Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P15_F_A07
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 104 1e-23
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 102 6e-23
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 90 3e-19
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 65 1e-11
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 62 1e-10
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.017
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.28
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.85
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.6
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 3.4
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 26 6.0
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 6.0
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 104 bits (250), Expect = 1e-23
Identities = 53/134 (39%), Positives = 82/134 (61%)
Frame = +2
Query: 128 FQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 307
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 308 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDS 487
+ +F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 488 QGNINYENFVHLIM 529
G +NY +FV +I+
Sbjct: 126 DGMVNYHDFVQMIL 139
Score = 27.5 bits (58), Expect = 2.6
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +2
Query: 125 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 238
EF + FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 102 bits (245), Expect = 6e-23
Identities = 55/143 (38%), Positives = 85/143 (59%), Gaps = 3/143 (2%)
Frame = +2
Query: 107 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 280
+++Q+AEF+EAF LFD DG I ++G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 281 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 460
F FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 461 QLL-QGQEDSQGNINYENFVHLI 526
++ + D G INYE F +I
Sbjct: 125 DMIREADTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 90.2 bits (214), Expect = 3e-19
Identities = 50/142 (35%), Positives = 84/142 (59%), Gaps = 1/142 (0%)
Frame = +2
Query: 107 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 286
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 287 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQL 466
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 467 LQ-GQEDSQGNINYENFVHLIM 529
+Q + G+ +Y +FV IM
Sbjct: 120 VQEADPTNSGSFDYYDFVQRIM 141
Score = 35.5 bits (78), Expect = 0.010
Identities = 16/65 (24%), Positives = 35/65 (53%)
Frame = +2
Query: 125 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 304
E+ +AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 305 QAISK 319
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 64.9 bits (151), Expect = 1e-11
Identities = 43/144 (29%), Positives = 68/144 (47%), Gaps = 3/144 (2%)
Frame = +2
Query: 107 SEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--IS 280
+E+Q + EAF+LFDS D I ++ A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 281 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVE 460
E F+ + K D + FD D G IS LR + L E + D E+E
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 461 QLLQGQE-DSQGNINYENFVHLIM 529
+++ + D G IN + F+ ++M
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIMM 173
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 62.1 bits (144), Expect = 1e-10
Identities = 43/143 (30%), Positives = 66/143 (46%)
Frame = +2
Query: 98 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERI 277
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 278 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEV 457
+ FL ++ S ND +E FD +G I + +R LS++G+++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 458 EQLLQGQEDSQGNINYENFVHLI 526
E +L+ S G YE FV I
Sbjct: 156 ESILRSY-TSHGVFYYEKFVDAI 177
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.017
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +2
Query: 362 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 532
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.28
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +2
Query: 368 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 523
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
Score = 27.5 bits (58), Expect = 2.6
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +2
Query: 125 EFQEAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 304
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 305 QAIS 316
IS
Sbjct: 73 IHIS 76
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.85
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -2
Query: 460 LYLIITKLLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 290
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 2.6
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +2
Query: 284 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 451
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 452 EVEQLL 469
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 3.4
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +2
Query: 392 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 535
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 26.2 bits (55), Expect = 6.0
Identities = 16/64 (25%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Frame = -1
Query: 206 LLTHLRSERHVSFHLHEN--RRAGRLLEILPIDPLNIQPYYESTNEIEGNEIGIFKGCDL 33
+ TH+ SE L+E R ++ +LP+ P +++P G + K D+
Sbjct: 216 IFTHISSEDLAHLGLNEQYARPDWMIITVLPVPPPSVRPSISVDGTSRGEDDLTHKLSDI 275
Query: 32 SKSN 21
K+N
Sbjct: 276 IKAN 279
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 26.2 bits (55), Expect = 6.0
Identities = 16/67 (23%), Positives = 32/67 (47%)
Frame = -2
Query: 628 LTVSHYGSNCMVTDLVSLQIKVLQQMMT*NSALHDEVNKVLIVDISLRVFLSLQELLYLI 449
L S+Y ++ D V Q+K L Q +S + + + + RV S+ + L+L+
Sbjct: 539 LETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLL 598
Query: 448 ITKLLSE 428
+ ++ E
Sbjct: 599 VNRIKQE 605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,339,361
Number of Sequences: 5004
Number of extensions: 67157
Number of successful extensions: 232
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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