SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P14_F_P14
         (735 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      29   0.91 
SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces po...    28   1.6  
SPBC14F5.11c |mug186||sorting nexin Snx41|Schizosaccharomyces po...    28   1.6  
SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schi...    27   2.1  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    26   6.4  
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|...    26   6.4  
SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr 3||...    25   8.5  

>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 28.7 bits (61), Expect = 0.91
 Identities = 16/65 (24%), Positives = 30/65 (46%)
 Frame = -3

Query: 520  IRRLINQSSPSIKKSPLFDPYNFSYSTHGSGRICAGYFPSKFKNKLFM*VGSLIKTSFVF 341
            +R+L      ++ K+    PY   Y+          YFP+  K ++F  +G  +  ++V 
Sbjct: 982  LRKLCQSFLVALSKNIENIPYALCYTAAQLKNSLQRYFPAAHKEEIFGVIGKFVYWAYVA 1041

Query: 340  PVNLS 326
            PV +S
Sbjct: 1042 PVLVS 1046


>SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 374

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +3

Query: 273 NLHKYIDMRHKQLGPIFYERLTGKTKLVFISDPT 374
           +L+KYI + H +L PI YE        +F S+P+
Sbjct: 130 DLNKYIPLSHLKLPPIVYEFDRNVEPAIFPSNPS 163


>SPBC14F5.11c |mug186||sorting nexin Snx41|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 586

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
 Frame = +3

Query: 144 NSNRCSMSTKPHKSLRTIDEMPHKKSLPIIGTKFDL--FSAGGGKNLHKYI-DMRHKQ 308
           N+ + S ST P +     DE   + S+P++GT   L  +  G G+   + + D+RH Q
Sbjct: 402 NNAQTSRSTIPEEDPLFNDEESKEPSVPLMGTDQPLENYHDGNGEQTEECLRDLRHNQ 459


>SPCC1183.04c |pet127||mitochondrial membrane protein
           Pet127|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 524

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 13/29 (44%), Positives = 14/29 (48%)
 Frame = -2

Query: 122 NGRHRRETMHF*IKEDPDISNHFNKPRTQ 36
           NG H+    H   KE   I  HF KPR Q
Sbjct: 453 NGVHKSVVKHLDEKETWSIKYHFRKPRDQ 481


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = +3

Query: 324  YERLTGKTKLVFISDPTHMKSLFLNLEGKYPAHILP 431
            Y  L    KLV+  + T   S+F  ++G Y A ILP
Sbjct: 908  YSALKDPEKLVY--ETTSENSIFFEVDGPYRAMILP 941


>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1369

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
 Frame = +3

Query: 375 HMKSLFLNLEG-KYPAHILPEP--WVLYEKLYGSKRGLFFMDGEDWLINRRIMNKHLLRE 545
           H++SL  N+   + P+H       W  +EKL      + F+ G D    R    +H+L +
Sbjct: 619 HLQSLHPNIYVIRSPSHFRQNALFWAHHEKLVVVDDAITFIGGIDLCFGRYDTPQHILYD 678

Query: 546 DSDV 557
           D  V
Sbjct: 679 DKPV 682


>SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 206

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
 Frame = +3

Query: 111 MSSIRSAVR-SRNSNRCSMSTKPHKSLRTIDEM 206
           +SS  S++  SRN +R  MS KP+K    +DE+
Sbjct: 172 ISSNHSSMHISRNDSRNYMSEKPNKDQSWLDEL 204


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,154,747
Number of Sequences: 5004
Number of extensions: 68254
Number of successful extensions: 156
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -