Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_pT_G15
(745 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 56 4e-09
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 31 0.17
SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 30 0.30
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 30 0.40
SPBC18A7.02c |||seven transmembrane receptor-like protein|Schizo... 27 2.8
SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 26 4.9
SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces ... 26 4.9
SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 26 4.9
SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1 |Schizosa... 26 6.5
SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol pyrop... 26 6.5
SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomy... 26 6.5
SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 26 6.5
SPBC244.01c |sid4||SIN component scaffold protein Sid4 |Schizosa... 25 8.6
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 8.6
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 56.4 bits (130), Expect = 4e-09
Identities = 42/169 (24%), Positives = 77/169 (45%), Gaps = 9/169 (5%)
Frame = -2
Query: 705 DEQLHFLLADMFGAGLDTTSVTLAWFLLYMALFPEEQEEIRKEILXXXXXXXXXXXS--- 535
DE++ A D TS + W +A P+ +++R+E L S
Sbjct: 306 DEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDL 365
Query: 534 --RLPLLMAAICETQRIRSIVPVGIPHGCIEDAY--LGNYRIPKNAMVIPLQWAIHMDPN 367
++ A + E R+R V + +P+ ++ A+ +Y +PK+AMVIP + D
Sbjct: 366 MEKMTYTRAVVKECLRLRPPVLM-VPYR-VKKAFPITPDYTVPKDAMVIPTLYGALHDSK 423
Query: 366 VWEEPEKFKPRRFLAQDGSLLKPQEFIPFQTGKRMCPGDE--LSRMLSC 226
V+ EPE F P R+ + P+ ++ F G +C G ++ +++C
Sbjct: 424 VYPEPETFNPDRWAPNGLAEQSPKNWMVFGNGPHVCLGQRYAVNHLIAC 472
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 31.1 bits (67), Expect = 0.17
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +2
Query: 131 NVTPTVPRISSAVGIFDASRIRCFLNSLLTRPHD 232
NV P V R SSA + I+ F+NSL+ P+D
Sbjct: 963 NVVPLVKRFSSAGNSVNQGSIKKFMNSLIDPPND 996
>SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 495
Score = 30.3 bits (65), Expect = 0.30
Identities = 41/220 (18%), Positives = 85/220 (38%), Gaps = 19/220 (8%)
Frame = -2
Query: 711 MRDEQLHFLLADMFGAGLDTTSVTLAWFLLYMALFPEEQE---EIRKEILXXXXXXXXXX 541
+++ ++ ++ + AG T++ T+ W L + PE E E +K ++
Sbjct: 273 LKEHEIAGMMIALLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGENLELKFDQ 332
Query: 540 XSRLPLLMAAICETQRIRSIVPVGIPHGCIEDAYLGN-YRIPKNAMVIPLQWAIHMDPNV 364
+PLL I ET R+ + + + G+ IP N ++ +
Sbjct: 333 YKDMPLLNYVIQETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLTATEEEY 392
Query: 363 WEEPEKFKPRRF---LAQD----------GSLLK--PQEFIPFQTGKRMCPGDELSRMLS 229
+ F P+R+ + +D G + K ++PF G+ C G++ + M
Sbjct: 393 FTHATDFDPKRWNDRVNEDENAEQIDYGYGLVTKGAASPYLPFGAGRHRCIGEQFAYMHL 452
Query: 228 CGLVSRLFRKQRIRLASKIPTAEEMRGTVGVTLAPPPVKY 109
++S+ L K+P + V + L P + +
Sbjct: 453 STIISKFVHDYTWTLIGKVPNV-DYSSMVALPLGPVKIAW 491
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 29.9 bits (64), Expect = 0.40
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +3
Query: 528 VTLNYQHHRHMDIRIRFPY-VFLPVLLETKPCIAKTKPKSPMSYPVRHRTY 677
V +NY + H I RF + +F+P L + + I P S MS+P + Y
Sbjct: 1452 VEMNYLYAFHFSISNRFVFSLFMPHLKKVEAIIYDKLPGSDMSFPSISKIY 1502
>SPBC18A7.02c |||seven transmembrane receptor-like
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 457
Score = 27.1 bits (57), Expect = 2.8
Identities = 8/22 (36%), Positives = 12/22 (54%)
Frame = -3
Query: 533 GYLCLWQQSVKLRGFDRLFQWE 468
GY C+W S K+ + L W+
Sbjct: 139 GYYCIWSHSKKMLPYQALVNWQ 160
>SPCC1827.04 |||ankyrin repeat protein, unknown biological
