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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P15_pT_L11
         (718 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC20G8.07c |erg2||C-8 sterol isomerase Erg2 |Schizosaccharomyc...    33   0.041
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy...    29   0.50 
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo...    26   6.2  
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    25   8.2  

>SPAC20G8.07c |erg2||C-8 sterol isomerase Erg2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 219

 Score = 33.1 bits (72), Expect = 0.041
 Identities = 25/78 (32%), Positives = 43/78 (55%), Gaps = 8/78 (10%)
 Frame = +1

Query: 289 FFSFLPYNLREMSRI*IRRFS--TKFMIINLAVPLI----DRITPRNGECWIRGPSVKYI 450
           F+ F P  L+E+S+  I  ++  TK ++ +L+  L+    D ITP N + W+   +   +
Sbjct: 29  FYQFDPAKLQELSKQSIALYANDTKALLYDLSDRLVAEYGDLITPVNQDEWVHNNAGGAM 88

Query: 451 FAIFVLISVFS--LAFFG 498
             +F+L + FS  L FFG
Sbjct: 89  GTMFILHASFSEYLIFFG 106


>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 874

 Score = 29.5 bits (63), Expect = 0.50
 Identities = 14/44 (31%), Positives = 26/44 (59%)
 Frame = +1

Query: 103 NNLSIFKKECKITSLAQ*IRNVKLINRRFDIFCEATNNVTGRRI 234
           N+  +  +  K  SLA+ +RN  L+++ + + C A N VTG+ +
Sbjct: 290 NHKLVLLESQKDPSLAEHLRNESLLDKGYQLPCFAKNPVTGKAL 333


>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 689

 Score = 25.8 bits (54), Expect = 6.2
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 400 TPRNGECWIRGPSV 441
           +P  GE WIRGP+V
Sbjct: 487 SPPRGEVWIRGPAV 500


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 25.4 bits (53), Expect = 8.2
 Identities = 14/55 (25%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
 Frame = -2

Query: 675 ITDLASDFVSKFR--STSISTEKFEEDFLAAHNDHRQNHGVKPLVLNKKLCKYAE 517
           + D+  D  S  R  +     ++F+ D+ +   + R+N   KP  +  KL K+A+
Sbjct: 678 VDDVFEDMESNLRVPTEDAKIKRFKSDYRSCIYNARRNVFSKPTYMGDKLTKFAK 732


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,972,455
Number of Sequences: 5004
Number of extensions: 64331
Number of successful extensions: 154
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 335201398
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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