Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_pT_E17
(768 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 248 9e-67
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 134 1e-32
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 134 1e-32
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 129 4e-31
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 117 2e-27
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 101 1e-22
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 95 1e-20
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 32 0.10
SPAC4A8.13c |pts1||20S proteasome component beta 5|Schizosacchar... 30 0.32
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 26 6.8
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 248 bits (606), Expect = 9e-67
Identities = 119/225 (52%), Positives = 164/225 (72%), Gaps = 1/225 (0%)
Frame = -2
Query: 761 YDRAITVFSPDGHLLQVXYAQEAVRKGSTAXXXXXXXXXXXXVEKKSVAKLQEXRTVRKI 582
YDRA++VFSPDG LLQV Y QEAVR+G+TA VE+K+V KLQ +KI
Sbjct: 4 YDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQKI 63
Query: 581 CLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSN 402
++D+HV +AFAGL ADARILI++A++E Q+HKL + DPV++EY+TRY+AG++QKYTQS
Sbjct: 64 AMVDNHVCLAFAGLNADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSG 123
Query: 401 GRRPFGISCLIGGFDY-DGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTADEV 225
G RPFG+S LI GFD D +P ++QTEP+GIY WKA A GR++K RE+LEKN+ E
Sbjct: 124 GVRPFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNW--KEG 181
Query: 224 ATENGAVKLAIRALLEVVQSGQKNLEIAVMRRGQPMQMLDLDTIN 90
+ + + LA+ +LLEVVQ+ N+E+A+M G+ ++ L D I+
Sbjct: 182 LSRDETIHLAVSSLLEVVQTASGNIELAIMDPGKDVEFLHSDKID 226
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 134 bits (325), Expect = 1e-32
Identities = 78/214 (36%), Positives = 123/214 (57%), Gaps = 4/214 (1%)
Frame = -2
Query: 764 RYDRAITVFSPDGHLLQVXYAQEAVRKGSTAXXXXXXXXXXXXVEKKSVAKLQEXRTVRK 585
+Y ++T FSP+G L+Q+ YA AV G T+ EKK ++L ++ K
Sbjct: 4 KYAFSLTTFSPNGKLVQIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGASLEK 63
Query: 584 ICLLDDHVVMAFAGLTADARILINRAQIECQS--HKLTVEDPVTLEYITRYIAGLKQKYT 411
+C + + M ++G+ D R+L+++++ Q+ K+ E P T + + + IA + Q+ T
Sbjct: 64 VCAITPDIGMVYSGMGPDFRVLVDKSRKVAQTTYKKIYNEYPPT-KILVQEIASVMQEST 122
Query: 410 QSNGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTAD 231
QS G RPFG+S L+ G D G P L+Q +PSG Y+ WKA A G+S+ + FLEK Y D
Sbjct: 123 QSGGVRPFGVSLLVAGMDEKG-PSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYN-D 180
Query: 230 EVATENGAVKLAIRALLEVVQS--GQKNLEIAVM 135
E+ ++ AV AI AL E + + N+EIAV+
Sbjct: 181 ELELDD-AVHTAILALKETFEGELTEDNIEIAVV 213
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 134 bits (324), Expect = 1e-32
Identities = 75/217 (34%), Positives = 119/217 (54%), Gaps = 8/217 (3%)
Frame = -2
Query: 767 ARYDRAITVFSPDGHLLQVXYAQEAVRKGSTAXXXXXXXXXXXXVEKKSVAKLQEXRTVR 588
+ YDR + FSP+G L QV YA EA++ GSTA VEK+ + L E +V
Sbjct: 6 SEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVE 65
Query: 587 KICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQ 408
K+ +D H+ A +GLTADAR +I A+++ Q+H+ T ++P +E T+ I L ++ +
Sbjct: 66 KLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGE 125
Query: 407 SNG------RRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEK 246
RPFG++ LI G D G P L+ +EPSG Y+ ++A A G ++ + L K
Sbjct: 126 GEDGEERIMSRPFGVALLIAGIDEHG-PQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVK 184
Query: 245 NYTADEVATENGAVKLAIRALLEVVQS--GQKNLEIA 141
+ D E A L ++ L +V++ KN+++A
Sbjct: 185 EFHKDMTLEE--AEVLILKVLRQVMEEKLDSKNVQLA 219
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 129 bits (312), Expect = 4e-31
Identities = 73/200 (36%), Positives = 108/200 (54%), Gaps = 1/200 (0%)
Frame = -2
Query: 761 YDRAITVFSPDGHLLQVXYAQEAVRKGSTAXXXXXXXXXXXXVEKKSVAKL-QEXRTVRK 585
YD T+FSP+G L QV YA EA+ A EKK +KL ++ + K
Sbjct: 5 YDSRTTIFSPEGRLYQVEYALEAINHAGVALGIVAKDGIVLAAEKKVTSKLLEQEESAEK 64
Query: 584 ICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQS 405
+ + D+++ A AGLTADA ILIN A+ Q + T + + E + R + LKQ YTQ
Sbjct: 65 LYHIGDNMLCAVAGLTADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQY 124
Query: 404 NGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTADEV 225
G RPFG+S L G+D+ LFQ+ PSG Y W+AN+ G ++ +V+ + + Y D
Sbjct: 125 GGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQANSIGGNSTSVQSLMRQEYKDDIN 184
Query: 224 ATENGAVKLAIRALLEVVQS 165
E A +A++ L + + S
Sbjct: 185 LDEASA--MAVKFLSKTLDS 202
