Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_N18
(739 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione transf... 149 7e-38
AY070254-1|AAL59653.1| 225|Anopheles gambiae glutathione S-tran... 42 2e-05
AF071163-1|AAC79999.1| 218|Anopheles gambiae glutathione S-tran... 39 2e-04
AF071160-4|AAC79992.1| 218|Anopheles gambiae glutathione S-tran... 39 2e-04
AY070256-1|AAL59655.1| 227|Anopheles gambiae glutathione S-tran... 38 4e-04
Z81292-1|CAB03593.1| 209|Anopheles gambiae GSTD1-6 protein prot... 35 0.002
Z71481-1|CAA96105.1| 140|Anopheles gambiae GSTD2 protein protein. 35 0.002
AF071160-1|AAC79995.1| 209|Anopheles gambiae glutathione S-tran... 35 0.002
AF515527-1|AAM61894.1| 211|Anopheles gambiae glutathione S-tran... 34 0.005
AJ439060-3|CAD27754.1| 1645|Anopheles gambiae hypothetical prote... 27 0.80
AJ438610-11|CAD27483.1| 765|Anopheles gambiae hypothetical prot... 27 0.80
AF513634-1|AAM53606.1| 216|Anopheles gambiae glutathione S-tran... 27 0.80
AF316638-1|AAG45166.1| 211|Anopheles gambiae glutathione S-tran... 27 0.80
AF543192-1|AAN40409.1| 636|Anopheles gambiae amino acid transpo... 26 1.4
>AY255856-1|AAP13482.1| 248|Anopheles gambiae glutathione
transferase o1 protein.
Length = 248
Score = 149 bits (362), Expect = 7e-38
Identities = 77/176 (43%), Positives = 108/176 (61%), Gaps = 8/176 (4%)
Frame = +2
Query: 236 KHLQTGDVLP--PYSGKLRVFAMRFCPYAERTVLTLNAKNIPYDLVFINLDQKPEWIFNF 409
KHL G P P GKLR+++MRFCPYA+R L L+AK IPY ++INL +KPEW
Sbjct: 5 KHLAKGSSPPSLPDDGKLRLYSMRFCPYAQRVHLMLDAKKIPYHAIYINLSEKPEWYLEK 64
Query: 410 SPKGTVPALEYEPGK---ALFDSNIINVYLDEKY--PEIPLQASDPLRRAQDKILVESFA 574
+P G VPALE PGK L++S +++ Y++E Y + L +DP +AQD+IL+E FA
Sbjct: 65 NPLGKVPALEI-PGKEGVTLYESLVLSDYIEEAYSAQQRKLYPADPFSKAQDRILIERFA 123
Query: 575 PAQ-SAYYTAAFNAQALEPSMVETYHKGLEGLQKELETRSTKYLHGDEPGWVDYXL 739
+ YY F A + P + + GL+ +KEL+ R T Y GD+PG +DY +
Sbjct: 124 GSVIGPYYRILFAADGIPPGAITEFGAGLDIFEKELKARGTPYFGGDKPGMIDYMI 179
>AY070254-1|AAL59653.1| 225|Anopheles gambiae glutathione
S-transferase E4 protein.
Length = 225
Score = 41.9 bits (94), Expect = 2e-05
Identities = 30/95 (31%), Positives = 50/95 (52%), Gaps = 6/95 (6%)
Frame = +2
Query: 281 LRVFAMRFCPYAERTVLTLNAKNIPYDLVFINL---DQKPEWIFNFSPKGTVPALEYEPG 451
++++ + P LT A + D+V INL + E +P+ T+P ++ + G
Sbjct: 4 IKLYTAKLSPPGRSVELTAKALGLELDIVPINLLAQEHLTEAFRKLNPQHTIPLID-DNG 62
Query: 452 KALFDSNIINVYLDEKY--PE-IPLQASDPLRRAQ 547
++DS+ INVYL KY PE L SD ++RA+
Sbjct: 63 TIVWDSHAINVYLVSKYGKPEGDSLYPSDVVQRAK 97
>AF071163-1|AAC79999.1| 218|Anopheles gambiae glutathione
S-transferase D1-3 protein.
