Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_pT_M21
(742 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 121 9e-29
SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 107 1e-24
SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 77 4e-15
SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 75 1e-14
SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 73 4e-14
SPBC2G2.07c |mug178||mitochondrial ribosomal protein subunit L51... 29 0.92
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 29 0.92
SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 28 1.6
SPBC4B4.06 |vps25||ESCRT II complex subunit Vps25|Schizosaccharo... 26 4.9
SPCC1672.06c |asp1|vip1|inositol hexakisphosphate kinase/inosito... 26 4.9
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 26 6.5
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 25 8.6
>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 121 bits (292), Expect = 9e-29
Identities = 64/170 (37%), Positives = 99/170 (58%)
Frame = -2
Query: 585 GATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSHRPEEKETGAVKVSFDELLTQSDFVI 406
G T+GI+G G IG+ +A+R +AF+ +I+Y NR+ PEE+ GA VSFD+LL +SD +
Sbjct: 156 GKTLGILGLGGIGKTMAKRARAFDM-KIVYHNRTPLPEEEAEGAEFVSFDDLLAKSDVLS 214
Query: 405 CCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRAAGLDVTSP 226
L T+ I K F+KMK + VNT+RG +D+ AL+EAL + +AGLDV
Sbjct: 215 LNLPLNAHTRHIIGKPEFQKMKRGIVIVNTARGAVMDEAALVEALDEGIVYSAGLDVFEE 274
Query: 225 EPLPLDNPLFKLSNCVVLPHIGSATIEARXTMSELTARNILAALHGSDMP 76
EP + L + ++LPH+G+ ++E + M N+ + +P
Sbjct: 275 EP-KIHPGLLENEKVILLPHLGTNSLETQYKMECAVLMNVKNGIVNDSLP 323
>SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 340
Score = 107 bits (258), Expect = 1e-24
Identities = 58/147 (39%), Positives = 85/147 (57%), Gaps = 2/147 (1%)
Frame = -2
Query: 585 GATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSH--RPEEKETGAVKVSFDELLTQSDF 412
G VGI+G G IG++ A+++ E I+Y NR+ EEK GA VSFDELL+ SD
Sbjct: 159 GKRVGIIGMGAIGKSFAQKILPLGCE-IVYHNRNRLEAEEEKRLGASFVSFDELLSSSDV 217
Query: 411 VICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRAAGLDVT 232
+ L P T ++ + + FEKMK+ +NT+RG +++DA I+A+K+ K+ AGLDV
Sbjct: 218 ISINCPLTPATHDLISTKEFEKMKDGVYIINTARGAIINEDAFIKAIKSGKVARAGLDVF 277
Query: 231 SPEPLPLDNPLFKLSNCVVLPHIGSAT 151
EP P + + + PH G T
Sbjct: 278 LNEPTP-NKFWLECDKVTIQPHCGVYT 303
>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 76.6 bits (180), Expect = 4e-15
Identities = 54/165 (32%), Positives = 83/165 (50%), Gaps = 14/165 (8%)
Frame = -2
Query: 591 LAGATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSHRPEEKETGAVKV--SFDELLTQS 418
+ G T+GI+G+G IG ++ +A + Y P G+ K S ELL ++
Sbjct: 193 IRGKTLGIIGYGHIGSQLSVLAEAMGLHVVYYDILPIMP----LGSAKQLSSLPELLHRA 248
Query: 417 DFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRAAGLD 238
DFV PETK + + + F MK + +N SRG VD AL++A K+ KI A +D
Sbjct: 249 DFVSLHVPASPETKNMISSKEFAAMKEGSYLINASRGTVVDIPALVDASKSGKIAGAAID 308
Query: 237 VTSPEPL---------PLDNPLFKLSNC---VVLPHIGSATIEAR 139
V EP L++ +L++C ++ PHIG +T EA+
Sbjct: 309 VYPSEPAGNGKDKFVDSLNSWTSELTHCKNIILTPHIGGSTEEAQ 353
>SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 74.9 bits (176), Expect = 1e-14
Identities = 42/129 (32%), Positives = 72/129 (55%), Gaps = 1/129 (0%)
Frame = -2
Query: 606 MTGPGLAGATVGIVGFGRIGQAVARRVK-AFNTERIIYFNRSHRPEEKETGAVKVSFDEL 430
+ G L G T+G++G GRIG VA+ +K F E + + + ++ EK G V E+
