Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_N02
(769 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 53 4e-08
SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 46 5e-06
SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 44 2e-05
SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces po... 44 2e-05
SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces pom... 44 2e-05
SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces pom... 40 4e-04
SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces pomb... 34 0.019
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 27 2.2
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 25 9.0
SPBC36.10 |||mitochondrial intermembrane space protein sorting p... 25 9.0
>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 53.2 bits (122), Expect = 4e-08
Identities = 34/134 (25%), Positives = 62/134 (46%), Gaps = 1/134 (0%)
Frame = +2
Query: 326 ALPCIVDVRDEKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTR 505
AL DV Q++ A+++ ++ ID+L+NNA T Q + +D ++ R
Sbjct: 47 ALALQADVSKADQIETAIEKVIQTLGPIDVLINNAGLADDTPFEQLSHESWDHDVSLVLR 106
Query: 506 GTFLASKICLP-VLKNSNHAHILNLSPPLNMNPYWFSIHVAYTMAKYGMSMCVLGMSEEF 682
G +L + +P + K I+N+ ++N + + AY+ AK G+ ++ +
Sbjct: 107 GNYLTQRYVIPHMAKQGKGGSIVNIG---SVNGHIYLGSPAYSAAKAGLENLTKALAVRY 163
Query: 683 KPFNIGVNALWPKT 724
P I VN P T
Sbjct: 164 GPLGIRVNVCAPGT 177
>SPAC521.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 259
Score = 46.4 bits (105), Expect = 5e-06
Identities = 38/148 (25%), Positives = 64/148 (43%), Gaps = 3/148 (2%)
Frame = +2
Query: 143 KLAGRTLFITGASRXXXXXXXXXXXXXXX-NVVIAAKTAEPHPKLPGTIYTAAEEIEA-L 316
+L G+T+ ITGAS +++AA+ T+ A+E+E+
Sbjct: 3 RLDGKTILITGASSGIGKSTAFEIAKVAKVKLILAARRFS-------TVEEIAKELESKY 55
Query: 317 GGKALPCIVDVRDEKQVQKAVDEAVKKFNGIDILVNNAS-AISLTGTAQTDMKRYDLMHN 493
LP +DV D K + ++ K+F ID+L+NNA A+ ++ M
Sbjct: 56 EVSVLPLKLDVSDLKSIPGVIESLPKEFADIDVLINNAGLALGTDKVIDLNIDDAVTMIT 115
Query: 494 INTRGTFLASKICLPVLKNSNHAHILNL 577
N G ++ LP+ + N ILN+
Sbjct: 116 TNVLGMMAMTRAVLPIFYSKNKGDILNV 143
>SPAC977.08 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 236
Score = 44.4 bits (100), Expect = 2e-05
Identities = 21/80 (26%), Positives = 42/80 (52%)
Frame = +2
Query: 341 VDVRDEKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTRGTFLA 520
+DV + ++K+V++A+ F ID+L+NNA + T ++ + N N G
Sbjct: 21 LDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTNVFGALEV 80
Query: 521 SKICLPVLKNSNHAHILNLS 580
K P+ ++ ++ I+N+S
Sbjct: 81 IKAITPIFRSQHNGMIINVS 100
>SPBC1348.09 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 236
Score = 44.4 bits (100), Expect = 2e-05
Identities = 21/80 (26%), Positives = 42/80 (52%)
Frame = +2
Query: 341 VDVRDEKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTRGTFLA 520
+DV + ++K+V++A+ F ID+L+NNA + T ++ + N N G
Sbjct: 21 LDVNNFSSIKKSVEKAISHFGRIDVLLNNAGYSVYSPLESTTEEQIHNIFNTNVFGALEV 80
Query: 521 SKICLPVLKNSNHAHILNLS 580
K P+ ++ ++ I+N+S
Sbjct: 81 IKAITPIFRSQHNGMIINVS 100
