Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_pT_F03
(748 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa... 91 2e-19
SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc... 91 2e-19
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 28 1.2
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 27 2.1
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 2.8
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 27 2.8
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 26 5.0
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 26 5.0
SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces ... 25 8.7
>SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase
Uch1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 222
Score = 91.1 bits (216), Expect = 2e-19
Identities = 64/209 (30%), Positives = 104/209 (49%), Gaps = 6/209 (2%)
Frame = -1
Query: 748 ESNPDVLNKFXQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKTE 569
E+ P+VL + QK+GV + ++ D+ L+ E ++PRPV +++ +FP S +K +
Sbjct: 6 ENTPEVLEPYLQKIGVQDA-SVFDLFSLE-EIPEYIPRPVHALLFVFPSSGTKTIYKGSR 63
Query: 568 ENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDG-HMQKFLNEAKGL 392
IL K S + + Q I NACGTI L+H+V+N ++++ ++ + A+G
Sbjct: 64 ---ILPKD---SDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAEGS 117
Query: 391 DATARGKLLEKSEGIINAHKELAQEGQTNTPSAED-PVNHHFISFV----HKDGALYELD 227
R KL+E S+ + H A S ED + HFI FV D YELD
Sbjct: 118 SIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYELD 177
Query: 226 GRKAFPVNHGPTSQETLLEDAAKICKEFM 140
GR+ PV H + L + + K ++
Sbjct: 178 GRQEGPVQHSEIESDLLNAEVLSVIKNYI 206
>SPBC409.06 |uch2||ubiquitin C-terminal hydrolase
Uch2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 300
Score = 90.6 bits (215), Expect = 2e-19
Identities = 64/223 (28%), Positives = 112/223 (50%), Gaps = 6/223 (2%)
Frame = -1
Query: 748 ESNPDVLNKFXQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKTE 569
ES+ V + LGV + + ++ LD ++L P + ++ LF + + T
Sbjct: 7 ESDAGVFTDLIENLGVKDV-EVDELYSLDVDSLRQFP-DIYGIIFLFKWNSKVDKPDGTM 64
Query: 568 ENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEAKGLD 389
+ + + NIF+ KQ I+NAC T AL+ + N++D I+L + +F + +K L
Sbjct: 65 DYDSMD-------NIFFAKQVINNACATQALLSVLLNHSDEIDLGTT-LSEFKDFSKTLP 116
Query: 388 ATARGKLLEKSEGIINAHKELAQEG-----QTNTPSAEDPVNHHFISFVHKDGALYELDG 224
+G+ L SE I H A+ + + ED V +HFI++ + + YELDG
Sbjct: 117 PELKGEALGNSEHIRCCHNSFARSDPFISEEVRAATDEDEV-YHFIAYTNINNVFYELDG 175
Query: 223 RKAFPVNHGPTSQETLLEDAAKICKEFMAR-DPNEVRFTVIAL 98
+A P+NHG ++E E A + + +A DP E+RF ++ +
Sbjct: 176 LQAAPINHGSCTKEEFAEKAVSVIQARIANYDPAEIRFNLMVI 218
>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 639
Score = 28.3 bits (60), Expect = 1.2
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = -1
Query: 358 SEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTSQET 179
S ++ ELA + TNT V ++++V + + L K F H P+S E
Sbjct: 126 SRALLEQFAELASK--TNTSHMIHQVYDQYLNYVVLESDFFSLQLPKIFHTFHNPSSDEA 183
Query: 178 LL 173
L+
Sbjct: 184 LI 185
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 27.5 bits (58), Expect = 2.1
Identities = 14/36 (38%), Positives = 17/36 (47%)
Frame = -2
Query: 252 KMEHCMS*MAVKLFLSIMAQLHRKLCWKMQQKFAKN 145
+M HC S L I L R L W+ +QK A N
Sbjct: 508 RMSHCSSNSNKVLASEISESLRRDLLWERRQKAAMN 543
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 27.1 bits (57), Expect = 2.8
Identities = 19/80 (23%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = -1
Query: 349 IINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNH-GPTSQETLL 173
+I+ + E A+E + + + E + H IS ++ ++ D P PT+ E ++
Sbjct: 1205 VISKYIENAKEIELDAVAREGKMVMHVISEHVENAGVHSGDATLVLPPQDLAPTTIERIV 1264
Query: 172 EDAAKICKEFMARDPNEVRF 113
+ AAKI + P ++F
Sbjct: 1265 DAAAKIGEALNITGPYNIQF 1284
>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 934
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/54 (25%), Positives = 23/54 (42%)
Frame = -1
Query: 370 LLEKSEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFP 209
L + +G I K A + + + + ++H S VH+D L DG P
Sbjct: 41 LSDDEDGTIENSKVPASKSKVQKRNESEDISHSLPSIVHEDDKLVGSDGVSTTP 94
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.2 bits (55), Expect = 5.0
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -1
Query: 295 AEDPVNHHFISFVHKDGALYELDGR 221
AE+ V HF+ KD LY++D R
Sbjct: 1099 AEESVKRHFLRLWLKDNGLYDVDIR 1123
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.2 bits (55), Expect = 5.0
Identities = 9/28 (32%), Positives = 20/28 (71%)
Frame = -1
Query: 346 INAHKELAQEGQTNTPSAEDPVNHHFIS 263
++A + L ++ +++T + E+PV+HH S
Sbjct: 337 LSARRALFEKKESSTKNVENPVSHHLKS 364
>SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 296
Score = 25.4 bits (53), Expect = 8.7
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
Frame = -1
Query: 460 NNTDIIELSDGHMQ-KFLNEAKGLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAEDP 284
+NT+++ + ++ K ++ K +D + G L + E +H + GQ +DP
Sbjct: 31 SNTNMLSTEENIVKAKAVSRKKPVDVSYAG--LARHE----SHVDTDYHGQALNTDNQDP 84
Query: 283 VNHHFISFVHKDGALYE 233
N+ SFV K+ LYE
Sbjct: 85 KNYQARSFV-KENELYE 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,033,297
Number of Sequences: 5004
Number of extensions: 62621
Number of successful extensions: 183
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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