Sbjct: 137 LLGHDLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHDIKPNKELEK-FGIQFVEQQEV 195
Query: 429 LTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRA 250
L ++DF+ L P+T+ + +++ MK +NTSRGG VD AL++A+++ ++
Sbjct: 196 LAKADFLCLHCPLTPDTEHLVDEKLLASMKKGVKIINTSRGGLVDTKALVKAIESGQVGG 255
Query: 249 AGLDVTSPE 223
+DV E
Sbjct: 256 CAMDVYEGE 264
>SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 72.9 bits (171), Expect = 4e-14
Identities = 42/129 (32%), Positives = 66/129 (51%), Gaps = 1/129 (0%)
Frame = -2
Query: 606 MTGPGLAGATVGIVGFGRIGQAVARRVK-AFNTERIIYFNRSHRPEEKETGAVKVSFDEL 430
+ G + G TVG++G G+IG VA+ K F + + Y + E G V +E+
Sbjct: 137 LLGCDIHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYDINPDKKLENY-GVQFVEQNEV 195
Query: 429 LTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRA 250
L ++DF+ L P T I N ++ MK VNTSRGG +D AL++A+ + ++
Sbjct: 196 LKKADFLCLHCPLTPSTTHIVNSDSLALMKKGVTIVNTSRGGLIDTKALVDAIDSGQVGG 255
Query: 249 AGLDVTSPE 223
+DV E
Sbjct: 256 CAIDVYEGE 264
>SPBC2G2.07c |mug178||mitochondrial ribosomal protein subunit
L51-b|Schizosaccharomyces pombe|chr 2|||Manual
Length = 225
Score = 28.7 bits (61), Expect = 0.92
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 269 FSASIKAS*STVPPRLVFTKMAVFFIFSNASLLNISLVS 385
FS+S S T+PP +++ K+ + FS++ +SLVS
Sbjct: 117 FSSSNPKSRPTLPPGILYAKLKLLDSFSDSEKKRLSLVS 155
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 28.7 bits (61), Expect = 0.92
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -2
Query: 576 VGIVGFGRIGQAVARRVKAFNTERIIYFN 490
VGI GFGRIG+ V R T +++ N
Sbjct: 6 VGINGFGRIGRIVLRNALVAKTIQVVAIN 34
>SPAC1296.03c |sxa2||serine carboxypeptidase
Sxa2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 4/35 (11%)
Frame = +2
Query: 353 NASLLNISLVSGTSA----AQQITKSLWVRSSSKL 445
N + + + +VSG +A +QIT S+WV SKL
Sbjct: 220 NINFMGVGIVSGLTADYETQEQITASIWVEHISKL 254
>SPBC4B4.06 |vps25||ESCRT II complex subunit
Vps25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 4.9
Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 4/35 (11%)
Frame = -1
Query: 625 FMGTHLDDRTWTGRCHCWHCWI--W--ENRSGSST 533
F L+D TW + W WI W ENR S T
Sbjct: 12 FFTRQLNDNTWHSQKAAWQMWILLWCRENRQTSIT 46
>SPCC1672.06c |asp1|vip1|inositol hexakisphosphate kinase/inositol
pyrophosphate synthase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 920
Score = 26.2 bits (55), Expect = 4.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3
Query: 279 QSKHLDQQFHHDLYL 323
QSK L +QFH DLY+
Sbjct: 504 QSKDLGEQFHKDLYI 518
>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
1|||Manual
Length = 809
Score = 25.8 bits (54), Expect = 6.5
Identities = 15/53 (28%), Positives = 25/53 (47%)
Frame = +1
Query: 397 RTTNHKVALGKEFIETDFDSTSFLLFRSMTAIKINDSFCVECFHPSCYCLTYS 555
RT AL + + F + FR++ AI S+C+E + S + + YS
Sbjct: 635 RTDRKPSALESSRLSSLFKEFDLVSFRTVPAIHCPYSYCMEITNSSGWKIAYS 687
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.4 bits (53), Expect = 8.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -2
Query: 588 AGATVGIVGFGRIGQAVARRVKAFNTERII 499
+G+TV +VG G +G A + A RII
Sbjct: 193 SGSTVAVVGCGCVGLAAMQGAVAAGASRII 222
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,121,155
Number of Sequences: 5004
Number of extensions: 66017
Number of successful extensions: 206
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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