Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_J02
(769 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 30 0.42
SPAC1782.05 |||phosphotyrosyl phosphatase activator homolog|Schi... 29 0.55
SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase Ppk38|Schizo... 29 0.73
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 28 1.7
SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 27 3.0
SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub... 27 3.0
SPCC1682.09c |||guanine nucleotide transporter|Schizosaccharomyc... 27 3.9
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 27 3.9
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 26 5.2
SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |... 26 5.2
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 26 6.8
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 25 9.0
SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr 2... 25 9.0
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 29.9 bits (64), Expect = 0.42
Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +2
Query: 242 VQSIRNFLSEPLSNVETSFSPQIYQYSSPVNEHV----TNIVNRQPEISNPQPSTSGMTS 409
V + ++++ S+ S P+ + SS ++E + TN + +PE S +TSG+TS
Sbjct: 936 VSTASSYIASSASSNTLSTEPKTFSSSSTLSESISSINTNSLTVKPESSLSSSTTSGLTS 995
Query: 410 RYNT 421
+T
Sbjct: 996 SSST 999
>SPAC1782.05 |||phosphotyrosyl phosphatase activator
homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 352
Score = 29.5 bits (63), Expect = 0.55
Identities = 27/83 (32%), Positives = 37/83 (44%), Gaps = 1/83 (1%)
Frame = +2
Query: 194 DGQDFSDAESDDDIEKVQSIRNFLSEPLSNVETSFSPQIYQYSSPVNEHVTNIVNRQPEI 373
D +D + D + + S L E + + S S I Q SS EHV NI++R EI
Sbjct: 19 DNEDLKLFKEGDAYKLIDSFICDLDEAVKDKPISAS--IPQSSSI--EHVLNILDRVGEI 74
Query: 374 SNPQPSTSGMTSRY-NTRFNYKY 439
P+ M SR+ N F Y
Sbjct: 75 KQENPAIDNMGSRFGNPAFQSFY 97
>SPCP1E11.02 |ppk38||Ark1/Prk1 family protein kinase
Ppk38|Schizosaccharomyces pombe|chr 3|||Manual
Length = 650
Score = 29.1 bits (62), Expect = 0.73
Identities = 18/69 (26%), Positives = 30/69 (43%)
Frame = +2
Query: 236 EKVQSIRNFLSEPLSNVETSFSPQIYQYSSPVNEHVTNIVNRQPEISNPQPSTSGMTSRY 415
E +Q + + ++S PQ P+ + VN P +SN + +G TS
Sbjct: 331 EYLQHASKLENSGIHQSKSSVFPQPASAMKPMASPMLPNVNSMPYLSNGDHNNNGNTSSP 390
Query: 416 NTRFNYKYH 442
+RF+Y H
Sbjct: 391 VSRFSYGQH 399
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 27.9 bits (59), Expect = 1.7
Identities = 12/23 (52%), Positives = 17/23 (73%)
Frame = +3
Query: 552 WKKTYFQ*RPSSFTGNANFHSPI 620
WK F+ +PSSF GNA+F++ I
Sbjct: 195 WKNVIFKIKPSSF-GNASFYNAI 216
>SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1234
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = +1
Query: 556 RKHIFNNALLHLLETQISIHRL*TLKLPFFI 648
R HI NAL+ +L + ++H+L LP+ +
Sbjct: 180 RSHILFNALISILSSSSTLHKLYPSGLPYSV 210
>SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory
subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 838
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +2
Query: 200 QDFSDAESDDDIEKVQSIRNFLSEPLSNVETS 295
+ F D ++++ + +QSI NF+ + L + ETS
Sbjct: 142 EHFLDKKTEETVAFIQSIDNFVEKILRHAETS 173
Score = 25.4 bits (53), Expect = 9.0
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +2
Query: 194 DGQDFSDAESDDDIEKVQSIRNFLSEPLSN 283
D +DFSD+E +D + R F E S+
Sbjct: 801 DREDFSDSEEEDGNDSSDDDRGFAEEEYSD 830
>SPCC1682.09c |||guanine nucleotide transporter|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 300
Score = 26.6 bits (56), Expect = 3.9
Identities = 13/49 (26%), Positives = 27/49 (55%)
Frame = -1
Query: 193 FTPRRNIPETFNIFITQSSSRHYLFKIYTSLQHRIFLESKLFNNYFLNI 47
+T RN P +F +F + ++ Y+FK+ + + ++ F N+F +I
Sbjct: 177 WTAARNAPGSFALFGGNAFAKEYIFKL------KDYSQATFFQNFFTSI 219
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 26.6 bits (56), Expect = 3.9
Identities = 19/67 (28%), Positives = 35/67 (52%), Gaps = 4/67 (5%)
Frame = +2
Query: 272 PLSNVETSFSPQIYQYSSP-VNEHVTNIVNRQP---EISNPQPSTSGMTSRYNTRFNYKY 439
P+S+ +++S + SS V+ + IV+ P S+P PS+S + S Y+ +
Sbjct: 658 PISSSLSTYSSSVIPSSSTLVSSSSSLIVSSSPVASSSSSPIPSSSSLVSTYSASLSNIT 717
Query: 440 HCTISGT 460
H ++S T
Sbjct: 718 HSSLSLT 724
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 26.2 bits (55), Expect = 5.2
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = -3
Query: 227 HHFQHQKNLDHLHSQKE 177
HH +H N DH+H E
Sbjct: 540 HHHEHNHNHDHIHKYNE 556
>SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1032
Score = 26.2 bits (55), Expect = 5.2
Identities = 13/52 (25%), Positives = 28/52 (53%)
Frame = -1
Query: 223 TFSIRKILTIFTPRRNIPETFNIFITQSSSRHYLFKIYTSLQHRIFLESKLF 68
TF ++T+F ++N + F I +SS + ++ S+++ +LE + F
Sbjct: 242 TFLSLPVITVFNGKKNRKQRFRIRSWRSSLNFLVKELDKSIKNFAYLEHRTF 293
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 25.8 bits (54), Expect = 6.8
Identities = 10/39 (25%), Positives = 21/39 (53%)
Frame = +2
Query: 242 VQSIRNFLSEPLSNVETSFSPQIYQYSSPVNEHVTNIVN 358
+QS+ N S ++++ SF P + +Y V+ + + N
Sbjct: 374 IQSVANGTSSTKTHLDVSFQPMLKEYEEKVHNLLRKLGN 412
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 25.4 bits (53), Expect = 9.0
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +2
Query: 395 SGMTSRYNTRFNYKYHCTISGT*TI 469
S + +Y+TRF+YK C+ S + T+
Sbjct: 130 SSLQEKYSTRFSYKAGCSDSCSVTV 154
>SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 479
Score = 25.4 bits (53), Expect = 9.0
Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = +2
Query: 272 PLSNVETSFSPQ---IYQYSSPVNEHVTNIVNRQPEISNPQPSTSGMTSR 412
PLS+ ET + ++ + +N++ + I N P+I +PS S SR
Sbjct: 217 PLSDYETDDMDDGTPLQRHVATLNQYQSPIFNLGPDILEDEPSYSSRASR 266
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,210,804
Number of Sequences: 5004
Number of extensions: 70294
Number of successful extensions: 224
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 204
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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