>SPCC162.03 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 292
Score = 44.0 bits (99), Expect = 2e-05
Identities = 39/145 (26%), Positives = 66/145 (45%), Gaps = 3/145 (2%)
Frame = +2
Query: 293 AAEEIEALGGKALPCIVDVRDEKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQT-DM 469
A + I K L +DV D K V+ A +A ++F +DI++NNA L G ++ ++
Sbjct: 38 APDTITIEHSKLLKLKLDVTDVKSVETAFKDAKRRFGNVDIVINNA-GYGLVGEFESYNI 96
Query: 470 KRYDLMHNINTRGTFLASKICLPVLKNSNH-AHILNLSPPLNMNPY-WFSIHVAYTMAKY 643
+ N+N G +K L +++ S IL +S P S++ A A
Sbjct: 97 EEMHRQMNVNFWGVAYITKEALNLMRESGKGGRILQISSVAGYYPSPCLSMYNASKFAVE 156
Query: 644 GMSMCVLGMSEEFKPFNIGVNALWP 718
G+S + M E +NI + + P
Sbjct: 157 GLSQTI--MRELDPNWNIAITIVQP 179
>SPAC4H3.08 |||short chain dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 286
Score = 39.9 bits (89), Expect = 4e-04
Identities = 33/116 (28%), Positives = 57/116 (49%), Gaps = 2/116 (1%)
Frame = +2
Query: 377 VDEAVKKFNGIDILVNNASAISLTGTAQ-TDMKRYDLMHNINTRGTFLASKICLPVLKNS 553
VD A+KK ID+LVNN + + + + D +++DL N F +K + +K+
Sbjct: 112 VDFALKKLGWIDVLVNNIAYQQVAQSIEDIDDEQWDLTFKTNIFSFFWVTKAAISHMKSG 171
Query: 554 NHAHILNLSPPLNMNPYWFSIHVA-YTMAKYGMSMCVLGMSEEFKPFNIGVNALWP 718
+ I+N S ++N Y + YT K ++ G+S ++ I VNA+ P
Sbjct: 172 --SSIVNCS---SINAYVGRPDLLDYTSTKGAITAFTRGLSNQYAQHGIRVNAVAP 222
>SPCC736.13 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 339
Score = 34.3 bits (75), Expect = 0.019
Identities = 31/136 (22%), Positives = 55/136 (40%)
Frame = +2
Query: 146 LAGRTLFITGASRXXXXXXXXXXXXXXXNVVIAAKTAEPHPKLPGTIYTAAEEIEALGGK 325
L G+ +TG+S V +A + E + K+ I+ E K
Sbjct: 40 LTGKVALVTGSSGGIGYVTALELARKGAKVYLAGRNEEKYQKVMKQIHD-----EVRHSK 94
Query: 326 ALPCIVDVRDEKQVQKAVDEAVKKFNGIDILVNNASAISLTGTAQTDMKRYDLMHNINTR 505
+D+ D + V +A + + K + ILVNNA ++ D Y+L N
Sbjct: 95 IRFLRLDLLDFESVYQAAESFIAKEEKLHILVNNAGIMNPPFELTKD--GYELQIQTNYL 152
Query: 506 GTFLASKICLPVLKNS 553
+L +++ LP L+ +
Sbjct: 153 SHYLFTELLLPTLRRT 168
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = -2
Query: 714 HRALTPILNGLNSSLMPSTHIDIPYL-AIVYAT*IENQYGFMLRGGD 577
HR L ILN N S+ ID+P A+ + T +E + + G D
Sbjct: 624 HRNLKRILNNRNKSISSGEGIDMPTAEALAFGTLLEEGHHVRVSGQD 670
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 25.4 bits (53), Expect = 9.0
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 4/43 (9%)
Frame = -2
Query: 711 RALT-PILNGLNSSL---MPSTHIDIPYLAIVYAT*IENQYGF 595
R LT P+ +G+ + + HI +P+ A+ T IEN+Y F
Sbjct: 397 RNLTIPLASGMTTRFTYTISGKHIQVPWNALHSTTKIENEYTF 439
>SPBC36.10 |||mitochondrial intermembrane space protein sorting
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.4 bits (53), Expect = 9.0
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +2
Query: 251 TAEPHPKLPGTIYTAAEEIEAL 316
T PHP+L T++ IEAL
Sbjct: 110 TYSPHPELEATVFQQEARIEAL 131
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,953,433
Number of Sequences: 5004
Number of extensions: 59645
Number of successful extensions: 158
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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