BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P14_F_A11
(829 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC227.05 |||prefoldin subunit 4|Schizosaccharomyces pombe|chr ... 51 2e-07
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 34 0.028
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 32 0.11
SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pomb... 31 0.20
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 29 0.80
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 29 0.80
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 29 1.1
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 28 1.4
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 27 2.5
SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces pomb... 27 2.5
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 27 2.5
SPAC7D4.04 |taf1||Taz1 interacting factor 1|Schizosaccharomyces ... 27 3.2
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 27 3.2
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 27 3.2
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 26 5.7
SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting... 26 5.7
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 26 5.7
SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces p... 25 9.9
>SPAC227.05 |||prefoldin subunit 4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 123
Score = 50.8 bits (116), Expect = 2e-07
Identities = 30/113 (26%), Positives = 63/113 (55%), Gaps = 3/113 (2%)
Frame = +2
Query: 122 VHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSL--ADDSEKIPYL- 292
V + EDQ+ +N+F+ L+++ + ++K + ++++ +A E L DD + IP L
Sbjct: 4 VPVLAEDQRNLNEFSVLHSRKAIQELDIKNLKTQIEDIVDAKNECELLDEDDGDDIPALK 63
Query: 293 IGEIFICQNLEITLKNLEEAKAKKIHXIHDLEEKCEELKSQMSDLKAHLYGKF 451
+G+ F +L + L LE+++ + L E+ ++++ +LK+ LY KF
Sbjct: 64 VGDAFFQVSLPVLLDQLEQSEESLEKQVDVLRSSMEKDETRIQELKSMLYSKF 116
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 33.9 bits (74), Expect = 0.028
Identities = 26/102 (25%), Positives = 50/102 (49%), Gaps = 3/102 (2%)
Frame = +2
Query: 143 QQKINKFARLN-AKVDDYKDELKVKQNDVKNLEEAVEELSLADDS--EKIPYLIGEIFIC 313
Q +NK+ R++ ++++ K + + + + LE ++E + D+ +++ L E+
Sbjct: 1340 QSVLNKYERVDPTQLEELKKNCEALEKEKQELETKLQETAKETDTFKQQVNSLNEEV--- 1396
Query: 314 QNLEITLKNLEEAKAKKIHXIHDLEEKCEELKSQMSDLKAHL 439
+NL+ K +E+A K EKCE LK AHL
Sbjct: 1397 ENLK---KEVEQANTKNTRLAAAWNEKCENLKKSSLTRFAHL 1435
Score = 26.2 bits (55), Expect = 5.7
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +2
Query: 167 RLNAKVDDYKDELKVKQNDVKNLEEAVEELS 259
R AK+ Y+ + + QN + LEE +E L+
Sbjct: 1673 RSKAKISVYEKKTRDLQNKITQLEETIENLN 1703
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 31.9 bits (69), Expect = 0.11
Identities = 23/79 (29%), Positives = 45/79 (56%), Gaps = 3/79 (3%)
Frame = +2
Query: 212 KQNDVKNLEEAVEELS--LADDSEKIPYLIGEIFICQNLEITLKNLEEAKAKKIHXIHDL 385
K+N+ KN+ + +E LS L + +++ ++ G+ +I L+ L AK ++H I+ L
Sbjct: 106 KKNEAKNMYKEIERLSVILGESVDEVNFVNGDFEDIS--DIKLEQL-SAKLNELHQIYRL 162
Query: 386 -EEKCEELKSQMSDLKAHL 439
+EK E+K ++ L+ L
Sbjct: 163 RKEKNIEIKEELKKLQDSL 181
>SPCC285.16c |msh6||MutS protein homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1254
Score = 31.1 bits (67), Expect = 0.20
Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +2
Query: 95 KGTFQPDSDVHISYEDQQKINKFAR--LNAKVDDYKDELKVKQNDVKNLEEAVEELSLAD 268
+G F+P+ Y+ QK + L A ++ YK +L+ + KN+ + V ++ +
Sbjct: 827 EGVFEPEIGFEAEYDTSQKYQSELKNELYALLEQYKKQLRCSSLNFKNIGKEVYQVEVPS 886
Query: 269 DSEKIP 286
D K+P
Sbjct: 887 D-VKVP 891
>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1208
Score = 29.1 bits (62), Expect = 0.80
Identities = 13/47 (27%), Positives = 27/47 (57%)
Frame = +2
Query: 113 DSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEE 253
+ +V YE +Q N+ L+ ++D +D L K++++ L+ +EE
Sbjct: 278 NKEVDYEYEIRQLQNRLDELSEELDVAQDLLTEKEDEIATLKRQIEE 324
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 29.1 bits (62), Expect = 0.80
Identities = 23/96 (23%), Positives = 45/96 (46%)
Frame = +2
Query: 152 INKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQNLEIT 331
++ F ++N K + + L + + +E+L D +LI + + L+ +
Sbjct: 1294 LDAFNQVNFKKMELDNRLTTTDAEFTKVVADLEKLQHEHDD----WLIQRGDLEKALKDS 1349
Query: 332 LKNLEEAKAKKIHXIHDLEEKCEELKSQMSDLKAHL 439
KN +A+ IH LEE EE K ++++L + L
Sbjct: 1350 EKNFLRKEAEMTENIHSLEEGKEETKKEIAELSSRL 1385
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.7 bits (61), Expect = 1.1
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +2
Query: 314 QNLEITLKNLEEAKAKKIHXIHDLEEKCEELK 409
QNL TLKN+E+A+ + I D+++ E++
Sbjct: 117 QNLSATLKNIEQARPTEEITIEDMKQAVPEIE 148
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 28.3 bits (60), Expect = 1.4
Identities = 33/125 (26%), Positives = 57/125 (45%), Gaps = 4/125 (3%)
Frame = +2
Query: 68 LIVNMSTTAKGTFQPDSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAV 247
++ N T K +F S+V +SYE + N L+ V+D + +L+ K+ +L
Sbjct: 540 ILANQLMTLKSSF---SEV-MSYELKDDDNYNEELDKLVEDVRKKLQEKEEAESSLRSVR 595
Query: 248 EELSLADDSEKIPYLIGEI-FICQNLEI---TLKNLEEAKAKKIHXIHDLEEKCEELKSQ 415
E L + +I + I + +N +I TLK+ A I I LE + EE +
Sbjct: 596 ERLEI-----RISLSVQSINDLTENKKIKTKTLKSYSGTFASMISEIKALESEIEENRKT 650
Query: 416 MSDLK 430
+ L+
Sbjct: 651 LHSLQ 655
>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
Sen1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1687
Score = 27.5 bits (58), Expect = 2.5
Identities = 27/94 (28%), Positives = 47/94 (50%), Gaps = 4/94 (4%)
Frame = +2
Query: 146 QKINKFAR-LNAKVDDYKDELKVKQN--DVKNLEEAVE-ELSLADDSEKIPYLIGEIFIC 313
+ IN R L+ + K L+V Q D+ +L+E + D +KI L +I +
Sbjct: 1234 ETINVSVRDLSLEYQTEKQLLEVNQGAIDLGSLQELTRWRDTFYDCIQKIEELEKQIDVA 1293
Query: 314 QNLEITLKNLEEAKAKKIHXIHDLEEKCEELKSQ 415
+++ K+L + KI+ + E+K EEL+SQ
Sbjct: 1294 RDVAEDTKSLGKELQNKINEKNLAEQKVEELQSQ 1327
>SPBC365.05c |slu7||splicing factor Slu7|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 27.5 bits (58), Expect = 2.5
Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +2
Query: 182 VDDYKDEL-KVKQNDVKNLEEAVEELSLADDSEK 280
+D Y D KVK KN E+ V +L++ D SEK
Sbjct: 325 LDRYGDGTSKVKDKKAKNNEKEVPDLAILDKSEK 358
>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein
3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1385
Score = 27.5 bits (58), Expect = 2.5
Identities = 18/73 (24%), Positives = 38/73 (52%), Gaps = 5/73 (6%)
Frame = +2
Query: 107 QPDSDVHISYEDQQKINKFARLNAKVDDYKDELKV-----KQNDVKNLEEAVEELSLADD 271
+PDS+ + ++ ++ + RL +VD++ E+K K N + EEA ++ D
Sbjct: 1166 KPDSEARLQAIERSELLQKKRLLQRVDEFSKEVKTFVENEKLNKIGGAEEADRIRTIQD- 1224
Query: 272 SEKIPYLIGEIFI 310
EK+ ++ + F+
Sbjct: 1225 -EKVRKILWDDFV 1236
>SPAC7D4.04 |taf1||Taz1 interacting factor 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 926
Score = 27.1 bits (57), Expect = 3.2
Identities = 19/95 (20%), Positives = 45/95 (47%), Gaps = 4/95 (4%)
Frame = +2
Query: 152 INKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQNLEIT 331
+N ++ KVD ++ + + +L++ E +LA D+E + ++I E +
Sbjct: 641 LNLYSVFEGKVDSLQELYNAFKLQLTSLKQKGEYATLAKDAEAVQHIIEERDYALAEKAD 700
Query: 332 LKNLEEAKAKK----IHXIHDLEEKCEELKSQMSD 424
L L E + ++ ++ + +C EL+S + +
Sbjct: 701 LLKLSENRKEQCKILTQKLYTIVFRCNELQSVLRE 735
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.1 bits (57), Expect = 3.2
Identities = 16/61 (26%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +2
Query: 215 QNDVKNLEEAVEELSLADDSEKIPYLIGEIFICQ-NLEITLKNLEEAKAKKIHXIHDLEE 391
Q+ + +LE+ V++ + ++ P + + + LE K +EEAKAK+ + DL +
Sbjct: 4 QDSIPSLEQLVQQKRVKEEKAARPKFLSKAERARLALERRQKEVEEAKAKQNDKLLDLRK 63
Query: 392 K 394
+
Sbjct: 64 R 64
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 27.1 bits (57), Expect = 3.2
Identities = 23/107 (21%), Positives = 44/107 (41%)
Frame = +2
Query: 104 FQPDSDVHISYEDQQKINKFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEKI 283
FQ + + + + K+++ +D +L QN+V ++ ++E + I
Sbjct: 27 FQEEDQLRRNNKSSNKLSQNEEDAKNMDSVVQKLNELQNNVVAFQKLLQEKT---PLSSI 83
Query: 284 PYLIGEIFICQNLEITLKNLEEAKAKKIHXIHDLEEKCEELKSQMSD 424
L G +NLE + + H ++DL+ E LK Q D
Sbjct: 84 QDLEGFREFMENLEHRYEMTVSEVRRLSHEVNDLQTDRENLKHQFED 130
Score = 25.4 bits (53), Expect = 9.9
Identities = 16/51 (31%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
Frame = +2
Query: 134 YEDQ-QKIN-KFARLNAKVDDYKDELKVKQNDVKNLEEAVEELSLADDSEK 280
+EDQ +K+N + + N+ + KDEL+ LEE + L A E+
Sbjct: 128 FEDQIEKLNSEISNQNSLILQKKDELEKSIQRCSELEEKINSLESAQSIEQ 178
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 26.2 bits (55), Expect = 5.7
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 182 VDDYKDELKVKQNDVKNLEEAVEELSLA 265
++D ++ V QND K + A ELSL+
Sbjct: 343 INDLDHQISVMQNDEKRINNAKTELSLS 370
>SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting
complex subunit Apc4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 719
Score = 26.2 bits (55), Expect = 5.7
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +1
Query: 535 LSYIGFTSNNYCYSKLCCFYTIRIYVS*FENWLTY 639
L+ +G+ NN +S L C + + Y+ F +WL Y
Sbjct: 402 LATLGYLQNNV-FSFLNCLFEEKKYMKHFISWLNY 435
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 26.2 bits (55), Expect = 5.7
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +2
Query: 377 HDLEEKCEELKSQMSDLKAHL 439
+D EE+ L+SQ S LKAHL
Sbjct: 409 NDYEERIHALESQNSALKAHL 429
>SPBC543.09 |||mitochondrial m-AAA protease|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 773
Score = 25.4 bits (53), Expect = 9.9
Identities = 10/27 (37%), Positives = 20/27 (74%)
Frame = +2
Query: 176 AKVDDYKDELKVKQNDVKNLEEAVEEL 256
AK+ +++ ++K+K DV ++EA EE+
Sbjct: 282 AKMFNHETDIKIKFADVAGVDEAKEEI 308
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,686,666
Number of Sequences: 5004
Number of extensions: 46933
Number of successful extensions: 153
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 406444570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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