BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_pT_I09
(675 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 28 1.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 27 2.5
SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol pyrop... 26 5.7
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 7.6
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 25 7.6
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 25 7.6
SPCC1322.15 |rpl3402|rpl34, rpl34-2|60S ribosomal protein L34|Sc... 25 10.0
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 25 10.0
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 10.0
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 1.4
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -2
Query: 668 PPAPSTRDTPAPXKLSSNITLKPSARPP 585
P A S +TPAP K++S T K +A PP
Sbjct: 152 PAAQSKTETPAP-KVTSESTKKETAAPP 178
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 665 PAPSTRDTPAPXKLSSNITLKPSARPPKPLARFTKPP 555
P S TP+P +L S+ L+P + P P + PP
Sbjct: 216 PTRSPARTPSPIRLYSSDALRPQS-PLSPSVEYLTPP 251
>SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol
pyrophosphate phosphatase fusion 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 25.8 bits (54), Expect = 5.7
Identities = 30/113 (26%), Positives = 50/113 (44%), Gaps = 4/113 (3%)
Frame = -1
Query: 429 YGIAAPYGIAAPYTAYGAYGVAPYGLGVHAW*TDHDREPLFSNISYAKIACFFFNSRYY* 250
YG+ YG+ + ++ + + + + AW + R FS +S+AK A + S +
Sbjct: 468 YGVG--YGMPSSHSQFMGF----FSAYMIAWDYKYRRSQCFSMLSFAKYAIYLTLSTFVC 521
Query: 249 ITLHDRPCQLLNALVIDNVVP-GVGRVW*HRSTVGISRIPG---WRLVLPFLQ 103
+ + L +V ++ GVG W + VG R G W L LP LQ
Sbjct: 522 SSRYLLDFHYLTQVVYGYMIGFGVGLFWVY--LVGKLRSLGVTKWLLSLPPLQ 572
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.4 bits (53), Expect = 7.6
Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 5/45 (11%)
Frame = -2
Query: 671 TPPAPSTRDTPAPXKLSSNITLKPSARPPKPL-----ARFTKPPT 552
+P + R +P+P LS TL P+ PKP RF + PT
Sbjct: 351 SPRSGRPRRSPSPSHLSIPSTLPPADGVPKPTPDGFPRRFERDPT 395
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.4 bits (53), Expect = 7.6
Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
Frame = -2
Query: 668 PPAPSTRDTPAPXKLSSNITLKPSARPPK---PLARFTKP 558
P AP + P P SNI P++ PPK P A +KP
Sbjct: 963 PAAPPSIPPPLPV---SNILSSPTSEPPKDHPPSAPLSKP 999
>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 920
Score = 25.4 bits (53), Expect = 7.6
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = -2
Query: 665 PAPSTRDTPAPXKLSSNITLKPSARPPKPLARFTKPP 555
P +T D PA ++ + +L +P +PLA + P
Sbjct: 252 PKDTTEDNPALERVETTASLDMDYKPLEPLAPIQEAP 288
>SPCC1322.15 |rpl3402|rpl34, rpl34-2|60S ribosomal protein
L34|Schizosaccharomyces pombe|chr 3|||Manual
Length = 111
Score = 25.0 bits (52), Expect = 10.0
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 53 Y*KKLDYR*AINPRRVF*RNGNTKRQPGIREIPTVDRCYQT 175
Y ++L Y N R+ GN R I+++ T+ RC T
Sbjct: 7 YRRRLAYNTRSNKTRIIKTPGNNIRYLHIKKLGTIPRCGDT 47
>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 531
Score = 25.0 bits (52), Expect = 10.0
Identities = 17/69 (24%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = +1
Query: 244 RYSIISAIKKKACYFCIADIRK*RLAVVIGLPSVDAEA-VGGDAVSAISGVGRGDSVRCG 420
R S I K +F + K +L VV ++ E + G + + + +GD ++C
Sbjct: 89 RISSIRYSGSKLAFFDVLYGNK-KLQVVFNKKNIGTEEEMKGKFIPRLKALQKGDCIQCS 147
Query: 421 NAIGGSDQG 447
+G S G
Sbjct: 148 GNVGRSGSG 156
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.0 bits (52), Expect = 10.0
Identities = 15/39 (38%), Positives = 18/39 (46%)
Frame = -2
Query: 671 TPPAPSTRDTPAPXKLSSNITLKPSARPPKPLARFTKPP 555
TPP P TP S+ +L PSA P P+ PP
Sbjct: 414 TPPVP----TPPSLPPSAPPSLPPSAPPSLPMGAPAAPP 448
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,978,907
Number of Sequences: 5004
Number of extensions: 35441
Number of successful extensions: 114
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 114
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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