Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_P02
(755 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 71 2e-13
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 70 3e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 7e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.18
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.7
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.7
SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyce... 25 8.8
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.8
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 25 8.8
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 70.9 bits (166), Expect = 2e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = +1
Query: 106 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 285
AFS++D G G+I ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 286 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 465
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 466 DDGMIPYAAFLKKVMA 513
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 70.1 bits (164), Expect = 3e-13
Identities = 43/135 (31%), Positives = 69/135 (51%), Gaps = 1/135 (0%)
Frame = +1
Query: 106 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 282
AFS++D + G I + LG ++R+L +PT A + FL + ++
Sbjct: 17 AFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMAR 76
Query: 283 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 462
KD D E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 77 KMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132
Query: 463 DDDGMIPYAAFLKKV 507
D DG+I Y F + +
Sbjct: 133 DGDGVINYEEFSRVI 147
Score = 29.9 bits (64), Expect = 0.41
Identities = 18/68 (26%), Positives = 36/68 (52%)
Frame = +1
Query: 316 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 495
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 496 LKKVMAXK 519
L +MA K
Sbjct: 71 L-TMMARK 77
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 55.6 bits (128), Expect = 7e-09
Identities = 36/145 (24%), Positives = 70/145 (48%)
Frame = +1
Query: 79 KNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXX 258
K + AF +YD + G I ++G +LR+L N T A +
Sbjct: 5 KEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFM 64
Query: 259 XFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEV 438
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV +
Sbjct: 65 SFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 439 TKDCMDPEDDDGMIPYAAFLKKVMA 513
++ DP + G Y F++++MA
Sbjct: 120 VQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 31.1 bits (67), Expect = 0.18
Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
Frame = +1
Query: 106 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 282
AF ++D + ID L +RAL N + + F+ + ++
Sbjct: 42 AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101
Query: 283 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 462
++D E+ +L+D +E G + L L E +DD E+ + ++
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157
Query: 463 DDDGMIPYAAFLKKVM 510
D DG I F+ +M
Sbjct: 158 DQDGEINEQEFIAIMM 173
Score = 29.5 bits (63), Expect = 0.54
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +1
Query: 274 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 447
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = +1
Query: 79 KNDVERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 189
++ +E AF ++D + GKI NL + + LN N
Sbjct: 106 RDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.7
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -1
Query: 662 APPEELSPPRALPAPVPQSRASVF*GPSHRT 570
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.7
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +1
Query: 400 LGEKLDDSEVAEVTKDCMDPED 465
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 413
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = +2
Query: 44 PTNTTQNERPQSRTTLKGRLSPSQSTTLKAKARSMPST 157
PTN+T ++ + + R S QSTTL ST
Sbjct: 126 PTNSTTSQASAKHSAVPHRSSQFQSTTLTPSTTDSSST 163
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.4 bits (53), Expect = 8.8
Identities = 19/75 (25%), Positives = 37/75 (49%)
Frame = +2
Query: 83 TTLKGRLSPSQSTTLKAKARSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKS 262
++L +L+P T KAK+ S+ S S S+ + W + V++ A S S++
Sbjct: 348 SSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SPSEA 404
Query: 263 SFPSTAKQRKTKTRE 307
S P+ +K + ++
Sbjct: 405 SLPNLSKYSRKNAKK 419
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 25.4 bits (53), Expect = 8.8
Identities = 22/64 (34%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = +2
Query: 32 TGREPTNTTQNERPQSRTTLKGRLSPS-QSTTLKAKARSMPSTLAIS*ERSTQTPHWQPS 208
T PT T + +RP S T K S +S T K K+ S +ST + + P
Sbjct: 367 TAAAPT-TYEYKRPTSSYTAKDATSKVYRSNTYKPKSSVNGSVYRSKSVKSTTSHNKVPE 425
Query: 209 RNSV 220
RNSV
Sbjct: 426 RNSV 429
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,697,880
Number of Sequences: 5004
Number of extensions: 46811
Number of successful extensions: 157
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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