Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P14_F_J16
         (602 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc...    52   6e-08
SPBC13G1.07 |||palmitoyltransferase|Schizosaccharomyces pombe|ch...    31   0.098
SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|ch...    27   1.6  
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...    27   2.1  
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po...    27   2.8  
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi...    26   4.9  
SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr 2|||...    26   4.9  
SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase Wis1|Schizosaccha...    25   8.5  
SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces ...    25   8.5  
SPCC1529.01 ||SPCC794.14|membrane transporter|Schizosaccharomyce...    25   8.5  

>SPAC1A6.09c |lag1||sphingosine N-acyltransferase
           Lag1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 390

 Score = 52.0 bits (119), Expect = 6e-08
 Identities = 41/178 (23%), Positives = 74/178 (41%), Gaps = 1/178 (0%)
 Frame = +3

Query: 30  FVIQNHADIVSCVVMVFLVGLMVQSTSPIASLFISLHHNVSGVEPTREAPKGEPFTYESG 209
           F++    +I   ++ +    L+  S  P A  FI L +         + P G   +Y  G
Sbjct: 64  FLLVWQKEICLSIIAICFACLLSPSLRPYAEPFIFLSY---------KQPDG---SYGKG 111

Query: 210 WKDACAVFFYSLVCIVMHAILQEYFLDKISKKFHLSKSR-LSALNESGQLIVFHLVTLVW 386
            KDAC   F+ +V      I+ +Y        + +   + +    E G    ++L     
Sbjct: 112 PKDACFPIFWVIVFTAFRVIVMDYVFRPFVLNWGVRNRKVIIRFCEQGYSFFYYLCFWFL 171

Query: 387 GGDAILREGFIFNISQLWEGYPVHPMSFLLKLWWVVQASYWLHTIPELYFQRIKKDEW 560
           G        +  N  +L+E YP + MS L K ++++Q  +WL  I  L+ ++ + D W
Sbjct: 172 GLYIYRSSNYWSNEEKLFEDYPQYYMSPLFKAYYLIQLGFWLQQILVLHLEQRRADHW 229


>SPBC13G1.07 |||palmitoyltransferase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 356

 Score = 31.5 bits (68), Expect = 0.098
 Identities = 30/141 (21%), Positives = 58/141 (41%), Gaps = 2/141 (1%)
 Frame = +3

Query: 84  VGLMVQSTSPIASLFISLHHNVSGVEPTR--EAPKGEPFTYESGWKDACAVFFYSLVCIV 257
           + ++     P  SL+I+   N   ++     EA +  P+ Y+  + + C+   +      
Sbjct: 112 ISVLTSVLLPYISLYIAAKSNPGKIDLKNWNEASRRFPYDYKIFFPNKCSTCKFEKPARS 171

Query: 258 MHAILQEYFLDKISKKFHLSKSRLSALNESGQLIVFHLVTLVWGGDAILREGFIFNISQL 437
            H  L    ++K      +  +    LN +    +F L T+     +ILR G+ FN  + 
Sbjct: 172 KHCRLCNICVEKFDHHC-IWINNCVGLNNARYFFLFLLCTIQLLFHSILRLGYHFNALRD 230

Query: 438 WEGYPVHPMSFLLKLWWVVQA 500
              YP    SFL   W+ +++
Sbjct: 231 MRQYP----SFLRSWWFAIKS 247


>SPAC2F7.10 |||palmitoyltransferase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 642

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 15/68 (22%), Positives = 28/68 (41%), Gaps = 1/68 (1%)
 Frame = +2

Query: 53  YCFMCCYGFPGWIDGTVYKSHCK-LVHQFTPQCQWSGTNERSAERRTFYL*IWVERCLCR 229
           YC  C    P     + +   CK  ++++   C W+G    +   RTF L ++    L  
Sbjct: 401 YCLKCFQVKP---PRSYHCGACKRCINRYDHHCPWTGNCVGARNHRTFLLFVFTLSTLIP 457