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 117 bits (281), Expect = 2e-27
Identities = 67/224 (29%), Positives = 115/224 (51%), Gaps = 4/224 (1%)
Frame = -2
Query: 764 RYDRAITVFSPDGHLLQVXYAQEAVRKGSTAXXXXXXXXXXXXVEKKSVAKLQEXRTVRK 585
+YD T +SP G L QV YA EA+++GS K++ +L + +K
Sbjct: 5 QYDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEELSSYQ--KK 62
Query: 584 ICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQS 405
+ +DDH+ +A AGL DAR+L N + E S K P+ + + +A Q TQ
Sbjct: 63 LIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQE 122
Query: 404 NGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTADEV 225
GRRP+G+ L+ G+D G PHL + +PSG+ E+ + G +++ R ++E+N
Sbjct: 123 YGRRPYGVGFLVIGYDESG-PHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERNLDTFPD 181
Query: 224 ATENGAVKLAIRALLEVVQSGQ----KNLEIAVMRRGQPMQMLD 105
++ + A+RAL + + Q +N+ I+V+ + + + D
Sbjct: 182 SSREELILSALRALRDTLSKDQELTEENVSISVIGKDEKYTLYD 225
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 101 bits (242), Expect = 1e-22
Identities = 64/230 (27%), Positives = 111/230 (48%), Gaps = 4/230 (1%)
Frame = -2
Query: 761 YDRAITVFSPDGHLLQVXYAQEAVRK-GSTAXXXXXXXXXXXXVEKKSVAKLQEXRTVRK 585
+DR ITVFSP+G L QV YA +A G T+ +KK KL + TV+
Sbjct: 7 FDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDASTVKH 66
Query: 584 ICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQS 405
+ + + G ADAR ++RA+ E + P+ + + + +A + Q TQ
Sbjct: 67 VFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQR 126
Query: 404 NGRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEKNYTADEV 225
RP G++ + D + P LF+ +P+G Y +KA + G +LEK + D +
Sbjct: 127 AAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKRFKKDGI 186
Query: 224 ATE-NGAVKLAIRALLEVVQSGQKN--LEIAVMRRGQPMQMLDLDTINXY 84
+ V+ I+AL+ + + K+ L+I V+ +P ++L ++ I +
Sbjct: 187 PSNLTDTVETGIQALMSSLSTDFKSTELQIGVVEDDKPFRVLSVEEIEAH 236
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 94.7 bits (225), Expect = 1e-20
Identities = 60/194 (30%), Positives = 94/194 (48%), Gaps = 1/194 (0%)
Frame = -2
Query: 761 YDRAITVFSPDGHLLQVXYAQEAVRKGSTAXXXXXXXXXXXXVEKKSVAKLQEXRTVRKI 582
YD + FSPDG L Q YA +AV ST +EK +KL + R +I
Sbjct: 8 YDLGL-FFSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRI 66
Query: 581 CLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSN 402
+D H+ +A G D + ++ RA+ E S + P+ I + Q +T +
Sbjct: 67 GSVDRHIGIATTGFIPDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYS 126
Query: 401 GRRPFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLEK-NYTADEV 225
RPFG+ + +D +G PHL+ EP+G+Y+ + A G+ + R LEK N+++ ++
Sbjct: 127 SVRPFGVMSFVATYDSEG-PHLYMVEPNGVYWGYNGAAAGKGRQVARNELEKLNFSSLKM 185
Query: 224 ATENGAVKLAIRAL 183
AVK A R L
Sbjct: 186 ---KDAVKEAARIL 196
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 31.9 bits (69), Expect = 0.10
Identities = 25/109 (22%), Positives = 45/109 (41%), Gaps = 2/109 (1%)
Frame = -2
Query: 569 DHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEYITRYIAGLKQKYTQSNGRR- 393
D +V+ +G ADA L+ R Q + E ++ + + L G+R
Sbjct: 52 DDLVIGASGFEADALALVKRIQQRIDLYHDNHERKMSAQSCACMVRTLLY------GKRF 105
Query: 392 -PFGISCLIGGFDYDGSPHLFQTEPSGIYYEWKANATGRSAKTVREFLE 249
P+ + + G D +G ++ +P G Y A G +A + FL+
Sbjct: 106 FPYYVYTTVAGIDKEGKGEIYSFDPVGSYEREWCRAGGSAANFITPFLD 154
>SPAC4A8.13c |pts1||20S proteasome component beta
5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 30.3 bits (65), Expect = 0.32
Identities = 14/72 (19%), Positives = 37/72 (51%)
Frame = -2
Query: 629 KKSVAKLQEXRTVRKICLLDDHVVMAFAGLTADARILINRAQIECQSHKLTVEDPVTLEY 450
+ S L +TV+K+ ++ +++ AG AD + +EC+ H+L ++ +++
Sbjct: 80 RASAGPLIASQTVKKVIEINPYLLGTLAGGAADCQFWETVLGMECRLHQLRNKELISVSA 139
Query: 449 ITRYIAGLKQKY 414
++ ++ + Y
Sbjct: 140 ASKILSNITYSY 151
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.8 bits (54), Expect = 6.8
Identities = 27/87 (31%), Positives = 35/87 (40%), Gaps = 10/87 (11%)
Frame = +1
Query: 349 PS*SNPPIKHEIPNGRRPLL*VYFCLRP--------AMYLVIYS-NVTGSSTVNLCD*HS 501
P+ P +IPN R +L + C P MYL S N T S + L HS
Sbjct: 375 PNFPEPKFFEDIPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEVSVITLAPQHS 434
Query: 502 ICARFINIR-ASAVNPANAITT*SSKR 579
IN+ A A+ IT + KR
Sbjct: 435 FLNIVINLNPALALKSYTIITLLNGKR 461
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,751,386
Number of Sequences: 5004
Number of extensions: 54471
Number of successful extensions: 163
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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