Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P13_F_M08
(819 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC16E8.17c |||succinate-CoA ligase alpha subunit|Schizosacchar... 320 2e-88
SPBC1703.07 |||ATP citrate synthase subunit 1 |Schizosaccharomyc... 63 5e-11
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 30 0.45
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 28 1.8
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 27 2.4
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 26 5.6
SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter Itr1|Schizo... 26 7.4
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 9.8
SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protei... 25 9.8
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 9.8
>SPAC16E8.17c |||succinate-CoA ligase alpha
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 331
Score = 320 bits (785), Expect = 2e-88
Identities = 154/241 (63%), Positives = 183/241 (75%)
Frame = +1
Query: 97 LSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKV 276
L +F +L N S + Y +T NL++ S+TKVI QGFTGKQGTFH+Q A+DYGTKV
Sbjct: 12 LRRFSSSSQLKN----SKSLYEQTIPNLMINSDTKVIFQGFTGKQGTFHAQHAMDYGTKV 67
Query: 277 VGGVSPKKAGTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVC 456
VGG +PKKAGT HLGKPVFGT++EA T A AS ++VPPP +PLIV
Sbjct: 68 VGGTNPKKAGTTHLGKPVFGTIEEAMKETKADASAVFVPPPLAAGAIEEAIAAEVPLIVA 127
Query: 457 ITEGVPQHDMVRVKHALLRQNKSRLVGPNCPGIIAPEKCKIGIMPAAVHKRGCIGVVSRS 636
ITEG+PQHDM+RV L Q+KSRLVGPNCPGII P +CKIGIMP+ +HK GCIG+VSRS
Sbjct: 128 ITEGIPQHDMLRVSDILKTQSKSRLVGPNCPGIIRPGQCKIGIMPSHIHKPGCIGIVSRS 187
Query: 637 GTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFIDCLXVFLKDPXTKGIILIGEIGGNA 816
GTLTYEA +QTT T LGQ+L +GIGGDPF GT+FID L +FL DP T+GIILIGEIGG+A
Sbjct: 188 GTLTYEAVNQTTQTDLGQSLVIGIGGDPFPGTNFIDALKLFLDDPNTQGIILIGEIGGSA 247
Query: 817 E 819
E
Sbjct: 248 E 248
>SPBC1703.07 |||ATP citrate synthase subunit 1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 615
Score = 62.9 bits (146), Expect = 5e-11
Identities = 56/185 (30%), Positives = 84/185 (45%), Gaps = 13/185 (7%)
Frame = +1
Query: 304 GTEHLGKPVFGTVKEAKAGTGATASVIYVPPPGXXXXXXXXXXXXMPLIVCIT---EGVP 474
GT+ + PV+ T++EA V+ P I CI EGVP
Sbjct: 64 GTKEILLPVYRTIEEA-CTKHPEVDVVVNFASSRSAYASTMELMEFPQIRCIAIIAEGVP 122
Query: 475 QHDMVRVKHALLRQNKSRLV--GPNCPGIIAPEKCKIG--------IMPAAVHKRGCIGV 624
+ R + L+ + +V GP G I P KIG I+ + +++ G +
Sbjct: 123 ER---RAREILVTSKEKNVVIIGPATVGGIKPGCFKIGNTGGMMDNIVASKLYRPGSVAY 179
Query: 625 VSRSGTLTYEACHQTTITGLGQTLCVGIGGDPFNGTDFIDCLXVFLKDPXTKGIILIGEI 804
VS+SG ++ E + + T G + IGGD + GT FID L F DP K ++L+GE+
Sbjct: 180 VSKSGGMSNELNNIISHTTDGVYEGIAIGGDRYPGTTFIDHLIRFEADPACKLMVLLGEV 239
Query: 805 GGNAE 819
GG E
Sbjct: 240 GGVEE 244
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 29.9 bits (64), Expect = 0.45
Identities = 25/93 (26%), Positives = 41/93 (44%)