Length = 218
Score = 38.7 bits (86), Expect = 2e-04
Identities = 41/133 (30%), Positives = 59/133 (44%), Gaps = 15/133 (11%)
Frame = +2
Query: 386 KPEWIFNFSPKGTVPALEYEPGKALFDSNIINVYLDEKY----PEIP--LQASDPLRRA- 544
KPE++ +P+ +P L E G L++S I +YL EKY P + L DP RRA
Sbjct: 40 KPEFL-KLNPQHCIPTLVDEDGFVLWESRAIQIYLVEKYCAHDPALAERLYPGDPRRRAV 98
Query: 545 -------QDKILVESFAPAQSAYYTAAFNAQ-ALEPSMVETYHKGLEGLQKELETRSTKY 700
IL + FA YY F + A +P + + + LE L LE
Sbjct: 99 VHQRLFFDVAILYQRFA---EYYYPQIFGKKVAGDPDRLRSMEQALEFLNTFLEGERF-V 154
Query: 701 LHGDEPGWVDYXL 739
GD+P D+ +
Sbjct: 155 AGGDDPTIADFSI 167
>AF071160-4|AAC79992.1| 218|Anopheles gambiae glutathione
S-transferase protein.
Length = 218
Score = 38.7 bits (86), Expect = 2e-04
Identities = 41/133 (30%), Positives = 59/133 (44%), Gaps = 15/133 (11%)
Frame = +2
Query: 386 KPEWIFNFSPKGTVPALEYEPGKALFDSNIINVYLDEKY----PEIP--LQASDPLRRA- 544
KPE++ +P+ +P L E G L++S I +YL EKY P + L DP RRA
Sbjct: 40 KPEFL-KLNPQHCIPTLVDEDGFVLWESRAIQIYLVEKYCAHDPALAERLYPGDPRRRAV 98
Query: 545 -------QDKILVESFAPAQSAYYTAAFNAQ-ALEPSMVETYHKGLEGLQKELETRSTKY 700
IL + FA YY F + A +P + + + LE L LE
Sbjct: 99 VHQRLFFDVAILYQRFA---EYYYPQIFGKKVAGDPDRLRSMEQALEFLNTFLEGERF-V 154
Query: 701 LHGDEPGWVDYXL 739
GD+P D+ +
Sbjct: 155 AGGDDPTIADFSI 167
>AY070256-1|AAL59655.1| 227|Anopheles gambiae glutathione
S-transferase E6 protein.
Length = 227
Score = 37.5 bits (83), Expect = 4e-04
Identities = 39/156 (25%), Positives = 67/156 (42%), Gaps = 8/156 (5%)
Frame = +2
Query: 272 SGKLRVFAMRFCPYAERTVLTLNAKNIPYDLVFINLDQKPEWIFNF---SPKGTVPALEY 442
S K ++ P LT+ A N+ D+ +N+ + F +P T+P L+
Sbjct: 2 SSKPVLYTHTISPAGRAVELTVKALNLDVDVREMNVFKGQHMSDEFKKLNPVQTIPTLD- 60
Query: 443 EPGKALFDSNIINVYLDEKYPEIPLQASDPLRRAQDKILVESFAPAQSAYYTAAF---NA 613
+ G L+DS+ I +YL +Y +D + Q +I F + + F N
Sbjct: 61 DNGFVLWDSHAIMIYLARRYGADSGLYTDEYEQ-QARINAALFFESSILFARLRFCTDNL 119
Query: 614 QALEPSMV--ETYHKGLEGLQKELETRSTKYLHGDE 715
L S + E + LEGLQ+ ++Y+ GD+
Sbjct: 120 TVLGKSAIPEENLQRALEGLQRLERMLQSEYVAGDQ 155
>Z81292-1|CAB03593.1| 209|Anopheles gambiae GSTD1-6 protein
protein.
Length = 209
Score = 35.1 bits (77), Expect = 0.002
Identities = 21/54 (38%), Positives = 32/54 (59%), Gaps = 1/54 (1%)
Frame = +2
Query: 386 KPEWIFNFSPKGTVPALEYEPGKALFDSNIINVYLDEKY-PEIPLQASDPLRRA 544
KPE++ +P+ +P L + G AL++S I +YL EKY + L DP +RA
Sbjct: 40 KPEFL-KLNPQHCIPTL-VDNGFALWESRAIQIYLAEKYGKDDKLYPKDPQKRA 91
>Z71481-1|CAA96105.1| 140|Anopheles gambiae GSTD2 protein protein.
