Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_F_L18
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 80 4e-16
SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 66 4e-12
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 37 0.003
SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 28 1.2
SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 2.8
SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 2.8
SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 4.9
SPBC16A3.11 |eso1||sister chromatid cohesion protein Eso1|Schizo... 26 4.9
SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 6.4
SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein 3... 25 8.5
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 25 8.5
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 79.8 bits (188), Expect = 4e-16
Identities = 58/200 (29%), Positives = 99/200 (49%), Gaps = 7/200 (3%)
Frame = +3
Query: 159 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN----ESLRGGDLATQARVWQW 326
N D KFP K+P F DG L+E+ AIA+Y+A+ +L G +A+V Q+
Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLASLNKTRALNGTTAEEKAKVLQY 96
Query: 327 ASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERIT 506
S+++SEL A + P + +++Q + A++ + D L ++T+LV R+T
Sbjct: 97 CSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYLVGSRLT 156
Query: 507 LADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAP-PTY- 680
LAD+ L +VL S + ++ R++ T+ HQ ++ A+ L P P
Sbjct: 157 LADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITEPLKFIDQPLPIIK 216
Query: 681 -DPKKYQELXGAQNKKEGKK 737
+ K+ A+ KK+ KK
Sbjct: 217 AENKEAAPAKKAEKKKDEKK 236
>SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 220
Score = 66.5 bits (155), Expect = 4e-12
Identities = 51/206 (24%), Positives = 95/206 (46%), Gaps = 8/206 (3%)
Frame = +3
Query: 39 MAAGVLYTYPENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFES 218
M G LY++ N R L A+ V + + S D KFP K+P F
Sbjct: 1 MFLGTLYSFKTNTRTVCLLELAKLLDLQVDLVETYPH---KFSADLAAKFPLQKLPVFIG 57
Query: 219 ADGKVLLTESNAIAYYVAN------ESLRGGDLATQARVWQWASWSDSELL-PASCA-WV 374
ADG L + Y+ E L + +A + +W + + +++ P + WV
Sbjct: 58 ADGFELSEVIAIVKYFYEKGKHNDKEGLGPVNEVEEAEMLKWMCFINFDIVTPQNVRPWV 117
Query: 375 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQH 554
+ G + + ++ + + + + +LK+ + + RT+LV +R TLAD+ S L F
Sbjct: 118 GMFRGNIPYEEKPFKESATRAIDSLKIPNELVKDRTYLVGDRFTLADLFFGSLLRIFFNS 177
Query: 555 VLDPSVRSSLINVQRWFLTVAHQPQV 632
++D R L ++ R+++T+ HQ ++
Sbjct: 178 IIDEKTRKELPHLTRYYITMFHQAKL 203
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 36.7 bits (81), Expect = 0.003
Identities = 27/95 (28%), Positives = 45/95 (47%), Gaps = 1/95 (1%)
Frame = +3
Query: 456 LDGHLLTRTFLVTER-ITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 632
LD L + TF+ + I++AD+ V++ + H++ L L NV RWF + HQ V
Sbjct: 128 LDEFLKSSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESV 186
Query: 633 SAVVGSLTLCAAPPTYDPKKYQELXGAQNKKEGKK 737
S+++ A + K Q + K+ KK
Sbjct: 187 MEAANSMSMKLANIDLNAPKIQRPSVIKKDKKEKK 221
>SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 442
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/31 (41%), Positives = 17/31 (54%)
Frame = +1
Query: 433 TYWPP*KYWTDIFSHAPSLLPRESHLPMSLS 525
TY PP Y D+FS APS+ + S+S
Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTESSMS 287
>SPAPB1E7.06c |eme1||Holliday junction resolvase subunit
Eme1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 738
Score = 27.1 bits (57), Expect = 2.8
Identities = 16/62 (25%), Positives = 30/62 (48%)
Frame = +3
Query: 66 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 245
P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+
Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611
Query: 246 SN 251
SN
Sbjct: 612 SN 613
>SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 267
Score = 27.1 bits (57), Expect = 2.8
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = +1
Query: 295 IWLPKPVSGSGHHGLTANYCL-LPALGSSLTLV 390
+W+P PV SG T C+ LP G +T V
Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55
>SPBC28F2.09 |||transcription factor TFIIA complex large subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 369
Score = 26.2 bits (55), Expect = 4.9
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = -3
Query: 235 STFPSALSNAGTFPAGNFFKKSSDL 161
+TFP A + GTFP G F S L
Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76
>SPBC16A3.11 |eso1||sister chromatid cohesion protein
Eso1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 26.2 bits (55), Expect = 4.9
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = +3
Query: 246 SNAIAYYVANESLRGGDLATQARVWQWAS 332
S + A Y+ NE+LR G + +R+W AS
Sbjct: 780 STSSAVYIKNENLRKGFVLGISRIWVSAS 808
>SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%)
Frame = +1
Query: 325 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 432
GH L + C P++GSS L+ +C N R+ V++L
Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251
>SPAC11G7.06c |mug132||S. pombe specific UPF0300 family protein
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 25.4 bits (53), Expect = 8.5
Identities = 8/27 (29%), Positives = 19/27 (70%)
Frame = -1
Query: 279 FHWQRSKRWHCFQLGAPFHRHFRMQAL 199
FH ++ +HC+++G P ++++ +AL
Sbjct: 363 FHHDYTE-YHCYRIGKPLYQNYEEEAL 388
>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 568
Score = 25.4 bits (53), Expect = 8.5
Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = +3
Query: 18 LFPKTPTMAAGVLYTYPENFRAYKALIAAQYSG-TDVKVAPNFV-FGE 155
L+ + A G +YT E+FRA K+L S T V+ FV FGE
Sbjct: 321 LYLEAALPALGSVYTIQESFRAEKSLTRRHLSEFTHVEFELPFVNFGE 368
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,249,473
Number of Sequences: 5004
Number of extensions: 69877
Number of successful extensions: 223
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 216
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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