role|Schizosaccharomyces pombe|chr 3|||Manual
Length = 600
Score = 26.2 bits (55), Expect = 4.9
Identities = 15/35 (42%), Positives = 20/35 (57%)
Frame = +3
Query: 15 LHTSSPTHCSCTLLSSKAHSNQILVIKLVRNSISP 119
LHTS+P H + T ++K LV KL+RN P
Sbjct: 431 LHTSTPLHYAVTQKNAK------LVAKLLRNGADP 459
>SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 318
Score = 26.2 bits (55), Expect = 4.9
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 623 SKNQAKVTDVVSSPAPNISANRKCNCSSLIY 715
SK A + D+++ APN+SA K NC LI+
Sbjct: 157 SKLNAFLHDLIN--APNVSAEMKENCVRLIF 185
>SPAC144.09c |sfc2||RNA polymerase III transcription factor
TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual
Length = 374
Score = 26.2 bits (55), Expect = 4.9
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = +3
Query: 210 VYLRGHTTTYGTVRHPDTSAYQFETE*ILEA*VDYHLEPKIYA 338
++ R HT H D QF T+ LE ++ H +PK YA
Sbjct: 72 IHKRCHTNVKPFSCHYDGCDAQFYTQQHLERHIEVHRKPKPYA 114
>SPCC297.03 |ssp1||serine/threonine protein kinase Ssp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 652
Score = 25.8 bits (54), Expect = 6.5
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = +2
Query: 515 AISRGNLELSTSSSYGYTDKISLRISSCSSGNKAMYSKNQAKVTDVVSSPAPN 673
A N + + SY D I ++S +G+ YS+ DV S +PN
Sbjct: 541 AFPNDNQDADDACSYNTGDSIP-QVSKSINGHFETYSRTSMDTDDVASFESPN 592
>SPBC530.12c |pdf1||palmitoyl protein thioesterase-dolichol
pyrophosphate phosphatase fusion 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 25.8 bits (54), Expect = 6.5
Identities = 9/23 (39%), Positives = 15/23 (65%)
Frame = -2
Query: 519 MAAICETQRIRSIVPVGIPHGCI 451
+A C+ +IRS++ +G PH I
Sbjct: 115 LAQTCDAAKIRSLITLGSPHSGI 137
>SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 578
Score = 25.8 bits (54), Expect = 6.5
Identities = 14/54 (25%), Positives = 31/54 (57%)
Frame = -3
Query: 317 MVVYLSLKNSFRFKLVSGCVRVTNCPVCCRVAS*VDYSESSVFDSHQKYRQQKR 156
++++ LK + + K++ V +++C +A ++Y + V D H K +QQ+R
Sbjct: 325 LLLFSFLKRNLKKKVI---VFMSSCASVKYMAELLNYIDLPVLDLHGKQKQQRR 375
>SPCC306.11 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 283
Score = 25.8 bits (54), Expect = 6.5
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Frame = +2
Query: 524 RGNLELSTSSSYGYTD---KISLRISSCSSGNKAMYSKNQAKVTDVVSSPAPNISANRKC 694
RG+ + T+SSYG T +I S S+ + + N VT VSS + + + R
Sbjct: 108 RGSACVETNSSYGLTRGLCQIGSSNYSTSANSSNFDNANVINVTTSVSSGSTSTHSKRSS 167
Query: 695 NCSSLIYSAVGSPL 736
S + V P+
Sbjct: 168 GISLSYTNIVDQPI 181
>SPBC244.01c |sid4||SIN component scaffold protein Sid4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 660
Score = 25.4 bits (53), Expect = 8.6
Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = +3
Query: 609 TKPCIAKTKPKSPMS--YPVRHRTYPLIENVTVHLSYTVP 722
T+P +P +S YP +H + P ++N VHL TVP
Sbjct: 221 TRPNQYNPEPNFSLSSGYPQQHFSQPELQNRNVHLE-TVP 259
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.4 bits (53), Expect = 8.6
Identities = 12/39 (30%), Positives = 25/39 (64%), Gaps = 2/39 (5%)
Frame = +2
Query: 500 VSQIAAISRGNLE--LSTSSSYGYTDKISLRISSCSSGN 610
+ +++ + +LE LS S+S GY +ISL++S ++ +
Sbjct: 87 IRKVSRVKSNDLEQLLSVSTSNGYEYRISLQVSETTAAH 125
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,406,266
Number of Sequences: 5004
Number of extensions: 77271
Number of successful extensions: 233
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 220
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 231
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 353266144
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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