct: 61 GVLKAVGNVNNIIAPAVVKANLDVTDQKAADEFLLKLDGTENKSKLGANAILGVSMAICR 120
Query: 461 XXXXXXNVPLYKHLADLAG-NNDIVLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFS 637
+PL+K++A+ G VLPVP+FNV+NGGSHAG LA QEFMI PTGA +FS
Sbjct: 121 AGAAQKKLPLWKYIAENFGTKGPYVLPVPSFNVLNGGSHAGGDLAFQEFMILPTGAPSFS 180
Query: 638 EAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKDALYLIQDAIXKAGYAG 814
EAMR G+E YH LK I K+++G + VGDEGG AP++Q ++AL LI +AI KAGY G
Sbjct: 181 EAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPDLQTPQEALDLIVEAINKAGYEG 239
>SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 440
Score = 273 bits (670), Expect = 2e-74
Identities = 131/237 (55%), Positives = 172/237 (72%), Gaps = 1/237 (0%)
Frame = +2
Query: 107 IKSIKARQIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGV 286
I+ I +R I+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV
Sbjct: 4 IQKIYSRSIYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGV 63
Query: 287 LTAIKNINELIAPELTKANLEVTQQREIDELMLKLDGTENKSKLGANAILGVSLXXXXXX 466
A+ N+N +I P L K+++++T QR IDE M+KLDGT +KSKLGAN+I+GVS+
Sbjct: 64 TIAVHNVNNIIGPALVKSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMAVARAA 123
Query: 467 XXXXNVPLYKHLADLAGNNDI-VLPVPAFNVINGGSHAGNKLAMQEFMIFPTGASTFSEA 643
+PLY+++ LAG+ +PVP+FNV+NGG HAG LA QEFMI P A TFSE
Sbjct: 124 AAFLKIPLYEYIGKLAGSKTTECIPVPSFNVLNGGRHAGGDLAFQEFMIMPIKAPTFSEG 183
Query: 644 MRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKDALYLIQDAIXKAGYAG 814
+R GSEVYH LK + K+K+G + VGDEGG AP++ ++AL L+ +AI +AGY G
Sbjct: 184 LRWGSEVYHTLKALAKKKYGASAGNVGDEGGIAPDLTTAEEALDLVNEAIKEAGYDG 240
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 32.3 bits (70), Expect = 0.085
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = -1
Query: 654 PILMASLKVDAPVGKIMNSCMASLFPACDPPLITLKAGTGRTISL 520
PI+ S + + +G+ +N + LFP+CD LI G TI L
Sbjct: 192 PIIQTSAPLGSSLGEFLNKRLPDLFPSCDKFLIVKPVIHGITIFL 236
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 28.3 bits (60), Expect = 1.4
Identities = 22/72 (30%), Positives = 32/72 (44%)
Frame = +2
Query: 137 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINEL 316
D +G +VD ++A +ST LE R N + E V TAI + E
Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198
Query: 317 IAPELTKANLEV 352
ELT+ N+E+
Sbjct: 199 FEGELTEDNIEI 210
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 26.6 bits (56), Expect = 4.2
Identities = 28/114 (24%), Positives = 57/114 (50%), Gaps = 3/114 (2%)
Frame = +2
Query: 77 IKSRSVLKMVIKSIKARQIFDSRGNPTVEVDLV-TELGLFRAAVPSGASTGVHEALELRD 253
IK + ++++ K++ A + ++R + D+V T G + P A+ +H+ E +
Sbjct: 391 IKDQRFIQLIRKALNAGYLTENR----YKYDIVGTPQGSIVS--PILANIYLHQLDEFIE 444
Query: 254 NIKSEYHGKGVLTAIKNINEL--IAPELTKANLEVTQQREIDELMLKLDGTENK 409
N+KSE+ KG + A K +E + + KA E + I ++ +++ NK
Sbjct: 445 NLKSEFDYKGPI-ARKRTSESRHLHYLMAKAKRENADSKTIRKIAIEMRNVPNK 497
>SPCC569.03 |||DUF1773 family protein 4|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 396
Score = 26.6 bits (56), Expect = 4.2
Identities = 31/112 (27%), Positives = 56/112 (50%), Gaps = 3/112 (2%)
Frame = +2
Query: 89 SVLKMVIKSIKARQIFDSRGNPTV-EVDLVTELGLFRAAVPSGASTGVHEALELRDNIKS 265
+V+K I SIK ++ + +G TV + D+ + G A G T + + D++K
Sbjct: 176 AVMKNDIASIKG-EMAEMKGEMTVMKNDIASIKG--EMAEMKGEMTIMKSDI---DSVKG 229
Query: 266 EY-HGKGVLTAIKNINELIAPELTKANLEVTQQRE-IDELMLKLDGTENKSK 415
E KG +T +KN + + E T EVT ++ I +L K+D + +++
Sbjct: 230 EMAEMKGEMTIMKNDIDSVKGETTTLKGEVTAMKDSISQLDRKIDLLDQRTE 281
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 26.2 bits (55), Expect = 5.6
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -3
Query: 409 FVLSAIQLKHEFINLSLLGYFKVGFG 332
F +S+I+ KH++IN+ Y +G G
Sbjct: 113 FSISSIKTKHDYINIIKKHYVSLGVG 138
>SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 527
Score = 25.8 bits (54), Expect = 7.4
Identities = 8/12 (66%), Positives = 10/12 (83%)
Frame = +1
Query: 445 PSCC*GWCCQEK 480
PSCC G CC+E+
Sbjct: 490 PSCCGGHCCKEE 501
>SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 7.4
Identities = 18/77 (23%), Positives = 34/77 (44%)
Frame = +2
Query: 146 GNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINELIAP 325
G P + ++ LG F + S S + ++ + + +E H + K +NE +A
Sbjct: 1109 GEPILTQQVIQNLGGFSSEEVSMVSRCIRSRTQIMNMLATEIHYAASVGQNKYLNEYVA- 1167
Query: 326 ELTKANLEVTQQREIDE 376
L + N E T E+ +
Sbjct: 1168 SLIRTN-EKTHSTELSQ 1183
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,272,018
Number of Sequences: 5004
Number of extensions: 64142
Number of successful extensions: 161
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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