Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_pT_E05
(682 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 53 3e-08
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 3e-05
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 41 2e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 38 0.001
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 30 0.36
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 29 0.47
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.47
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 28 1.1
SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosacchar... 28 1.4
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.4
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 1.9
SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr 2... 27 2.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.3
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 4.4
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 26 4.4
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 26 5.8
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 5.8
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 25 7.7
SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 7.7
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 53.2 bits (122), Expect = 3e-08
Identities = 39/136 (28%), Positives = 64/136 (47%), Gaps = 1/136 (0%)
Frame = -2
Query: 660 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSES 481
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 480 -RRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDG 304
V +AFK DK G+G IT++++ V + S S EE D+I E+D
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV--LTSLGERLSQEEVADMIR-------EADT 132
Query: 303 TVDGKVTLEEFMNYYS 256
DG + EEF S
Sbjct: 133 DGDGVINYEEFSRVIS 148
Score = 37.1 bits (82), Expect = 0.002
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = -2
Query: 672 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEF 514
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 43.6 bits (98), Expect = 3e-05
Identities = 28/107 (26%), Positives = 53/107 (49%), Gaps = 6/107 (5%)
Frame = -2
Query: 678 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL-FSQFDTDNSGSISLDEFLIKI 502
R+F +D+DG ++ +EF+ + + NK E + F +D D G IS E + +
Sbjct: 60 RLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
Query: 501 RPPMSESRR-----NIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQT 376
+ + + R IV++ ++DK DG I+ ++ K + S + T
Sbjct: 120 KMMVGTNLREDQLQQIVDKTIMEVDKDRDGKISFEEFKDIVSGSNVT 166
Score = 42.3 bits (95), Expect = 6e-05
Identities = 37/145 (25%), Positives = 68/145 (46%), Gaps = 1/145 (0%)
Frame = -2
Query: 672 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRP- 496
F ++D + S +++ EFL I + A LFS D D G + EF+ +
Sbjct: 30 FIKIDANQSGSIDRNEFL-SIPSVA---SNPLASRLFSVVDEDGGGDVDFQEFINSLSVF 85
Query: 495 PMSESRRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANF 316
+ ++ ++ AFK D DG I+ ++ V + T + ++ + ++ K +
Sbjct: 86 SVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE-DQLQQIVDKTIM--- 141
Query: 315 ESDGTVDGKVTLEEFMNYYSGISVS 241
E D DGK++ EEF + SG +V+
Sbjct: 142 EVDKDRDGKISFEEFKDIVSGSNVT 166
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 40.7 bits (91), Expect = 2e-04
Identities = 37/129 (28%), Positives = 60/129 (46%), Gaps = 6/129 (4%)
Frame = -2
Query: 639 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVE 463
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 462 QAFKKLDKTGDGAITIDD----IKGVYS-VDSQTRYKSGEETEDVIMKRFLANFESDGTV 298
AF+ D +G I+ D+ + +Y V S + E+T + + + + N D
Sbjct: 103 WAFQLYDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNK-IFNM-MDKNK 160
Query: 297 DGKVTLEEF 271
DG++TLEEF
Sbjct: 161 DGQLTLEEF 169
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 38.3 bits (85), Expect = 0.001
Identities = 26/136 (19%), Positives = 59/136 (43%), Gaps = 1/136 (0%)
Frame = -2
Query: 672 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL-IKIRP 496
F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTEK 102
Query: 495 PMSESRRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANF 316
+ +++AF+ D G I++ +++ V + E +D ++ + F
Sbjct: 103 IVERDPLEEIKRAFELFDDDETGKISLRNLRRVAK-------ELNENIDDQELEAMIEEF 155
Query: 315 ESDGTVDGKVTLEEFM 268
+ D DG++ +EF+
Sbjct: 156 DLD--QDGEINEQEFI 169
Score = 37.1 bits (82), Expect = 0.002
Identities = 29/121 (23%), Positives = 53/121 (43%)
Frame = -2
Query: 594 ELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 415
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 414 DDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDGTVDGKVTLEEFMNYYSGISVSID 235
+D V + R + E++ KR F+ D T GK++L ++ +ID
Sbjct: 93 EDFVRVMTEKIVER----DPLEEI--KRAFELFDDDET--GKISLRNLRRVAKELNENID 144
Query: 234 N 232
+
Sbjct: 145 D 145
Score = 33.1 bits (72), Expect = 0.038
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = -2
Query: 678 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL 511
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 29.9 bits (64), Expect = 0.36
Identities = 31/114 (27%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = -2
Query: 597 LELNKSEA--EELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA 424
L LN S EE F + D D+SG +S +EF + + ++R IV+ FK+ +G
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFV--SLLKTRSEIVD-IFKEY-TSGSDK 381
Query: 423 ITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDGTVDGKVTLEEFMNY 262
++++ + S + R S D I +++ +D D K+ L EF ++
Sbjct: 382 MSLEQFRHFLSTSQKARLDS-----DSIRTLYVSFCSND---DSKMGLIEFTSF 427
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 29.5 bits (63), Expect = 0.47
Identities = 21/93 (22%), Positives = 38/93 (40%), Gaps = 2/93 (2%)
Frame = -2
Query: 678 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIR 499
++FR + S +LN+ EF + GL + E LF + G ++ + F +
Sbjct: 491 KVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTEIVM 549
Query: 498 PPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 406
+ + R+ Q DG +T DD+
Sbjct: 550 EELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.5 bits (63), Expect = 0.47
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = -2
Query: 627 EFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 448
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 447 LDKTGDGAITIDDI 406
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 28.3 bits (60), Expect = 1.1
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = -3
Query: 584 KVKPKNSSVNSTQTIVAQSVLMNSLLKSVLLCRNRVVT-L*NKHSRSLTRLVTVQSQL 414
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPAC17G8.05 |med20||mediator complex subunit
Med20|Schizosaccharomyces pombe|chr 1|||Manual
Length = 180
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -2
Query: 390 VDSQTRYKSGEETEDVIMKRFLANFESDGTVDGKV 286
VD T + E ED+I++ L NF T++G +
Sbjct: 55 VDEATMIDAEPELEDIIVRTKLWNFRQSFTIEGSI 89
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 27.9 bits (59), Expect = 1.4
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = -2
Query: 429 GAITIDDIKGVYSVDSQTRYKSGEETEDV 343
G I+I D GVYS + T YKS E+ E +
Sbjct: 126 GVISIHDSTGVYSQITTTPYKSLEDYEQL 154
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 1.9
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = -2
Query: 492 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 403
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 413
Score = 27.1 bits (57), Expect = 2.5
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -2
Query: 411 DIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDG 304
D+ G+ S DS +++ G +DV+ + F NFE+ G
Sbjct: 70 DMYGMNS-DSNSQFDGGVNLDDVLAQMFGMNFEAGG 104
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 3.3
Identities = 20/101 (19%), Positives = 42/101 (41%), Gaps = 3/101 (2%)
Frame = -2
Query: 594 ELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKK---LDKTGDGA 424
+L ++E E +++ D + GS+ DEF + + + N V T +
Sbjct: 853 KLTRTELEHIWNLCDHGDKGSLDRDEFAVALHLIYRKLNGNEVPAVLPPELIPPSTRNFT 912
Query: 423 ITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDGT 301
+++ +K + D+ R G E + + K + S+ T
Sbjct: 913 ESLNQVKNLIKNDTSNRKPFGAENQSKLKKNSFYDNPSETT 953
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 26.2 bits (55), Expect = 4.4
Identities = 21/71 (29%), Positives = 32/71 (45%)
Frame = -2
Query: 624 FLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 445
F+ +E+ LE E FSQ ++ + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 444 DKTGDGAITID 412
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 26.2 bits (55), Expect = 4.4
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = +1
Query: 151 C*SETGKVSSQALELVRLTHH*VKVAVVVNGDADATVVVHELFKSNLPIDCSIGL 315
C + G + +ELVRL + V + V+ + A E+FK DC L
Sbjct: 462 CMDQDGSNAGYDIELVRLVKNSVNIPVIASSGAGIPQHFEEVFKET---DCDAAL 513
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 25.8 bits (54), Expect = 5.8
Identities = 21/84 (25%), Positives = 36/84 (42%), Gaps = 10/84 (11%)
Frame = -2
Query: 546 DNSGSISLDEFLIKIRPPMSESRRNIVEQAFKK--------LDKTGDGAITIDDIKGVYS 391
++ G +S+D FL K++ + + + F+K DK DD+
Sbjct: 853 NSKGKLSIDHFLNKVQSRWHDEEHHYYKTGFRKRVYKYLKIKDKKSKDVDPDDDLVNQLP 912
Query: 390 VDSQT--RYKSGEETEDVIMKRFL 325
+++ T RYKS T I +R L
Sbjct: 913 LNAYTKPRYKSAASTRLNIYQRIL 936
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = +1
Query: 421 DCTVTSLVKLLECLFYNVTTRFRHRRTDFNKEFI 522
D TV + +E LF+N+T +H F + +
Sbjct: 699 DATVANRKDFIEFLFHNITVSSKHTAVIFTSDLL 732
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 25.4 bits (53), Expect = 7.7
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 334 FHYYIFSFFAALISGLRIDRVNTFN 408
FHY F F AA+I + D +NT N
Sbjct: 495 FHYGYFVFTAAVIGHIDPDWINTGN 519
>SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 7.7
Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 5/60 (8%)
Frame = -2
Query: 579 EAEELFSQFDTDNSGSISLDEF-----LIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 415
E ++F D D S +++LDE I I S V+ A KLD+ G G + I
Sbjct: 396 EINDIFHILDNDYSRTVTLDEMEQFTREISIEFRSISSSLRDVDLALGKLDRVGLGVVGI 455
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,522,352
Number of Sequences: 5004
Number of extensions: 48211
Number of successful extensions: 166
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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