Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_J12
(711 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc... 169 3e-43
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 39 8e-04
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 33 0.030
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 33 0.040
SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces pomb... 28 1.1
SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc... 26 4.6
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 6.1
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 25 8.1
SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc... 25 8.1
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 25 8.1
>SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 240
Score = 169 bits (411), Expect = 3e-43
Identities = 78/176 (44%), Positives = 123/176 (69%)
Frame = +2
Query: 95 LVLIAATVCTXSGKALVSRQFVEMTKARIEGLLAAFPKLMTGGRQHTFVETESVRYVYQP 274
+V++A ++ GKA++SRQF EM++ R+E LL++FP L++ Q+T VE+++VR+VYQP
Sbjct: 1 MVVLAVSIVNRGGKAIISRQFREMSRVRVESLLSSFPALVSEKSQNTTVESDNVRFVYQP 60
Query: 275 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALG 454
LD+LY++LIT SNIL+D++TL L S+VV C L E E+L AF + AFDE +LG
Sbjct: 61 LDELYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLG 120
Query: 455 YRESVNLAQVRSFVEMDSHEEKIYQAVRQTQXXXXXXXXXXXXXXLQRERLEAAKR 622
YR++V+L Q+++++EM+SHEEKI + V + + L+ ++ EAA+R
Sbjct: 121 YRDNVSLTQIKTYLEMESHEEKIQEIVSRNKEIEATEERKRRIKQLELQKKEAARR 176
>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 38.7 bits (86), Expect = 8e-04
Identities = 29/140 (20%), Positives = 63/140 (45%), Gaps = 2/140 (1%)
Frame = +2
Query: 107 AATVCTXSGKALVSRQF-VEMTKARIEGLLAAFPKLMTG-GRQHTFVETESVRYVYQPLD 280
A V GK ++SR + ++ + +E L ++ G + E + Y+Y +
Sbjct: 4 AIFVLNLKGKVIISRDYRADIPMSVVEKFLPLKSEVEEEQGFSTPCLTHEGINYIYIHHN 63
Query: 281 KLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAFNLLFAFDEIVALGYR 460
+Y+L ++ S+ +E L LR + V +Y +L E + + + DEI+ G+
Sbjct: 64 DVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEIMDFGFP 123
Query: 461 ESVNLAQVRSFVEMDSHEEK 520
++ ++ ++ S+ K
Sbjct: 124 QTTETKILQEYITQTSNTVK 143
>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 446
Score = 33.5 bits (73), Expect = 0.030
Identities = 20/76 (26%), Positives = 34/76 (44%)
Frame = +2
Query: 227 QHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLN 406
+H V S Y+Y + LY++ IT N++ LE L + + Y +L E V +
Sbjct: 43 RHPIVSIGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKD 102
Query: 407 QAFNLLFAFDEIVALG 454
+ DE++ G
Sbjct: 103 NVSFIFELLDEMIDYG 118
>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 143
Score = 33.1 bits (72), Expect = 0.040
Identities = 21/91 (23%), Positives = 37/91 (40%)
Frame = +2
Query: 227 QHTFVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLN 406
Q F+E E+ + VY+ LY N L LE + F ++ + + E +++
Sbjct: 47 QANFLEWENSKLVYRRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIF 106
Query: 407 QAFNLLFAFDEIVALGYRESVNLAQVRSFVE 499
+ + DEI+ G N V +E
Sbjct: 107 NFYKVSAILDEIILGGEIGESNKKSVLERIE 137
>SPAC1B1.01 |||transcription factor Rdp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 478
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +3
Query: 591 CSVRDWRRPNVASRRAHRCPSAVALVARRCPRRLH 695
C ++WRRP + A +CPS +A+ P ++
Sbjct: 189 CGRQNWRRPVNFASHAKQCPSFLAMDPNNIPPEIN 223
>SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 162
Score = 26.2 bits (55), Expect = 4.6
Identities = 18/87 (20%), Positives = 37/87 (42%)
Frame = +2
Query: 236 FVETESVRYVYQPLDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTETEVLNQAF 415
FVE + + VY+ L+ + + N L LE + F + +Y + E +++
Sbjct: 50 FVEYKGEKIVYRRYASLFFVCGIEQDDNELIILEVIHKFVECLDKYFGNVCELDLIFNFE 109
Query: 416 NLLFAFDEIVALGYRESVNLAQVRSFV 496
+ +E++ G + + V S V
Sbjct: 110 KAYYVMEELLLAGELQESSKTNVLSAV 136
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1
Query: 384 SCTQYSGTTLLKSLNVSRSSRMLLAFVVISNIYN 283
SC + L S+N + +SR LLA +V +I N
Sbjct: 134 SCDSNENLSTLSSVNQNFNSRQLLATIVPESIQN 167
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 25.4 bits (53), Expect = 8.1
Identities = 8/28 (28%), Positives = 15/28 (53%)
Frame = -1
Query: 234 VCCRPPVISLGKAASRPSMRAFVISTNW 151
+CC P +++ + +PS R+F W
Sbjct: 2128 LCCDPSRLNIEQELQKPSGRSFACKKRW 2155
>SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase
Ubp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1141
Score = 25.4 bits (53), Expect = 8.1
Identities = 14/36 (38%), Positives = 26/36 (72%), Gaps = 1/36 (2%)
Frame = +2
Query: 218 GGRQHTFVE-TESVRYVYQPLDKLYMLLITTKASNI 322
GGR T +E ++++ Y+ L KL++ LIT+K+S++
Sbjct: 664 GGRIVTRIEFLRALQFTYE-LRKLFIELITSKSSSV 698
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 25.4 bits (53), Expect = 8.1
Identities = 26/92 (28%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
Frame = +2
Query: 275 LDKLYMLLITTKASNILEDLETLRLFSRVVPEYCVQLTET-EVLNQAFNLLFAFDEIVAL 451
L L MLL + I+E L + L S +PE C T E++N N IVA
Sbjct: 1197 LQCLKMLLEVSDRKLIVEKLRPILLPSFEIPEPCNTATSVQELINPEINQ--DDSPIVAS 1254
Query: 452 GYRESVNLAQVRSFVEMDSHEEKIYQAVRQTQ 547
Y + L + ++ + +E + A++Q Q
Sbjct: 1255 RYCTTSFLHRFYEYLLSGNKDEALTYALQQKQ 1286
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,684,315
Number of Sequences: 5004
Number of extensions: 51399
Number of successful extensions: 128
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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