BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_P14
(735 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 29 0.91
SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces po... 28 1.6
SPBC14F5.11c |mug186||sorting nexin Snx41|Schizosaccharomyces po... 28 1.6
SPCC1183.04c |pet127||mitochondrial membrane protein Pet127|Schi... 27 2.1
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 26 6.4
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 26 6.4
SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 8.5
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 28.7 bits (61), Expect = 0.91
Identities = 16/65 (24%), Positives = 30/65 (46%)
Frame = -3
Query: 520 IRRLINQSSPSIKKSPLFDPYNFSYSTHGSGRICAGYFPSKFKNKLFM*VGSLIKTSFVF 341
+R+L ++ K+ PY Y+ YFP+ K ++F +G + ++V
Sbjct: 982 LRKLCQSFLVALSKNIENIPYALCYTAAQLKNSLQRYFPAAHKEEIFGVIGKFVYWAYVA 1041
Query: 340 PVNLS 326
PV +S
Sbjct: 1042 PVLVS 1046
>SPAC1565.02c |||GTPase activating protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 374
Score = 27.9 bits (59), Expect = 1.6
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +3
Query: 273 NLHKYIDMRHKQLGPIFYERLTGKTKLVFISDPT 374
+L+KYI + H +L PI YE +F S+P+
Sbjct: 130 DLNKYIPLSHLKLPPIVYEFDRNVEPAIFPSNPS 163
>SPBC14F5.11c |mug186||sorting nexin Snx41|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 586
Score = 27.9 bits (59), Expect = 1.6
Identities = 18/58 (31%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +3
Query: 144 NSNRCSMSTKPHKSLRTIDEMPHKKSLPIIGTKFDL--FSAGGGKNLHKYI-DMRHKQ 308
N+ + S ST P + DE + S+P++GT L + G G+ + + D+RH Q
Sbjct: 402 NNAQTSRSTIPEEDPLFNDEESKEPSVPLMGTDQPLENYHDGNGEQTEECLRDLRHNQ 459
>SPCC1183.04c |pet127||mitochondrial membrane protein
Pet127|Schizosaccharomyces pombe|chr 3|||Manual
Length = 524
Score = 27.5 bits (58), Expect = 2.1
Identities = 13/29 (44%), Positives = 14/29 (48%)
Frame = -2
Query: 122 NGRHRRETMHF*IKEDPDISNHFNKPRTQ 36
NG H+ H KE I HF KPR Q
Sbjct: 453 NGVHKSVVKHLDEKETWSIKYHFRKPRDQ 481
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +3
Query: 324 YERLTGKTKLVFISDPTHMKSLFLNLEGKYPAHILP 431
Y L KLV+ + T S+F ++G Y A ILP
Sbjct: 908 YSALKDPEKLVY--ETTSENSIFFEVDGPYRAMILP 941
>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1369
Score = 25.8 bits (54), Expect = 6.4
Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
Frame = +3
Query: 375 HMKSLFLNLEG-KYPAHILPEP--WVLYEKLYGSKRGLFFMDGEDWLINRRIMNKHLLRE 545
H++SL N+ + P+H W +EKL + F+ G D R +H+L +
Sbjct: 619 HLQSLHPNIYVIRSPSHFRQNALFWAHHEKLVVVDDAITFIGGIDLCFGRYDTPQHILYD 678
Query: 546 DSDV 557
D V
Sbjct: 679 DKPV 682
>SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 206
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/33 (42%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +3
Query: 111 MSSIRSAVR-SRNSNRCSMSTKPHKSLRTIDEM 206
+SS S++ SRN +R MS KP+K +DE+
Sbjct: 172 ISSNHSSMHISRNDSRNYMSEKPNKDQSWLDEL 204
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,154,747
Number of Sequences: 5004
Number of extensions: 68254
Number of successful extensions: 156
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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