Length = 140
Score = 35.1 bits (77), Expect = 0.002
Identities = 21/54 (38%), Positives = 32/54 (59%), Gaps = 1/54 (1%)
Frame = +2
Query: 386 KPEWIFNFSPKGTVPALEYEPGKALFDSNIINVYLDEKY-PEIPLQASDPLRRA 544
KPE++ +P+ +P L + G AL++S I +YL EKY + L DP +RA
Sbjct: 40 KPEFL-KLNPQHCIPTL-VDNGFALWESRAIQIYLAEKYGKDDKLYPKDPQKRA 91
>AF071160-1|AAC79995.1| 209|Anopheles gambiae glutathione
S-transferase protein.
Length = 209
Score = 35.1 bits (77), Expect = 0.002
Identities = 21/54 (38%), Positives = 32/54 (59%), Gaps = 1/54 (1%)
Frame = +2
Query: 386 KPEWIFNFSPKGTVPALEYEPGKALFDSNIINVYLDEKY-PEIPLQASDPLRRA 544
KPE++ +P+ +P L + G AL++S I +YL EKY + L DP +RA
Sbjct: 40 KPEFL-KLNPQHCIPTL-VDNGFALWESRAIQIYLAEKYGKDDKLYPKDPQKRA 91
>AF515527-1|AAM61894.1| 211|Anopheles gambiae glutathione
S-transferase D10 protein.
Length = 211
Score = 33.9 bits (74), Expect = 0.005
Identities = 22/64 (34%), Positives = 36/64 (56%), Gaps = 3/64 (4%)
Frame = +2
Query: 320 RTVLTLNAK-NIPYDLVFIN--LDQKPEWIFNFSPKGTVPALEYEPGKALFDSNIINVYL 490
++VL + K I +DL +N L + E + F+P+ T+P E G +++S I +YL
Sbjct: 13 QSVLLVGKKLGITFDLKEVNPHLPEVREQLRKFNPQHTIPTF-IEDGHVIWESYAIAIYL 71
Query: 491 DEKY 502
EKY
Sbjct: 72 VEKY 75
>AJ439060-3|CAD27754.1| 1645|Anopheles gambiae hypothetical protein
protein.
Length = 1645
Score = 26.6 bits (56), Expect = 0.80
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +2
Query: 125 SPLTSIISIKSHQKATLHVDTNLF 196
S L + + IK H HVDT LF
Sbjct: 18 SDLDTFVQIKQHYTTKYHVDTGLF 41
>AJ438610-11|CAD27483.1| 765|Anopheles gambiae hypothetical protein
protein.
Length = 765
Score = 26.6 bits (56), Expect = 0.80
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +2
Query: 125 SPLTSIISIKSHQKATLHVDTNLF 196
S L + + IK H HVDT LF
Sbjct: 18 SDLDTFVQIKQHYTTKYHVDTGLF 41
>AF513634-1|AAM53606.1| 216|Anopheles gambiae glutathione
S-transferase D5 protein.
Length = 216
Score = 26.6 bits (56), Expect = 0.80
Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +2
Query: 410 SPKGTVPALEYEPGKALFDSNIINVYLDEKY-PEIPLQASDPLRRA 544
+P+ +P + G +++S I +YL EKY + L DP R+
Sbjct: 46 NPQHLIPTFVEDDGHVIWESYAIAIYLVEKYGQDDALYPKDPKVRS 91
>AF316638-1|AAG45166.1| 211|Anopheles gambiae glutathione
S-transferase D12 protein.
Length = 211
Score = 26.6 bits (56), Expect = 0.80
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = +2
Query: 410 SPKGTVPALEYEPGKALFDSNIINVYLDEKY 502
+P+ T+P L + G L++S I +YL EKY
Sbjct: 46 NPQHTIPTL-VDNGHILWESYAILIYLAEKY 75
>AF543192-1|AAN40409.1| 636|Anopheles gambiae amino acid
transporter Ag_AAT8 protein.
Length = 636
Score = 25.8 bits (54), Expect = 1.4
Identities = 17/49 (34%), Positives = 22/49 (44%)
Frame = +2
Query: 272 SGKLRVFAMRFCPYAERTVLTLNAKNIPYDLVFINLDQKPEWIFNFSPK 418
SGK F F PY VL + A +P + I KP+W + PK
Sbjct: 278 SGKASYFLALF-PYVVMAVLLVRACTLPGAVDGIVYFLKPQWDKIYDPK 325
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 722,404
Number of Sequences: 2352
Number of extensions: 14622
Number of successful extensions: 36
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 33
length of database: 563,979
effective HSP length: 63
effective length of database: 415,803
effective search space used: 75676146
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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