Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_pT_A07
(778 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 144 1e-35
SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac... 120 2e-28
SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi... 85 1e-17
SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 76 7e-15
SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 73 5e-14
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.18
SPBC2G2.07c |mug178||mitochondrial ribosomal protein subunit L51... 29 0.98
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 29 0.98
SPAC1296.03c |sxa2||serine carboxypeptidase Sxa2|Schizosaccharom... 28 1.7
SPBC4B4.06 |vps25||ESCRT II complex subunit Vps25|Schizosaccharo... 26 5.2
SPCC1672.06c |asp1|vip1|inositol hexakisphosphate kinase/inosito... 26 5.2
SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr 1|... 26 6.9
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 9.2
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 25 9.2
>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 144 bits (349), Expect = 1e-35
Identities = 81/249 (32%), Positives = 128/249 (51%)
Frame = -3
Query: 770 DTELLDAAGPSLKVVXTISVGHDHIDVAECKKRGVRIGYTPDVXXXXXXXXXXXXXXXTS 591
D E++D PS+K + + G++ +DVA C RG+++ + P
Sbjct: 70 DKEIIDNLPPSVKFICHLGAGYETVDVAACTARGIQVSHVPKAVDDATADVGIFLMLGAL 129
Query: 590 RRVPEAIHEAKTGGWVSWAPTWMTGPGLAGATVGIVGFGRIGQAVARRVKAFNTERIIYF 411
R + I E W + G T+GI+G G IG+ +A+R +AF+ +I+Y
Sbjct: 130 RGFNQGIFELHKNNWNA---NCKPSHDPEGKTLGILGLGGIGKTMAKRARAFDM-KIVYH 185
Query: 410 NRSHRPEEKETGAVKVSFDELLTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTS 231
NR+ PEE+ GA VSFD+LL +SD + L T+ I K F+KMK + VNT+
Sbjct: 186 NRTPLPEEEAEGAEFVSFDDLLAKSDVLSLNLPLNAHTRHIIGKPEFQKMKRGIVIVNTA 245
Query: 230 RGGTVDQDALIEALKTNKIRAAGLDVTSPEPLPLDNPLFKLSNCVVLPHIGSATIEARNT 51
RG +D+ AL+EAL + +AGLDV EP + L + ++LPH+G+ ++E +
Sbjct: 246 RGAVMDEAALVEALDEGIVYSAGLDVFEEEP-KIHPGLLENEKVILLPHLGTNSLETQYK 304
Query: 50 MSELTARNI 24
M N+
Sbjct: 305 MECAVLMNV 313
>SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 340
Score = 120 bits (289), Expect = 2e-28
Identities = 76/234 (32%), Positives = 115/234 (49%), Gaps = 2/234 (0%)
Frame = -3
Query: 764 ELLDAAGPSLKVVXTISVGHDHIDVAECKKRGVRIGYTPDVXXXXXXXXXXXXXXXTSRR 585
E+L P+ K+ T + G++++DV + GV + TP+ T R
Sbjct: 75 EMLGPLLPTCKLFVTGAAGYNNVDVDWATRNGVYVANTPNGPTEGTANMNLMLFMCTLRG 134
Query: 584 VPEAIHEAKTGGWVSWAPTWMTGPGLAGATVGIVGFGRIGQAVARRVKAFNTERIIYFNR 405
EA + G W G VGI+G G IG++ A+++ E I+Y NR
Sbjct: 135 AREAEQSLRLG---KWRQNLSLTDDPYGKRVGIIGMGAIGKSFAQKILPLGCE-IVYHNR 190
Query: 404 SH--RPEEKETGAVKVSFDELLTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTS 231
+ EEK GA VSFDELL+ SD + L P T ++ + + FEKMK+ +NT+
Sbjct: 191 NRLEAEEEKRLGASFVSFDELLSSSDVISINCPLTPATHDLISTKEFEKMKDGVYIINTA 250
Query: 230 RGGTVDQDALIEALKTNKIRAAGLDVTSPEPLPLDNPLFKLSNCVVLPHIGSAT 69
RG +++DA I+A+K+ K+ AGLDV EP P + + + PH G T
Sbjct: 251 RGAIINEDAFIKAIKSGKVARAGLDVFLNEPTP-NKFWLECDKVTIQPHCGVYT 303