Query: 230 VFLFTCLY 253
           ++ +   Y
Sbjct: 458 IYFYVAFY 465


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 24/92 (26%), Positives = 37/92 (40%)
 Frame = +3

Query: 177 PKGEPFTYESGWKDACAVFFYSLVCIVMHAILQEYFLDKISKKFHLSKSRLSALNESGQL 356
           P G   T  S W+          VC VM+  +Q+YF + +      +++ L  ++E    
Sbjct: 646 PPGLDATAYSSWEKGDNFVTKKNVCTVMNLWVQKYFFEDLK-----ARNTLYLISEMRTF 700

Query: 357 IVFHLVTLVWGGDAILREGFIFNISQLWEGYP 452
           +  H+V     G  IL E     I  LW   P
Sbjct: 701 LRDHVVPSFHIGSVILSE-----IDNLWTEEP 727


>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1313

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 14/44 (31%), Positives = 25/44 (56%)
 Frame = +3

Query: 318 KSRLSALNESGQLIVFHLVTLVWGGDAILREGFIFNISQLWEGY 449
           + +L+ LN     ++ H ++ +   D++LR+     ISQLWE Y
Sbjct: 612 QGQLACLNPQ---VLSHCLSHLNSPDSLLRQWACLCISQLWENY 652


>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 22/76 (28%), Positives = 31/76 (40%), Gaps = 1/76 (1%)
 Frame = +3

Query: 126 FISLHHNVSGVEPTREAPKGEPFTYES-GWKDACAVFFYSLVCIVMHAILQEYFLDKISK 302
           FI+    +S + P  E PK      E  GW   C V +  L  I ++   +   LD I+ 
Sbjct: 6   FINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDPINN 65

Query: 303 KFHLSKSRLSALNESG 350
              L  S  S L  +G
Sbjct: 66  FRVLDGSSASGLMITG 81


>SPBC4.06 |||acid phosphatase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 462

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 11/37 (29%), Positives = 16/37 (43%)
 Frame = +3

Query: 108 SPIASLFISLHHNVSGVEPTREAPKGEPFTYESGWKD 218
           +P+     SL H  SG+ P  +   G P  +   W D
Sbjct: 149 TPMVRALESLEHVFSGLYPESKRKMGLPVIFTRNWSD 185


>SPBC409.07c |wis1|spc2, smf2|MAP kinase kinase
           Wis1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 605

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 13/45 (28%), Positives = 20/45 (44%)
 Frame = +2

Query: 407 GRFHFQHLSALGRLPSTPNELPSQVMVGCPSFLLVAHNPRIVLPT 541
           G +   +    GRL ++P + P+    G    L  A NP +  PT
Sbjct: 181 GSYPLPNSQLAGRLSNSPVKSPNMPESGLAKSLAAARNPLLNRPT 225


>SPAC10F6.13c |||aspartate aminotransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 409

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 12/26 (46%), Positives = 19/26 (73%), Gaps = 3/26 (11%)
 Frame = +3

Query: 105 TSPIASLFISLH---HNVSGVEPTRE 173
           ++P  S+F+ LH   HN +G++PTRE
Sbjct: 176 SAPEGSIFL-LHACAHNPTGIDPTRE 200


>SPCC1529.01 ||SPCC794.14|membrane transporter|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 462

 Score = 25.0 bits (52), Expect = 8.5
 Identities = 12/33 (36%), Positives = 20/33 (60%)
 Frame = +3

Query: 285 LDKISKKFHLSKSRLSALNESGQLIVFHLVTLV 383
           LD+I+ +FH+  S   AL  S  L+VF +  ++
Sbjct: 69  LDQIADRFHIQNSTEKALILSIYLLVFAISPMI 101


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,872,101
Number of Sequences: 5004
Number of extensions: 64365
Number of successful extensions: 176
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)