Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P13_F_N10
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 129 6e-31
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 89 7e-19
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 71 2e-13
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 65 1e-11
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 61 2e-10
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 5e-09
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 44 4e-05
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 39 0.001
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 38 0.002
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 29 0.63
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 28 1.9
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 28 1.9
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 28 1.9
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 5.9
SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyce... 26 7.8
SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 7.8
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 129 bits (311), Expect = 6e-31
Identities = 69/178 (38%), Positives = 106/178 (59%), Gaps = 3/178 (1%)
Frame = +1
Query: 151 LSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQ 330
++K +I+ + ++V+FYAPWCGHCK+LAPEY AA +L E+ I L +VD T+E
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEG 84
Query: 331 DLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKELI 507
DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P V+ S + + +
Sbjct: 85 DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL-LPTVKPISKDTLENFV 143
Query: 508 D-ANTVIVFGFFSDQSSTRAKTFLSTAQVV-DDQVFAIVSDEKVIKELEAEDEDVVLF 675
+ A+ + V FF DQ T+ A+V+ DD VFA D+++ K L + +V F
Sbjct: 144 EKADDLAVVAFFKDQKLN--DTYTEVAEVMKDDFVFAASDDKELAKSLGSNFPGIVAF 199
Score = 91.9 bits (218), Expect = 1e-19
Identities = 45/111 (40%), Positives = 75/111 (67%), Gaps = 4/111 (3%)
Frame = +1
Query: 130 TEENVLVLSKANFETVI-STTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 306
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 307 KVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 450
K+DAT E D+ S + G+PT+ FF+ +P+ Y G R +D+ +++ K
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 89.0 bits (211), Expect = 7e-19
Identities = 50/150 (33%), Positives = 78/150 (52%), Gaps = 2/150 (1%)
Frame = +1
Query: 73 VLIFTAIALLGLALGDEVPTEENVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPE 252
+L F AL L V +++ L T+ ++ + L+EFYA WCGHCKSLAP
Sbjct: 5 LLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKSLAPV 60
Query: 253 YAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQAD 426
Y + L E+ + + + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D
Sbjct: 61 YEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVD 119
Query: 427 DIISWLKKKTGPPAVEVTSAEQAKELIDAN 516
+ ++ +KTG ++ EL N
Sbjct: 120 SLTQFVSEKTGIKKRKIVLPSNVVELDSLN 149
Score = 79.4 bits (187), Expect = 6e-16
Identities = 40/111 (36%), Positives = 63/111 (56%), Gaps = 4/111 (3%)
Frame = +1
Query: 139 NVLVLSKANFETVISTTEY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVD 315
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y + K+ + E +++ K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNVEIVKIN 199
Query: 316 ATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKTG 459
A D+ + V +PT+KFF P Y G R + +I ++ KK+G
Sbjct: 200 ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 70.9 bits (166), Expect = 2e-13
Identities = 43/144 (29%), Positives = 65/144 (45%), Gaps = 6/144 (4%)
Frame = +1
Query: 76 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEY 255
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 256 AKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGR 417
K A+ L P+ DA Q + + Y V+G+PT+K GS + DY+G R
Sbjct: 71 QKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129
Query: 418 QADDIISWLKKKTGPPAVEVTSAE 489
+ ++ P V++ ++E
Sbjct: 130 SYKSLQKFVSDSI-PSKVKILTSE 152
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 65.3 bits (152), Expect = 1e-11
Identities = 38/121 (31%), Positives = 64/121 (52%), Gaps = 4/121 (3%)
Frame = +1
Query: 148 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 327
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 328 QDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAVEVTSAEQA 495
+ +A GV+ PT FF NG ID G QA + + K TG A+ +S+
Sbjct: 64 RQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGALASSSSAPV 123
Query: 496 K 498
K
Sbjct: 124 K 124
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 61.3 bits (142), Expect = 2e-10
Identities = 35/101 (34%), Positives = 49/101 (48%)
Frame = +1
Query: 148 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 327
V S ++ T IS + +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 328 QDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 450
D+A+ GV PT+ FR G +D G + S L K
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 56.4 bits (130), Expect = 5e-09
Identities = 24/85 (28%), Positives = 46/85 (54%)
Frame = +1
Query: 148 VLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 327
V + F++++ + ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 328 QDLAESYGVRGYPTLKFFRNGSPID 402
++A GV P+ ++NG I+