>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 466
Score = 85.0 bits (201), Expect = 1e-17
Identities = 69/246 (28%), Positives = 111/246 (45%), Gaps = 15/246 (6%)
Frame = -3
Query: 749 AGPSLKVVXTISVGHDHIDVAECKKRGVRIGYTPDVXXXXXXXXXXXXXXXTSRRVPEAI 570
A SL V+ +G + +D+ +RG+ + +P +R+V +
Sbjct: 116 AADSLIVIGCFCIGTNQVDLDFAAERGIAVFNSPYANSRSVAELVIGYIISLARQVGDRS 175
Query: 569 HEAKTGGWVSWAP-TWMTGPGLAGATVGIVGFGRIGQAVARRVKAFNTERIIYFNRSHRP 393
E G W + W + G T+GI+G+G IG ++ +A + Y P
Sbjct: 176 LELHRGEWNKVSSGCWE----IRGKTLGIIGYGHIGSQLSVLAEAMGLHVVYYDILPIMP 231
Query: 392 EEKETGAVKV--SFDELLTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGT 219
G+ K S ELL ++DFV PETK + + + F MK + +N SRG
Sbjct: 232 ----LGSAKQLSSLPELLHRADFVSLHVPASPETKNMISSKEFAAMKEGSYLINASRGTV 287
Query: 218 VDQDALIEALKTNKIRAAGLDVTSPEPL---------PLDNPLFKLSNC---VVLPHIGS 75
VD AL++A K+ KI A +DV EP L++ +L++C ++ PHIG
Sbjct: 288 VDIPALVDASKSGKIAGAAIDVYPSEPAGNGKDKFVDSLNSWTSELTHCKNIILTPHIGG 347
Query: 74 ATIEAR 57
+T EA+
Sbjct: 348 STEEAQ 353
>SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 75.8 bits (178), Expect = 7e-15
Identities = 53/181 (29%), Positives = 90/181 (49%), Gaps = 14/181 (7%)
Frame = -3
Query: 524 MTGPGLAGATVGIVGFGRIGQAVARRVK-AFNTERIIYFNRSHRPEEKETGAVKVSFDEL 348
+ G L G T+G++G GRIG VA+ +K F E + + + ++ EK G V E+
Sbjct: 137 LLGHDLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHDIKPNKELEK-FGIQFVEQQEV 195
Query: 347 LTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRA 168
L ++DF+ L P+T+ + +++ MK +NTSRGG VD AL++A+++ ++
Sbjct: 196 LAKADFLCLHCPLTPDTEHLVDEKLLASMKKGVKIINTSRGGLVDTKALVKAIESGQVGG 255
Query: 167 AGLDVTSPEP----LPLDNPLFK---------LSNCVVLPHIGSATIEARNTMSELTARN 27
+DV E L N + K N +V H T EA + ++ T +N
Sbjct: 256 CAMDVYEGERRLFYRDLSNEVIKDTTFQQLANFPNVLVTSHQAFFTAEALSAIAHTTLKN 315
Query: 26 I 24
+
Sbjct: 316 V 316
>SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 72.9 bits (171), Expect = 5e-14
Identities = 42/129 (32%), Positives = 66/129 (51%), Gaps = 1/129 (0%)
Frame = -3
Query: 524 MTGPGLAGATVGIVGFGRIGQAVARRVK-AFNTERIIYFNRSHRPEEKETGAVKVSFDEL 348
+ G + G TVG++G G+IG VA+ K F + + Y + E G V +E+
Sbjct: 137 LLGCDIHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYDINPDKKLENY-GVQFVEQNEV 195
Query: 347 LTQSDFVICCAALVPETKEIFNKEAFEKMKNTAIFVNTSRGGTVDQDALIEALKTNKIRA 168
L ++DF+ L P T I N ++ MK VNTSRGG +D AL++A+ + ++
Sbjct: 196 LKKADFLCLHCPLTPSTTHIVNSDSLALMKKGVTIVNTSRGGLIDTKALVDAIDSGQVGG 255
Query: 167 AGLDVTSPE 141
+DV E
Sbjct: 256 CAIDVYEGE 264
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.1 bits (67), Expect = 0.18
Identities = 23/66 (34%), Positives = 35/66 (53%), Gaps = 2/66 (3%)
Frame = +1
Query: 481 PTMPTVAPASP-GPVIQVGAHETQPPVLASCIASGTRRDVAKSR-ARVNSAVASVKTSGV 654
P P APA+P + ++ + + ++AS ASG D+ KSR + +VAS KTS
Sbjct: 477 PPAPAPAPAAPVASIAELPQQDGRANLMASIRASGGM-DLLKSRKVSASPSVASTKTSNP 535
Query: 655 YPIRTP 672
P+ P
Sbjct: 536 -PVEAP 540
>SPBC2G2.07c |mug178||mitochondrial ribosomal protein subunit
L51-b|Schizosaccharomyces pombe|chr 2|||Manual
Length = 225
Score = 28.7 bits (61), Expect = 0.98
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +1
Query: 187 FSASIKAS*STVPPRLVFTKMAVFFIFSNASLLNISLVS 303
FS+S S T+PP +++ K+ + FS++ +SLVS
Sbjct: 117 FSSSNPKSRPTLPPGILYAKLKLLDSFSDSEKKRLSLVS 155
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 28.7 bits (61), Expect = 0.98
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = -3
Query: 494 VGIVGFGRIGQAVARRVKAFNTERIIYFN 408
VGI GFGRIG+ V R T +++ N
Sbjct: 6 VGINGFGRIGRIVLRNALVAKTIQVVAIN 34
>SPAC1296.03c |sxa2||serine carboxypeptidase
Sxa2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 507
Score = 27.9 bits (59), Expect = 1.7
Identities = 14/35 (40%), Positives = 21/35 (60%), Gaps = 4/35 (11%)
Frame = +1
Query: 271 NASLLNISLVSGTSA----AQQITKSLWVRSSSKL 363
N + + + +VSG +A +QIT S+WV SKL
Sbjct: 220 NINFMGVGIVSGLTADYETQEQITASIWVEHISKL 254
>SPBC4B4.06 |vps25||ESCRT II complex subunit
Vps25|Schizosaccharomyces pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 5.2
Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 4/35 (11%)
Frame = -2
Query: 543 FMGTHLDDRTWTGRCHCWHCWI--W--ENRSGSST 451
F L+D TW + W WI W ENR S T
Sbjct: 12 FFTRQLNDNTWHSQKAAWQMWILLWCRENRQTSIT 46
>SPCC1672.06c |asp1|vip1|inositol hexakisphosphate kinase/inositol
pyrophosphate synthase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 920
Score = 26.2 bits (55), Expect = 5.2
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +2
Query: 197 QSKHLDQQFHHDLYL 241
QSK L +QFH DLY+
Sbjct: 504 QSKDLGEQFHKDLYI 518
>SPAC1D4.10 |||tRNA endonuclease|Schizosaccharomyces pombe|chr
1|||Manual
Length = 809
Score = 25.8 bits (54), Expect = 6.9
Identities = 15/53 (28%), Positives = 25/53 (47%)
Frame = +3
Query: 315 RTTNHKVALGKEFIETDFDSTSFLLFRSMTAIKINDSFCVECFHPSCYCLTYS 473
RT AL + + F + FR++ AI S+C+E + S + + YS
Sbjct: 635 RTDRKPSALESSRLSSLFKEFDLVSFRTVPAIHCPYSYCMEITNSSGWKIAYS 687
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.2
Identities = 37/173 (21%), Positives = 74/173 (42%), Gaps = 4/173 (2%)
Frame = +1
Query: 268 SNASLLNISLVSGTSAAQQITKSLWVRSSSKLTLTAPVSFSSGL*LRLK*MILSVL--NA 441
S S+ + S + +SAA T S ++ SSS ++ ++ S SS L + S + +
Sbjct: 185 SQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTS 244
Query: 442 FTLRATA*PILPNPTMPTVAPASPGPVI--QVGAHETQPPVLASCIASGTRRDVAKSRAR 615
+ +T+ + + + T + +S I + + P +S I+S + + +
Sbjct: 245 SSSSSTSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSSPTSTS 304
Query: 616 VNSAVASVKTSGVYPIRTPLFLHSATSI*SWPTEIVXTTFKLGPAASNSSVSS 774
+ +S +S + + S++S S PT T + S+SS SS
Sbjct: 305 STISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSFSS 357
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 506 AGATVGIVGFGRIGQAVARRVKAFNTERII 417
+G+TV +VG G +G A + A RII
Sbjct: 193 SGSTVAVVGCGCVGLAAMQGAVAAGASRII 222
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,353,821
Number of Sequences: 5004
Number of extensions: 72383
Number of successful extensions: 228
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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