Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P13_pT_J12
(622 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 107 1e-24
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 97 2e-21
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 89 4e-19
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 85 8e-18
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 77 2e-15
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 77 3e-15
SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|ch... 35 0.011
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 32 0.058
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 29 0.41
SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 27 1.7
SPCC895.04c |ufe1||SNARE Ufe1|Schizosaccharomyces pombe|chr 3|||... 26 3.8
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 5.1
SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosacchar... 25 6.7
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 107 bits (257), Expect = 1e-24
Identities = 59/167 (35%), Positives = 102/167 (61%), Gaps = 1/167 (0%)
Frame = -3
Query: 566 DYLETWQGMQDARQLGLARSIGVSNFNATQITRLV-SNSYIRPVINQIEVNPTNTQEPLV 390
D +E+W+ ++ + G R+IGVSNF I L+ S+ I P +NQIE++P +Q+ +V
Sbjct: 116 DRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVV 175
Query: 389 AHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRG 210
+C+S GI + AY+P + G+ + + L A+A+KY KS QI++RY + RG
Sbjct: 176 DYCESKGIQLAAYAP----LVHGE------KFGNKQLLAIASKYNKSEAQIMIRYCLQRG 225
Query: 209 LIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKNHRVIDISDW 69
I +PKS+ +RI +N D+FDF+++ E++ + ++++ SDW
Sbjct: 226 FIVLPKSSTPRRIKENGDVFDFEISKEDMEKLYNLDEDYH----SDW 268
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 96.7 bits (230), Expect = 2e-21
Identities = 52/156 (33%), Positives = 89/156 (57%), Gaps = 1/156 (0%)
Frame = -3
Query: 560 LETWQGMQDARQLGLARSIGVSNFNATQITRL-VSNSYIRPVINQIEVNPTNTQEPLVAH 384
+ +W+ M++ G RS+GVSN+ + L SN P +NQIE++P +Q+ +V +
Sbjct: 125 IASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKY 184
Query: 383 CQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRGLI 204
CQS IA+ AYSP + R +D L +A K SV Q+L+R+ + +G I
Sbjct: 185 CQSHDIAIEAYSPLTHGI----------RLNDEKLVPIAKKLNISVAQLLIRWSLQKGYI 234
Query: 203 PIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKN 96
PI KST K+ + ++D+F+F + + V ++ F+++
Sbjct: 235 PIIKSTKKEHMLSDLDVFNFTIPDDVVQELSSFDEH 270
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 89.4 bits (212), Expect = 4e-19
Identities = 62/183 (33%), Positives = 93/183 (50%), Gaps = 13/183 (7%)
Frame = -3
Query: 620 YLIHFPIXTK------PDDSPDNIDYL-----ETWQGMQDARQLGLARSIGVSNFNATQI 474
YLIH+P+ K P D N+ Y ETW+ M+ + G R IG+SNFN T +
Sbjct: 115 YLIHWPVSFKTGEDKFPKDKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNL 174
Query: 473 TRLVSNSYIRPVINQIEVNPTNTQEPLVAHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRS 294
R++ + ++P ++Q+E++P Q V + LGI V AYSPFG + ++ P
Sbjct: 175 ERILKVAKVKPAVHQMELHPFLPQTEFVEKHKKLGIHVTAYSPFGNQNTIYESKI-PKLI 233
Query: 293 DDPTLTALANKYRKSV--GQILLRYLIDRGLIPIPKSTNKQRIAQNIDLFDFQLTFEEVA 120
+ T+ +A + V I + + I RG IPKS N+QRI N LT E++
Sbjct: 234 EHETIQKIAKSKGEGVTGATIAVSWAITRGTSVIPKSVNEQRIKSNFKY--IPLTKEDMD 291
Query: 119 AIN 111
IN
Sbjct: 292 EIN 294
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 85.0 bits (201), Expect = 8e-18
Identities = 57/178 (32%), Positives = 86/178 (48%), Gaps = 2/178 (1%)
Frame = -3
Query: 620 YLIHFPIXTKPDDSPDNIDYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRP 441
YL+H PI P + E W+ M+ A GL S+GVSNF + L+ S I P
Sbjct: 106 YLLHSPIPFYEKKIPIS----EGWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITP 161
Query: 440 VINQIEVNPT--NTQEPLVAHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALA 267
+NQIE +P +PLV CQS GI V Y P +V Q P ++ +L
Sbjct: 162 RVNQIEFHPQVYKAAKPLVEFCQSKGIIVEGYGPLSPLVRDAQ----GPVAE--FTKSLE 215
Query: 266 NKYRKSVGQILLRYLIDRGLIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKNH 93
+KY S QILL++ +G+IPI ++ +R+ + ++ F L ++ + H
Sbjct: 216 SKYHVSDTQILLKWAYSKGVIPITTTSKIERMKECLNFDSFTLDKADIDELGTLGVQH 273
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 77.4 bits (182), Expect = 2e-15
Identities = 56/183 (30%), Positives = 91/183 (49%), Gaps = 13/183 (7%)
Frame = -3
Query: 620 YLIHFP--IXTKPDDSPDN---------IDYLETWQGMQDARQLGLARSIGVSNFNATQI 474
YLIH+P + + P++ P N + ETWQ M++ + G R IG+SNFN +
Sbjct: 114 YLIHWPFALLSGPEELPRNEKGELIYEDVPIEETWQAMEELLETGKVRYIGISNFNNEYL 173
Query: 473 TRLVSNSYIRPVINQIEVNPTNTQEPLVAHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRS 294
R++ + ++P I+Q+E++P Q + + L I V AYSP +
Sbjct: 174 DRVLKIAKVKPTIHQMELHPYLPQTEYLEKHKKLQIHVSAYSPLA-NQNDAYNSDISKLI 232
Query: 293 DDPTLTALANKYRKSV--GQILLRYLIDRGLIPIPKSTNKQRIAQNIDLFDFQLTFEEVA 120
+ TL +AN + + I + + + RG +PKS N+ RI N L+ LT +E+
Sbjct: 233 EHKTLVDIANARGEGITPANIAISWAVKRGTSVLPKSVNESRIVSNF-LY-IPLTDKEME 290
Query: 119 AIN 111
AIN
Sbjct: 291 AIN 293
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 76.6 bits (180), Expect = 3e-15
Identities = 45/141 (31%), Positives = 75/141 (53%)
Frame = -3
Query: 560 LETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEVNPTNTQEPLVAHC 381
+E+W+ + ++ G IGVSN+N + ++S P INQ+E + N + +++C
Sbjct: 119 IESWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYC 178
Query: 380 QSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRGLIP 201
+ GI V A+SP ++RG R D L L+ KY K+ ILLRY + +G+ P
Sbjct: 179 FNHGILVQAFSP----LTRGY------RLSDIRLLDLSLKYNKTPANILLRYCLQKGVSP 228
Query: 200 IPKSTNKQRIAQNIDLFDFQL 138
I K+++ I +N+ F L
Sbjct: 229 IFKASSFVHIHENVKAEQFML 249
>SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 333
Score = 34.7 bits (76), Expect = 0.011
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
Frame = -3
Query: 272 LANKYRKSVGQILLRYLIDRG---LIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFN 102
LA K+ S+ + L ++I G +IPIP ST QR N+ L+ E++ +
Sbjct: 254 LAQKFGMSMPEFALNFIIANGKGMIIPIPGSTTVQRAESNLSALKKSLSSEQLEEAKKVL 313
Query: 101 KNHRVIDI 78
H++ +
Sbjct: 314 DKHQIFGL 321
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 32.3 bits (70), Expect = 0.058
Identities = 13/48 (27%), Positives = 28/48 (58%)
Frame = -3
Query: 563 YLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEV 420
+++TW+ +++ G ++GVS F ++ RL+S+ + P QI +
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQINI 205
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 29.5 bits (63), Expect = 0.41
Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 6/82 (7%)
Frame = -3
Query: 281 LTALANKYRKSVGQILLRYLID--RGLI---PIPKSTNKQRIAQNIDLFDFQLTFEEVAA 117
L+AL KS + YL++ RG I P+P T K++ A + + F+ F +
Sbjct: 2189 LSALVQIIEKSSSYEICNYLLEIVRGWIMNSPVPVPTVKEKAALLLKMVTFEGRFSQNEQ 2248
Query: 116 INQFNKNHRVI-DISDWKDYPN 54
+ FNK I DI + + Y N
Sbjct: 2249 NDLFNKYLSFIADIYEMEPYKN 2270
>SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 708
Score = 27.5 bits (58), Expect = 1.7
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Frame = +3
Query: 411 CRIHLNLVNNRPDVTVAD---QSRDLRSVEVGY-SNGP 512
CR NL+N P++TVA ++ DL+ V SNGP
Sbjct: 443 CRFRRNLINLLPEITVAHFEAEALDLKFVAKSLPSNGP 480
>SPCC895.04c |ufe1||SNARE Ufe1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 319
Score = 26.2 bits (55), Expect = 3.8
Identities = 14/61 (22%), Positives = 26/61 (42%)
Frame = +3
Query: 93 MILVKLVNGRYLFKRQLEIE*IDVLSDPLLVCGFRNRD*TSVDQISE*NLSNTLPVFISE 272
++ +K + G YL R + LS PL+ C +D++ + + + IS
Sbjct: 48 LVFLKKIRGAYLKDRTFSN--VQRLSKPLMECSLEELSKLELDEVQRDEIEHEVSSAISS 105
Query: 273 C 275
C
Sbjct: 106 C 106
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 25.8 bits (54), Expect = 5.1
Identities = 24/87 (27%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
Frame = -3
Query: 506 IGVSNFNATQI-TRLVSNSY-IRP-VINQIEVNPTNTQEPLVAHCQSLGIAVMAYSPFGF 336
+ SNF+A+ I RL S + I P V+ Q +V+ + + + QS +++ FG
Sbjct: 309 VSKSNFHASNIGNRLFSAALSIFPLVLLQSDVSAVQSSLLIGLYLQSTIYEKSSFAYFGL 368
Query: 335 VVSRGQTGAPPPRSDDPTLTALANKYR 255
+ SDDP+LT + + R
Sbjct: 369 AIKFAVALGLHKNSDDPSLTQNSKELR 395
>SPBC1718.07c |zfs1|moc4|transcription factor Zfs1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 404
Score = 25.4 bits (53), Expect = 6.7
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 317 SDHATPRTRTDCTPSRRYRDSGSVPLAALVCL*DSPQS 430
S+H +P + + PSR SGS+ + L L D +S
Sbjct: 147 SNHPSPISSLESLPSRSSTGSGSLDFSGLANLHDDSKS 184
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,673,133
Number of Sequences: 5004
Number of extensions: 56115
Number of successful extensions: 177
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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