SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P15_pT_N22
         (618 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC576.04 |||bax inhibitor-like protein|Schizosaccharomyces pom...    48   8e-07
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu...    30   0.31 
SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA reductase|Schi...    29   0.54 
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|...    27   2.9  
SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo...    26   5.0  
SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po...    25   8.8  

>SPCC576.04 |||bax inhibitor-like protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 266

 Score = 48.4 bits (110), Expect = 8e-07
 Identities = 26/74 (35%), Positives = 44/74 (59%), Gaps = 1/74 (1%)
 Frame = -1

Query: 399 SMSLMTLVNLFMQSH-FLYQAHLYLGLMLMCGFVLFDTQLIIEKRRMGSKDFVQHALELF 223
           S+ L  L+  F+ S  F+  A    G ++ CG++LFDT  I+   R   ++F+  +L L+
Sbjct: 186 SLILTPLIFFFVPSTPFIDMAFAGFGTLVFCGYILFDTYNIL--HRYSPEEFIMSSLMLY 243

Query: 222 IDFIGMFRRLVIIL 181
           +DFI +F R++ IL
Sbjct: 244 LDFINLFIRILQIL 257


>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1405

 Score = 29.9 bits (64), Expect = 0.31
 Identities = 16/63 (25%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
 Frame = +2

Query: 365 INRLTKVISDIDVN--SVINVPPRNSQLPRSASIAAAEKHTNTRGSAEQSSYYDRWVNYT 538
           ++ +T + S++DV+  S IN   +N  +P S+  +   K + +  + E  S +  W++YT
Sbjct: 279 LSPMTPLSSELDVSAFSEINSKIKNVDVPASSYSSPISKVSPSDVTEEDGSLFFFWMDYT 338

Query: 539 DIF 547
           +++
Sbjct: 339 EMY 341


>SPCC162.09c |hmg1||3-hydroxy-3-methylglutaryl-CoA
           reductase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1053

 Score = 29.1 bits (62), Expect = 0.54
 Identities = 15/55 (27%), Positives = 29/55 (52%)
 Frame = -1

Query: 396 MSLMTLVNLFMQSHFLYQAHLYLGLMLMCGFVLFDTQLIIEKRRMGSKDFVQHAL 232
           +++ +  N  ++  FL+ A +   L+L+  F +    L +E RR  +KD V+  L
Sbjct: 328 LAIFSYCNFGIKQFFLFAAVMIYDLLLLFSFFVAILTLKLEMRRYNAKDDVRKVL 382


>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
            Abc4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1469

 Score = 26.6 bits (56), Expect = 2.9
 Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
 Frame = +3

Query: 480  QIPGVVPSRAVIMIDGSTTLTYSSRGPMLMPDVN--PNPS 593
            Q+P   P    I+ +   +++YS+ GP ++ DVN   NPS
Sbjct: 1201 QVPATWPKEGDIVFN-HVSVSYSAAGPTILKDVNLHINPS 1239


>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
           Itr1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 575

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 10/18 (55%), Positives = 13/18 (72%)
 Frame = +1

Query: 301 YKTAHEHEAKIKMSLVQE 354
           Y TAH +E K K+ L+QE
Sbjct: 291 YPTAHPYEIKTKLYLIQE 308


>SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 361

 Score = 25.0 bits (52), Expect = 8.8
 Identities = 13/53 (24%), Positives = 21/53 (39%)
 Frame = +3

Query: 423 HRGIASCLVXXXXXXXRSIQIPGVVPSRAVIMIDGSTTLTYSSRGPMLMPDVN 581
           HRG++  +               V     +    GST  + SS GP++ P +N
Sbjct: 213 HRGLSPHMAVLKVFVNDKFLTEAVADGLIISTPTGSTAYSLSSGGPIVHPSIN 265


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,343,879
Number of Sequences: 5004
Number of extensions: 44783
Number of successful extensions: 133
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 271646730
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -