Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_L20
(666 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr... 195 4e-51
SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|... 188 6e-49
SPAC26H5.04 |||vacuolar import and degradation protein Vid28|Sch... 34 0.021
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 29 0.80
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 28 1.4
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 27 2.4
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 25 7.4
SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 25 9.8
SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 25 9.8
>SPBC1604.08c |imp1||importin alpha|Schizosaccharomyces pombe|chr
2|||Manual
Length = 539
Score = 195 bits (476), Expect = 4e-51
Identities = 98/201 (48%), Positives = 136/201 (67%), Gaps = 9/201 (4%)
Frame = +1
Query: 91 IPLLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWF 270
+ LLS V +QT ALR+VGNIVTGTD QTQ++++C AL+ FP+LL+H KE I KEA W
Sbjct: 296 VELLSSPSVNIQTPALRSVGNIVTGTDAQTQIIIDCGALNAFPSLLSHQKENIRKEACWT 355
Query: 271 LSNITAGNKQQVQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSIS--GTRDQVAAL 444
+SNITAGN QQ+QA+I++ L+P +V LS +++T+KEA WA+SN + G DQ+ L
Sbjct: 356 ISNITAGNTQQIQAIIESNLIPPLVHLLSYADYKTKKEACWAISNATSGGLGQPDQIRYL 415
Query: 445 INCGVIPPFCNLLSCKDTQVINVVLDGLSNMLK-------MAGDNAEQVANMIEECGGID 603
++ GVI P C++L+ D ++I V LD + N+LK M +N Q A +EE GG+D
Sbjct: 416 VSQGVIKPLCDMLNGSDNKIIQVALDAIENILKVGEMDRTMDLENINQYAVYVEEAGGMD 475
Query: 604 KIEDLQTHXKMEIYKMAYDII 666
I DLQ+ +IY AY II
Sbjct: 476 MIHDLQSSGNNDIYLKAYSII 496
Score = 76.2 bits (179), Expect = 4e-15
Identities = 51/190 (26%), Positives = 88/190 (46%)
Frame = +1
Query: 97 LLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWFLS 276
LL ++V++ A A+ + G +E+ +L+ LL+ P I A+ +
Sbjct: 256 LLYSEDVEIIVDACWAISYLSDGPNEKIGAILDVGCAPRLVELLSSPSVNIQTPALRSVG 315
Query: 277 NITAGNKQQVQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSISGTRDQVAALINCG 456
NI G Q Q +ID G L + LS + +KEA W +SN++ +G Q+ A+I
Sbjct: 316 NIVTGTDAQTQIIIDCGALNAFPSLLSHQKENIRKEACWTISNIT-AGNTQQIQAIIESN 374
Query: 457 VIPPFCNLLSCKDTQVINVVLDGLSNMLKMAGDNAEQVANMIEECGGIDKIEDLQTHXKM 636
+IPP +LLS D + +SN +Q+ ++ + G I + D+
Sbjct: 375 LIPPLVHLLSYADYKTKKEACWAISNATSGGLGQPDQIRYLVSQ-GVIKPLCDMLNGSDN 433
Query: 637 EIYKMAYDII 666
+I ++A D I
Sbjct: 434 KIIQVALDAI 443
Score = 59.7 bits (138), Expect = 4e-10
Identities = 36/123 (29%), Positives = 59/123 (47%), Gaps = 4/123 (3%)
Frame = +1
Query: 187 VLNCDALSHFPALLTHPKEKICKEAVWFLSNITAGNKQQVQAVIDAGLLPMIVANLSKGE 366
V+ C + F L + EA W L+NI +G Q + V+D+G +P + LS E
Sbjct: 116 VIACGVVDRFVQFLESEHHLLQFEAAWALTNIASGTTDQTRIVVDSGAVPRFIQLLSSPE 175
Query: 367 FQTQKEAAWAVSNLS--ISGTRDQVAALINCGVIPPFCNLL--SCKDTQVINVVLDGLSN 534
+++ WA+ N++ S RD V + GV+ P N+L S D ++ LSN
Sbjct: 176 KDVREQVVWALGNIAGDSSACRDYV---LGNGVLQPLLNILQSSASDVSMLRNATWTLSN 232
Query: 535 MLK 543
+ +
Sbjct: 233 LCR 235
Score = 57.6 bits (133), Expect = 2e-09
Identities = 38/141 (26%), Positives = 69/141 (48%), Gaps = 2/141 (1%)
Frame = +1
Query: 91 IPLLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWF 270
+ L + +Q A A+ NI +GT +QT++V++ A+ F LL+ P++ + ++ VW
Sbjct: 126 VQFLESEHHLLQFEAAWALTNIASGTTDQTRIVVDSGAVPRFIQLLSSPEKDVREQVVWA 185
Query: 271 LSNITAGNKQQVQAVIDAGLL-PMI-VANLSKGEFQTQKEAAWAVSNLSISGTRDQVAAL 444
L NI + V+ G+L P++ + S + + A W +SNL +
Sbjct: 186 LGNIAGDSSACRDYVLGNGVLQPLLNILQSSASDVSMLRNATWTLSNLCRGKNPPPNWST 245
Query: 445 INCGVIPPFCNLLSCKDTQVI 507
I+ V P LL +D ++I
Sbjct: 246 ISVAV-PILAKLLYSEDVEII 265
Score = 49.6 bits (113), Expect = 4e-07
Identities = 30/86 (34%), Positives = 46/86 (53%)
Frame = +1
Query: 304 VQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSISGTRDQVAALINCGVIPPFCNLL 483
+ VI G++ V L Q EAAWA++N++ SGT DQ +++ G +P F LL
Sbjct: 113 IDQVIACGVVDRFVQFLESEHHLLQFEAAWALTNIA-SGTTDQTRIVVDSGAVPRFIQLL 171
Query: 484 SCKDTQVINVVLDGLSNMLKMAGDNA 561
S + V V+ L N +AGD++
Sbjct: 172 SSPEKDVREQVVWALGN---IAGDSS 194
Score = 30.7 bits (66), Expect = 0.20
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 3 DTVWAISYLTXGGNEQIQMVIXSGIVTTLXSVV 101
D WAISYL+ G NE+I ++ G L ++
Sbjct: 267 DACWAISYLSDGPNEKIGAILDVGCAPRLVELL 299
Score = 29.1 bits (62), Expect = 0.60
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +3
Query: 3 DTVWAISYLTXGGNEQIQMVIXSGIVTTL 89
+ W IS +T G +QIQ +I S ++ L
Sbjct: 351 EACWTISNITAGNTQQIQAIIESNLIPPL 379
>SPCC962.03c |cut15||karyopherin Cut15|Schizosaccharomyces pombe|chr
3|||Manual
Length = 542
Score = 188 bits (458), Expect = 6e-49
Identities = 92/201 (45%), Positives = 134/201 (66%), Gaps = 9/201 (4%)
Frame = +1
Query: 91 IPLLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWF 270
+ LL H +VQT ALR+VGNIVTG D QTQV++NC ALS +LL+ P++ + KEA W
Sbjct: 296 VELLMHPSAQVQTPALRSVGNIVTGDDVQTQVIINCGALSALLSLLSSPRDGVRKEACWT 355
Query: 271 LSNITAGNKQQVQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSISGTR--DQVAAL 444
+SNITAGN Q+Q VI+A ++P ++ L+ +F+ QKEA WA+SN + G R DQ+ L
Sbjct: 356 ISNITAGNSSQIQYVIEANIIPPLIHLLTTADFKIQKEACWAISNATSGGARRPDQIRYL 415
Query: 445 INCGVIPPFCNLLSCKDTQVINVVLDGLSNMLKMA----GDNAEQV---ANMIEECGGID 603
+ G I P CNLL+C+D ++I V LDG+ N+L++ +N +++ A +E+ GG+D
Sbjct: 416 VEQGAIKPLCNLLACQDNKIIQVALDGIENILRVGELDRANNPDKINLYAVYVEDAGGMD 475
Query: 604 KIEDLQTHXKMEIYKMAYDII 666
I + Q EIY+ AY+II
Sbjct: 476 LIHECQNSSNSEIYQKAYNII 496
Score = 79.4 bits (187), Expect = 4e-16
Identities = 46/176 (26%), Positives = 86/176 (48%)
Frame = +1
Query: 133 ALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWFLSNITAGNKQQVQA 312
AL A+ + G +E+ Q +++ LL HP ++ A+ + NI G+ Q Q
Sbjct: 268 ALWAISYLSDGANEKIQAIIDAGIPRRLVELLMHPSAQVQTPALRSVGNIVTGDDVQTQV 327
Query: 313 VIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSISGTRDQVAALINCGVIPPFCNLLSCK 492
+I+ G L +++ LS +KEA W +SN++ +G Q+ +I +IPP +LL+
Sbjct: 328 IINCGALSALLSLLSSPRDGVRKEACWTISNIT-AGNSSQIQYVIEANIIPPLIHLLTTA 386
Query: 493 DTQVINVVLDGLSNMLKMAGDNAEQVANMIEECGGIDKIEDLQTHXKMEIYKMAYD 660
D ++ +SN +Q+ ++E+ G I + +L +I ++A D
Sbjct: 387 DFKIQKEACWAISNATSGGARRPDQIRYLVEQ-GAIKPLCNLLACQDNKIIQVALD 441
Score = 65.7 bits (153), Expect = 6e-12
Identities = 41/144 (28%), Positives = 72/144 (50%), Gaps = 3/144 (2%)
Frame = +1
Query: 166 TDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWFLSNITAGNKQQVQAVIDAGLLPMIV 345
T+ Q V++ + F L+H + EA W L+N+ +G+ Q V++A +P+ V
Sbjct: 110 TNPPIQKVIDAGVVPRFVEFLSHENNLLKFEASWALTNVASGSSNQTHVVVEANAVPVFV 169
Query: 346 ANLSKGEFQTQKEAAWAVSNLSISG--TRDQVAALINCGVIPPFCNLL-SCKDTQVINVV 516
+ LS E +++A WA+ N++ RD V + CGV+ P N++ S + ++
Sbjct: 170 SLLSSSEQDVREQAVWALGNIAGDSPMCRDHV---LQCGVLEPLLNIIESNRRLSMLRNS 226
Query: 517 LDGLSNMLKMAGDNAEQVANMIEE 588
LSNM + G N + N I +
Sbjct: 227 TWTLSNMCR--GKNPQPDWNSISQ 248
Score = 55.2 bits (127), Expect = 8e-09
Identities = 45/189 (23%), Positives = 85/189 (44%), Gaps = 1/189 (0%)
Frame = +1
Query: 91 IPLLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALL-THPKEKICKEAVW 267
+ LLS E V+ A+ A+GNI + VL C L ++ ++ + + + + W
Sbjct: 169 VSLLSSSEQDVREQAVWALGNIAGDSPMCRDHVLQCGVLEPLLNIIESNRRLSMLRNSTW 228
Query: 268 FLSNITAGNKQQVQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSISGTRDQVAALI 447
LSN+ G Q + ++P++ + + +A WA+S LS G +++ A+I
Sbjct: 229 TLSNMCRGKNPQPDWNSISQVIPVLSKLIYTLDEDVLVDALWAISYLS-DGANEKIQAII 287
Query: 448 NCGVIPPFCNLLSCKDTQVINVVLDGLSNMLKMAGDNAEQVANMIEECGGIDKIEDLQTH 627
+ G+ LL QV L + N+ + GD+ + +I CG + + L +
Sbjct: 288 DAGIPRRLVELLMHPSAQVQTPALRSVGNI--VTGDDVQ--TQVIINCGALSALLSLLSS 343
Query: 628 XKMEIYKMA 654
+ + K A
Sbjct: 344 PRDGVRKEA 352
Score = 54.4 bits (125), Expect = 1e-08
Identities = 36/115 (31%), Positives = 60/115 (52%)
Frame = +1
Query: 241 EKICKEAVWFLSNITAGNKQQVQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLSISG 420
E + +A+W +S ++ G +++QA+IDAG+ +V L Q Q A +V N+ ++G
Sbjct: 262 EDVLVDALWAISYLSDGANEKIQAIIDAGIPRRLVELLMHPSAQVQTPALRSVGNI-VTG 320
Query: 421 TRDQVAALINCGVIPPFCNLLSCKDTQVINVVLDGLSNMLKMAGDNAEQVANMIE 585
Q +INCG + +LLS V +SN+ AG N+ Q+ +IE
Sbjct: 321 DDVQTQVIINCGALSALLSLLSSPRDGVRKEACWTISNI--TAG-NSSQIQYVIE 372
Score = 52.4 bits (120), Expect = 6e-08
Identities = 37/147 (25%), Positives = 69/147 (46%)
Frame = +1
Query: 169 DEQTQVVLNCDALSHFPALLTHPKEKICKEAVWFLSNITAGNKQQVQAVIDAGLLPMIVA 348
D++ + L AL + E + F ++ +Q VIDAG++P V
Sbjct: 69 DKENDLELELQLPDLLKALYSDDIEAQIQATAKFRKALSKETNPPIQKVIDAGVVPRFVE 128
Query: 349 NLSKGEFQTQKEAAWAVSNLSISGTRDQVAALINCGVIPPFCNLLSCKDTQVINVVLDGL 528
LS + EA+WA++N++ SG+ +Q ++ +P F +LLS + V + L
Sbjct: 129 FLSHENNLLKFEASWALTNVA-SGSSNQTHVVVEANAVPVFVSLLSSSEQDVREQAVWAL 187
Query: 529 SNMLKMAGDNAEQVANMIEECGGIDKI 609
N +AGD + + + +CG ++ +
Sbjct: 188 GN---IAGD-SPMCRDHVLQCGVLEPL 210
Score = 38.7 bits (86), Expect = 7e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 3 DTVWAISYLTXGGNEQIQMVIXSGIVTTLXSVVV 104
D +WAISYL+ G NE+IQ +I +GI L +++
Sbjct: 267 DALWAISYLSDGANEKIQAIIDAGIPRRLVELLM 300