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 43.6 bits (98), Expect = 4e-05
Identities = 26/143 (18%), Positives = 62/143 (43%)
Frame = +1
Query: 127 PTEENVLVLSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 306
PT + + A+ + ++ E ++FY+ C C ++ + A ++ + +A
Sbjct: 278 PTGTSKALALDADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRM---RGKLNVA 334
Query: 307 KVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVEVTSA 486
++ + + Y ++ +PT FF+ + ++Y G D++S+ ++ EV
Sbjct: 335 HINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIREVELL 394
Query: 487 EQAKELIDANTVIVFGFFSDQSS 555
+ + N + F +F D S
Sbjct: 395 DTVN--AEKNGDVFFLYFYDDDS 415
Score = 42.7 bits (96), Expect = 6e-05
Identities = 25/86 (29%), Positives = 43/86 (50%), Gaps = 2/86 (2%)
Frame = +1
Query: 151 LSKANFETVISTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAE--EESPIKLAKVDATQ 324
L+ + E+ +S + +++Y P CG CK L P + K E E S +VD ++
Sbjct: 31 LTDNDLESEVSKGTWF-IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89
Query: 325 EQDLAESYGVRGYPTLKFFRNGSPID 402
E L+ +R PTL ++NG ++
Sbjct: 90 E--LSSCANIRAVPTLYLYQNGEIVE 113
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 38.7 bits (86), Expect = 0.001
Identities = 22/104 (21%), Positives = 43/104 (41%)
Frame = +1
Query: 190 EYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPT 369
+ IL+ FYAPW CK + + + A++ K++A + D+AES+ V P
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVPL 76
Query: 370 LKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKE 501
+ G + + + + P +++S + E
Sbjct: 77 FVLIHGAKVLARISGANPQKLKAAIDEYIQPLISQISSTNASVE 120
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 37.5 bits (83), Expect = 0.002
Identities = 15/87 (17%), Positives = 43/87 (49%)
Frame = +1
Query: 229 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 408
HC+ A ++ + +K+A+V+ +E+++ + ++ +PT + F+ I Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267
Query: 409 GGRQADDIISWLKKKTGPPAVEVTSAE 489
G + ++S+ + A+++ +
Sbjct: 268 GPLKYQQLLSYSNQVASYQAIKIEEGD 294
Score = 28.3 bits (60), Expect = 1.5
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +1
Query: 334 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 480
LA YG + P++ RNG PI Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 29.5 bits (63), Expect = 0.63
Identities = 27/86 (31%), Positives = 40/86 (46%)
Frame = +1
Query: 229 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 408
+C L P +AA L + +SP L VDA + L S+GVR + G+ +
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVRKAVIITLGSQGAYYKSA 240
Query: 409 GGRQADDIISWLKKKTGPPAVEVTSA 486
G A ++S K K AV+ T+A
Sbjct: 241 NGESA--LVSACKVK----AVDTTAA 260
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 27.9 bits (59), Expect = 1.9
Identities = 29/124 (23%), Positives = 53/124 (42%), Gaps = 8/124 (6%)
Frame = +1
Query: 217 PWCG-HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGS 393
PW + ++L + + KL E + K D + L G++ Y ++ F N
Sbjct: 226 PWKNPNTENLTSQVSIKEPKLYEPHPTTAIKKADGKIIRILVIDVGMK-YNQIRCFLNRG 284
Query: 394 ------PIDYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGF-FSDQS 552
P DY ++ D + ++ G P++ ++ K ++++ TV VFG F Q
Sbjct: 285 VELLVVPWDYDFTKETYDGL-FISNGPGDPSLMDLVVDRVKRVLESKTVPVFGICFGHQI 343
Query: 553 STRA 564
RA
Sbjct: 344 MARA 347
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 27.9 bits (59), Expect = 1.9
Identities = 18/70 (25%), Positives = 31/70 (44%), Gaps = 1/70 (1%)
Frame = +1
Query: 409 GGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGFFSD-QSSTRAKTFLSTA 585
GGR D + +K+G V E +E+ + + F S+ +S T K + T
Sbjct: 186 GGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTY 245
Query: 586 QVVDDQVFAI 615
Q+ D+Q +
Sbjct: 246 QLPDNQTITL 255
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 27.9 bits (59), Expect = 1.9
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -1
Query: 575 RKVLALVELWSEKKPNTITVLASISSLACSAEVTSTA 465
+K +L + W + + LASISS A SA + ST+
Sbjct: 673 KKAASLPQFWLSPRSHNTARLASISSFAKSAVMNSTS 709
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -1
Query: 320 VASTFASLIGDSSSASFVAALAYSGARDLQW 228
V++ F SL+G S FVA + R L+W
Sbjct: 1039 VSNIFKSLVGSMSCVEFVAEARETINRSLEW 1069
>SPBC20F10.08c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 747
Score = 25.8 bits (54), Expect = 7.8
Identities = 9/12 (75%), Positives = 11/12 (91%)
Frame = -3
Query: 75 NTHCDIIGTFLL 40
NTHCDI+ +FLL
Sbjct: 275 NTHCDIVTSFLL 286
>SPAC644.16 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 422
Score = 25.8 bits (54), Expect = 7.8
Identities = 30/136 (22%), Positives = 50/136 (36%), Gaps = 6/136 (4%)
Frame = +1
Query: 241 LAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQ 420
++P Y A A S + +T RG+PT + N +P Y
Sbjct: 271 VSPMYGSHAAPYASTPSAAVGSSRGSTPASATVPISPARGFPTTSAY-NPAPPAYGMANP 329
Query: 421 A---DDIISWLKKKTGP---PAVEVTSAEQAKELIDANTVIVFGFFSDQSSTRAKTFLST 582
A I S +G P++ TSA+ N + D+++T+A
Sbjct: 330 AYGSTGIRSSSIPSSGSIRSPSLTTTSAQATTNA--TNNITTTTAAQDENATKAALIAQL 387
Query: 583 AQVVDDQVFAIVSDEK 630
+ DDQ+ + D+K
Sbjct: 388 MALTDDQINVLPPDQK 403
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,130,289
Number of Sequences: 5004
Number of extensions: 57775
Number of successful extensions: 183
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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