Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_pT_A07
(691 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 69 6e-13
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 64 1e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 60 2e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 42 1e-04
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.013
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.21
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.21
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.63
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 1.9
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.6
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p... 27 2.6
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 4.5
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 68.9 bits (161), Expect = 6e-13
Identities = 31/85 (36%), Positives = 52/85 (61%)
Frame = -3
Query: 659 ISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDE 480
+ E FL + + +F++G + FDKD G I ELR++L++LGEKLS++E
Sbjct: 55 VDMEQFLQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEE 114
Query: 479 VEQLLQGQEDSQGNINYENFVHLIM 405
+++LL+G G +NY +FV +I+
Sbjct: 115 MDELLKGVPVKDGMVNYHDFVQMIL 139
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 64.5 bits (150), Expect = 1e-11
Identities = 34/85 (40%), Positives = 49/85 (57%), Gaps = 1/85 (1%)
Frame = -3
Query: 659 ISFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDE 480
I F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +E
Sbjct: 65 IDFTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEE 122
Query: 479 VEQLL-QGQEDSQGNINYENFVHLI 408
V ++ + D G INYE F +I
Sbjct: 123 VADMIREADTDGDGVINYEEFSRVI 147
Score = 33.9 bits (74), Expect = 0.022
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = -3
Query: 590 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVH 414
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ F+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 413 LI 408
++
Sbjct: 73 MM 74
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 60.5 bits (140), Expect = 2e-10
Identities = 28/73 (38%), Positives = 47/73 (64%), Gaps = 1/73 (1%)
Frame = -3
Query: 620 SKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQ 444
+K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q +
Sbjct: 69 NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNS 128
Query: 443 GNINYENFVHLIM 405
G+ +Y +FV IM
Sbjct: 129 GSFDYYDFVQRIM 141
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 41.5 bits (93), Expect = 1e-04
Identities = 22/62 (35%), Positives = 33/62 (53%)
Frame = -3
Query: 593 NDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVH 414
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE FV
Sbjct: 117 NDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYEKFVD 175
Query: 413 LI 408
I
Sbjct: 176 AI 177
Score = 29.9 bits (64), Expect = 0.36
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = -3
Query: 581 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 462
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.013
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = -3
Query: 572 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 402
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 30.7 bits (66), Expect = 0.21
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = -3
Query: 590 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG-QEDSQGNINYENFVH 414
D E + FD D + I ELR + LG EV ++L+ + +G + E+FV
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVR 97
Query: 413 LIMQ 402
++ +
Sbjct: 98 VMTE 101
Score = 25.4 bits (53), Expect = 7.8
Identities = 14/59 (23%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = -3
Query: 593 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYEN 423
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I+ N
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRN 131
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.21
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = -3
Query: 566 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 411
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.63
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = +3
Query: 474 LYLIITKLLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 644
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 1.9
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = -3
Query: 650 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 483
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 482 EVEQLL 465
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 2.6
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = -3
Query: 542 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 399
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1323
Score = 27.1 bits (57), Expect = 2.6
Identities = 9/29 (31%), Positives = 16/29 (55%)
Frame = -1
Query: 181 VNHN*TCMHLSIRCRLCDXCLNVXPXVKC 95
VNH +C + R+C+ C ++ P + C
Sbjct: 1237 VNHCNSCNSTTSNTRICEKCYSLVPRMSC 1265
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 26.2 bits (55), Expect = 4.5
Identities = 16/67 (23%), Positives = 32/67 (47%)
Frame = +3
Query: 306 LTVSHYGSNCMVTDLVSLQIKVLQQMMT*NSALHDEVNKVLIVDISLRVFLSLQELLYLI 485
L S+Y ++ D V Q+K L Q +S + + + + RV S+ + L+L+
Sbjct: 539 LETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLL 598
Query: 486 ITKLLSE 506
+ ++ E
Sbjct: 599 VNRIKQE 605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,582,919
Number of Sequences: 5004
Number of extensions: 49537
Number of successful extensions: 139
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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