BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_F_N02
(831 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24) 29 4.6
SB_40345| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.6
SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13) 29 4.6
SB_6824| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.1
>SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24)
Length = 315
Score = 29.1 bits (62), Expect = 4.6
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Frame = +3
Query: 354 FAASLADDLGMKHFPEANMDLHYIRPNGVDLRSFD----DQVPEDTRTFDHVNFNQNPNH 521
FAA++ LG+K + M++ VD SFD Q+ E +H+ F++ P H
Sbjct: 32 FAAAIIRTLGIKQGQKGPMEMDLAPDEQVDPNSFDKISSSQLAEQLTFVEHLLFSRVPAH 91
>SB_40345| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1250
Score = 29.1 bits (62), Expect = 4.6
Identities = 10/29 (34%), Positives = 20/29 (68%)
Frame = +3
Query: 303 NSKVIIVEGPVAAGKTAFAASLADDLGMK 389
NS +++V GPV GKT+ +++ +L ++
Sbjct: 440 NSDLVLVTGPVGCGKTSLLSAILGELSVR 468
>SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13)
Length = 899
Score = 29.1 bits (62), Expect = 4.6
Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Frame = +3
Query: 354 FAASLADDLGMKHFPEANMDLHYIRPNGVDLRSFD----DQVPEDTRTFDHVNFNQNPNH 521
FAA++ LG+K + M++ VD SFD Q+ E +H+ F++ P H
Sbjct: 812 FAAAIIRTLGIKQGQKGPMEMDLAPDEQVDPNSFDKISSSQLAEQLTFVEHLLFSRVPAH 871
>SB_6824| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1152
Score = 28.7 bits (61), Expect = 6.1
Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = -2
Query: 617 NSLASIEQVCQSINILTVPGHIHHYLEVCS-QAMVRILIEINMVKGPSVLGYLVVEGPQI 441
NS+ +++++ S N LT ++H L + A L + VKG S LGYL V Q+
Sbjct: 889 NSMTALKRLIVSFNELTRLEDVNHMLFLEHLDASFNKLTTLEGVKGCSRLGYLDVSWNQL 948
Query: 440 DAVRTNI 420
R +
Sbjct: 949 QYTREEL 955
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,068,576
Number of Sequences: 59808
Number of extensions: 490202
Number of successful extensions: 1160
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1080
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1155
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2335516755
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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