Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_E06
(696 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 291 8e-80
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 154 1e-38
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 154 1e-38
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 138 5e-34
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 31 0.16
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 27 1.9
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 4.5
SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 25 7.8
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.8
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 291 bits (713), Expect = 8e-80
Identities = 130/192 (67%), Positives = 153/192 (79%)
Frame = +1
Query: 118 LGAKFWXLISDXHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDS 297
+GA FW I+D HG+D G YHG S+ Q ER+NVY+NEA+GGKYVPRA+LVDLEPGTMD+
Sbjct: 16 VGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDA 75
Query: 298 VRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQX 477
V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+CD LQGFQ
Sbjct: 76 VKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQL 135
Query: 478 XXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLXEN 657
KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQL EN
Sbjct: 136 THSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVEN 195
Query: 658 TDETYCIDNEAL 693
+DET+CIDNEAL
Sbjct: 196 SDETFCIDNEAL 207
Score = 31.5 bits (68), Expect = 0.12
Identities = 12/15 (80%), Positives = 14/15 (93%)
Frame = +3
Query: 72 MRAIVHLQAGQCGHQ 116
MR IVH+QAGQCG+Q
Sbjct: 1 MREIVHIQAGQCGNQ 15
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 154 bits (373), Expect = 1e-38
Identities = 74/199 (37%), Positives = 112/199 (56%), Gaps = 6/199 (3%)
Frame = +1
Query: 118 LGAKFWXLISDXHGIDPTG------AYHGDSDLQLERINVYYNEASGGKYVPRAILVDLE 279
+G W L HGI P G H ++ + +++E GK+VPR+I VDLE
Sbjct: 16 IGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLE 75
Query: 280 PGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 459
P +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C
Sbjct: 76 PNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSG 135
Query: 460 LQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSV 639
LQGF ++ EY + +SV P+P+VS +VVEPYN+ L+
Sbjct: 136 LQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTT 195
Query: 640 HQLXENTDETYCIDNEALY 696
H +N+D T+ +DNEA Y
Sbjct: 196 HATLDNSDCTFMVDNEACY 214
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 154 bits (373), Expect = 1e-38
Identities = 74/197 (37%), Positives = 110/197 (55%), Gaps = 2/197 (1%)
Frame = +1
Query: 112 TSLGAKFWXLISDXHGIDPTGAYHGDSDLQLER--INVYYNEASGGKYVPRAILVDLEPG 285
T +G W L HGI P G + ++ Q + +++E GKYVPR+I VDLEP
Sbjct: 14 TQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPN 73
Query: 286 TMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQ 465
+D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +R+ +++C LQ
Sbjct: 74 VIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQ 133
Query: 466 GFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQ 645
GF ++ EY + +SV P+P+VS +VVEPYN+ L+ H
Sbjct: 134 GFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHA 193
Query: 646 LXENTDETYCIDNEALY 696
+ D T+ +DNE+ Y
Sbjct: 194 TLDLADCTFMVDNESCY 210
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 138 bits (335), Expect = 5e-34
Identities = 64/195 (32%), Positives = 112/195 (57%), Gaps = 3/195 (1%)
Frame = +1
Query: 118 LGAKFWXLISDXHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDS 297
+G++FW + HGI P G + ++R +V++ ++ +Y+PRAIL+DLEP +++
Sbjct: 17 IGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNN 76
Query: 298 VRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGF 471
+ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D++ +E++ D L+GF
Sbjct: 77 ILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGF 135
Query: 472 QXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQL 648
++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L
Sbjct: 136 SLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRL 195
Query: 649 XENTDETYCIDNEAL 693
N D +DN AL
Sbjct: 196 TLNADSVVVLDNAAL 210
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/14 (71%), Positives = 12/14 (85%)
Frame = +3
Query: 75 RAIVHLQAGQCGHQ 116
R I+ LQAGQCG+Q
Sbjct: 3 REIITLQAGQCGNQ 16
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 31.1 bits (67), Expect = 0.16
Identities = 29/105 (27%), Positives = 50/105 (47%)
Frame = +2
Query: 377 PRDTTQRVLSSLTRSSM*SAKNQNLAIAYRASNLHIPSVAAPGPVWAPSSSQRSVKSTPT 556
PR++T++ L S++ S S+ Q + +S L+ PS AP P + SSS T
Sbjct: 219 PRESTEKPLLSVSALSS-SSHLQPTSATSSSSRLY-PSRPAPTPPASSSSSPLLSSQTVK 276
Query: 557 ES*THTQ*SPRPKYQTLSSNHTMRLSQFTSXLKTQTKPTASTTRL 691
+ ++ P P + S+++ +R S L QT T+ T +
Sbjct: 277 TTTSNASRQPSPLVSSKSTDNIIR-SHSPVLLTPQTLSTSETKHI 320
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +1
Query: 376 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 465
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 27.5 bits (58), Expect = 1.9
Identities = 15/35 (42%), Positives = 18/35 (51%)
Frame = +2
Query: 200 SWSASMYTTMKPPAASTCPAPFSSTWSPAPWTLSA 304
S +AS PP A+ + SST SPAPW A
Sbjct: 396 SGAASPQAGHPPPWAAASTSVSSSTSSPAPWAKPA 430
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 307 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 408
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 258
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +2
Query: 449 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 544
LA+ Y + + +V APG +W+P+ +R K
Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189
>SPAC8C9.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 647
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +2
Query: 206 SASMYTTMKPPAASTCPAPFSSTWSPAP 289
S S T P +A+T P P S+T P P
Sbjct: 560 SGSATTIPSPGSATTKPTPGSATTKPTP 587
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 328 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 459
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.8
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 365 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 270
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.136 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,676,659
Number of Sequences: 5004
Number of extensions: 53778
Number of successful extensions: 193
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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