Score = 28.7 bits (61), Expect = 0.80
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 3 DTVWAISYLTXGGNEQIQMVIXSGIVTTL 89
+ W IS +T G + QIQ VI + I+ L
Sbjct: 351 EACWTISNITAGNSSQIQYVIEANIIPPL 379
>SPAC26H5.04 |||vacuolar import and degradation protein
Vid28|Schizosaccharomyces pombe|chr 1|||Manual
Length = 729
Score = 33.9 bits (74), Expect = 0.021
Identities = 17/76 (22%), Positives = 37/76 (48%)
Frame = +1
Query: 82 QPXIPLLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEA 261
+P I LLS + +V++ A A+ N+V + L + + + ++ + K+
Sbjct: 429 EPLIKLLSDPDTRVKSTATSAICNLVLKFSPLREKFLTTNFIDTLISNISTKDSSLRKKT 488
Query: 262 VWFLSNITAGNKQQVQ 309
VW L ++ G+ + +Q
Sbjct: 489 VWVLRHVVFGDDETIQ 504
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 28.7 bits (61), Expect = 0.80
Identities = 18/52 (34%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
Frame = -1
Query: 303 LLFI-TCRDV*QKPYRLFAYLLFRMSQ*SREV*ESI-AVQDNLSLFVCSCDN 154
LLF+ +C DV KPY +F Y F + + R E+ +V + + +F C +N
Sbjct: 1061 LLFLKSCSDVDFKPYNVFIYTAFVVPRLDRFADENTQSVSNLMKIFRCWFEN 1112
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1095
Score = 27.9 bits (59), Expect = 1.4
Identities = 22/107 (20%), Positives = 44/107 (41%)
Frame = +1
Query: 91 IPLLSHKEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVWF 270
+PLL+ +V+ AA AVG + T QV L +L P+ ++ A
Sbjct: 418 LPLLADPHPRVRWAACNAVGQMSTDFAPDMQVKYPSRILEALVPVLESPESRVQAHAAAA 477
Query: 271 LSNITAGNKQQVQAVIDAGLLPMIVANLSKGEFQTQKEAAWAVSNLS 411
+ N + +V +L ++ L + Q++A ++ ++
Sbjct: 478 MVNFSEEADNKVLEPYLDDILQRLLTLLQSPKRYVQEQAVTTIATVA 524
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 27.1 bits (57), Expect = 2.4
Identities = 13/37 (35%), Positives = 20/37 (54%)
Frame = +1
Query: 157 VTGTDEQTQVVLNCDALSHFPALLTHPKEKICKEAVW 267
V G +++TQ CD + H A+ + IC E+VW
Sbjct: 687 VVGNEDRTQ----CDHVFHVNAIFKPSRCYICSESVW 719
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 25.4 bits (53), Expect = 7.4
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +1
Query: 430 QVAALINCGVIPPFCNLLSC 489
Q+A L+ C ++P NL+ C
Sbjct: 554 QIALLLGCSILPHLKNLVDC 573
>SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1253
Score = 25.0 bits (52), Expect = 9.8
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = +1
Query: 412 ISGTRDQVAALINCGVIPPFCNLLSCKDTQVINVVLDGLSNMLKMAGD 555
I G+ V L C + PP C D V ++ S+ML A D
Sbjct: 392 IDGSSLLVTPLSLCNIPPPMCRYKLSLDYNVQMTSINATSDMLFAADD 439
>SPBC1604.12 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 860
Score = 25.0 bits (52), Expect = 9.8
Identities = 10/38 (26%), Positives = 21/38 (55%)
Frame = +1
Query: 529 SNMLKMAGDNAEQVANMIEECGGIDKIEDLQTHXKMEI 642
+N+ + +NA+ +++ + G ID L TH K+ +
Sbjct: 101 ANITTNSNNNAQTISSSSQSVGHIDNPSGLGTHGKVRL 138
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,751,615
Number of Sequences: 5004
Number of extensions: 55291
Number of successful extensions: 165
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -