Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P13_F_P03
         (817 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa...    97   2e-21
SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc...    92   7e-20
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ...    28   1.4  
SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc...    27   3.2  
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar...    27   4.2  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    26   5.6  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    26   5.6  
SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone oxidase|Schizosacch...    26   7.4  
SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces ...    25   9.7  

>SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase
           Uch1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 222

 Score = 97.5 bits (232), Expect = 2e-21
 Identities = 67/211 (31%), Positives = 107/211 (50%), Gaps = 6/211 (2%)
 Frame = +2

Query: 158 PLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKK 337
           PLE+ P+VL  +LQK+GV +  ++ D+  L+ E   ++PRPV +++ +FP S     +K 
Sbjct: 4   PLENTPEVLEPYLQKIGVQDA-SVFDLFSLE-EIPEYIPRPVHALLFVFPSSGTKTIYKG 61

Query: 338 TEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDG-HMQKFLNEAK 514
           +    IL K    S  + +  Q I NACGTI L+H+V+N     ++++   ++  +  A+
Sbjct: 62  SR---ILPKD---SDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAE 115

Query: 515 GLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAED-PVNHHFISFV----HKDGALYE 679
           G     R KL+E S+ +   H   A        S ED   + HFI FV      D   YE
Sbjct: 116 GSSIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYE 175

Query: 680 LDGRKAFPVNHGPTSQETLLEDAXKICKEFM 772
           LDGR+  PV H     + L  +   + K ++
Sbjct: 176 LDGRQEGPVQHSEIESDLLNAEVLSVIKNYI 206


>SPBC409.06 |uch2||ubiquitin C-terminal hydrolase
           Uch2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 300

 Score = 92.3 bits (219), Expect = 7e-20
 Identities = 64/224 (28%), Positives = 114/224 (50%), Gaps = 6/224 (2%)
 Frame = +2

Query: 161 LESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKT 340
           +ES+  V    ++ LGV +   + ++  LD ++L   P  +  ++ LF  +   +    T
Sbjct: 6   IESDAGVFTDLIENLGVKDV-EVDELYSLDVDSLRQFP-DIYGIIFLFKWNSKVDKPDGT 63

Query: 341 EENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEAKGL 520
            + + +        NIF+ KQ I+NAC T AL+  + N++D I+L    + +F + +K L
Sbjct: 64  MDYDSMD-------NIFFAKQVINNACATQALLSVLLNHSDEIDLGTT-LSEFKDFSKTL 115

Query: 521 DATARGKLLEKSEGIINAHKELAQEG-----QTNTPSAEDPVNHHFISFVHKDGALYELD 685
               +G+ L  SE I   H   A+       +    + ED V +HFI++ + +   YELD
Sbjct: 116 PPELKGEALGNSEHIRCCHNSFARSDPFISEEVRAATDEDEV-YHFIAYTNINNVFYELD 174

Query: 686 GRKAFPVNHGPTSQETLLEDAXKICKEFMAR-DPNEVRFTVIAL 814
           G +A P+NHG  ++E   E A  + +  +A  DP E+RF ++ +
Sbjct: 175 GLQAAPINHGSCTKEEFAEKAVSVIQARIANYDPAEIRFNLMVI 218


>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 639

 Score = 28.3 bits (60), Expect = 1.4
 Identities = 17/62 (27%), Positives = 28/62 (45%)
 Frame = +2

Query: 554 SEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFPVNHGPTSQET 733
           S  ++    ELA +  TNT      V   ++++V  +   + L   K F   H P+S E 
Sbjct: 126 SRALLEQFAELASK--TNTSHMIHQVYDQYLNYVVLESDFFSLQLPKIFHTFHNPSSDEA 183

Query: 734 LL 739
           L+
Sbjct: 184 LI 185


>SPBC23E6.07c |rfc1||DNA replication factor C complex subunit
           Rfc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 934

 Score = 27.1 bits (57), Expect = 3.2
 Identities = 14/54 (25%), Positives = 23/54 (42%)
 Frame = +2

Query: 542 LLEKSEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVHKDGALYELDGRKAFP 703
           L +  +G I   K  A + +    +  + ++H   S VH+D  L   DG    P
Sbjct: 41  LSDDEDGTIENSKVPASKSKVQKRNESEDISHSLPSIVHEDDKLVGSDGVSTTP 94


>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 580

 Score = 26.6 bits (56), Expect = 4.2
 Identities = 14/36 (38%), Positives = 16/36 (44%)
 Frame = +3

Query: 660 KMEHCMS*MAVKLFLSIMAQLHRKLCWKMXQKFAKN 767
           +M HC S     L   I   L R L W+  QK A N
Sbjct: 508 RMSHCSSNSNKVLASEISESLRRDLLWERRQKAAMN 543


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
            Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +2

Query: 617  AEDPVNHHFISFVHKDGALYELDGR 691
            AE+ V  HF+    KD  LY++D R
Sbjct: 1099 AEESVKRHFLRLWLKDNGLYDVDIR 1123


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 26.2 bits (55), Expect = 5.6
 Identities = 9/28 (32%), Positives = 20/28 (71%)
 Frame = +2

Query: 566 INAHKELAQEGQTNTPSAEDPVNHHFIS 649
           ++A + L ++ +++T + E+PV+HH  S
Sbjct: 337 LSARRALFEKKESSTKNVENPVSHHLKS 364


>SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone
           oxidase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 461

 Score = 25.8 bits (54), Expect = 7.4
 Identities = 18/55 (32%), Positives = 25/55 (45%), Gaps = 8/55 (14%)
 Frame = +2

Query: 131 TEMATETLVPLESNPDVLNKFLQ-------KLGVPNKWNI-VDVMGLDPETLSWV 271
           ++  +E  +PLES PD L K +        K+G    W I V V    PE   W+
Sbjct: 318 SQHVSEWGIPLESAPDALEKLINYTVDDAGKIGAYTHWPIEVRVCAPTPEDECWL 372


>SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 296

 Score = 25.4 bits (53), Expect = 9.7
 Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
 Frame = +2

Query: 452 NNTDIIELSDGHMQ-KFLNEAKGLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAEDP 628
           +NT+++   +  ++ K ++  K +D +  G  L + E    +H +    GQ      +DP
Sbjct: 31  SNTNMLSTEENIVKAKAVSRKKPVDVSYAG--LARHE----SHVDTDYHGQALNTDNQDP 84

Query: 629 VNHHFISFVHKDGALYE 679
            N+   SFV K+  LYE
Sbjct: 85  KNYQARSFV-KENELYE 100


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,369,510
Number of Sequences: 5004
Number of extensions: 69977
Number of successful extensions: 220
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 216
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)