Frame = +2
Query: 539 LTVLVSLLPRSVKLVSCQLQCTNVVASELYRVLGH*PMKPVIRLLLLA*AKLCVSVLEGI 718
LT + L R VS C N V S + + ++ LL L + L ++ +G
Sbjct: 102 LTTIAGRLDRFRSTVSVSFDCLNWVNSMIPNLPEKELQYWILELLPLQSSFLSYALRDGK 161
Query: 719 PSMEQTSLTAXKFSSRILXPRASSSLVKLVEML 817
PS+ +++ + + R L + SL KLV L
Sbjct: 162 PSVADSAIKSTRRCYRSLFCKKMDSLKKLVSFL 194
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 27.9 bits (59), Expect = 1.8
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = -1
Query: 576 FTLLGSNDTRTVRTNQSRLVLSQEGMFYSHHIMLWHTFCNAHNEGHFSLN 427
+TLL N T +R +QS + G + H W T N N + S N
Sbjct: 186 YTLLLQNTTVVIRRDQSVVYTGDVGTNFFHSPTKWITHSNVSNGYYTSRN 235
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 27.5 bits (58), Expect = 2.4
Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
Frame = +1
Query: 154 PYAETRKNLILTSETKVIVQGFTGKQGTFHSQQALDYGTKVVG-GVSPKKAGTEHLGKPV 330
PY+ R NL +TS F G + H Q+ + ++ + PK+ + KPV
Sbjct: 470 PYSTFRLNLSVTSPYNA---DFDGDEMNMHVPQSEETRAEIQEITMVPKQIVSPQSNKPV 526
Query: 331 FGTVKEAKAG 360
G V++ AG
Sbjct: 527 MGIVQDTLAG 536
>SPCC1235.05c |fft2||fun thirty related protein
Fft2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1284
Score = 26.2 bits (55), Expect = 5.6
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -2
Query: 365 PVPAFASLTVPNTGLPRCSVPAFFGDTPPTTLVP 264
P+P++ + ++PN G+P ++P P T +P
Sbjct: 124 PLPSYNTASLPNAGIP-AAMPGMPSGYPGTVPIP 156
>SPAC4F8.15 |itr1|SPAC7D4.01|myo-inositol transporter
Itr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 25.8 bits (54), Expect = 7.4
Identities = 12/41 (29%), Positives = 19/41 (46%), Gaps = 1/41 (2%)
Frame = +1
Query: 43 PFRRFTFNNSTMAMPVRVLSKFKDGLK-LGNVRFASGNPYA 162
PF F N T +PV +S+ +G + N + P+A
Sbjct: 33 PFESSVFENKTQVLPVDSVSRLSNGARSRSNSNISLSEPHA 73
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 25.4 bits (53), Expect = 9.8
Identities = 13/69 (18%), Positives = 32/69 (46%)
Frame = +1
Query: 55 FTFNNSTMAMPVRVLSKFKDGLKLGNVRFASGNPYAETRKNLILTSETKVIVQGFTGKQG 234
+ NN+ V+V F+ +KLG V ++T++N +++ ++ F +
Sbjct: 801 YKLNNTPSEQSVKVSHFFEFNMKLGEVTLILCREDSQTKRNSMVSVNFAKLLLSFNQFED 860
Query: 235 TFHSQQALD 261
H + +++
Sbjct: 861 NSHLRMSIN 869
>SPBC582.06c |mcp6|hrs1, mug3|meiosis specific coiled-coil protein
Mcp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 327
Score = 25.4 bits (53), Expect = 9.8
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -2
Query: 140 NRTLPSFSPSLNLERTRTGIAIVELLNVNL 51
NR PS+S SLN T ++ VE +N +L
Sbjct: 297 NRAPPSYSFSLNCSHTYQPVSCVEPVNHDL 326
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 25.4 bits (53), Expect = 9.8
Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
Frame = -1
Query: 360 ACLCLFDCTKHRLTKMFCTSLLW*HS---SNNFSTIVKGLLTVEGTLLSSETLNNHFCFT 190
A L L +++ +F SL H+ NF+ I+ + ++ S+ NHF F
Sbjct: 632 AALDLISLIVNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYFL 691
Query: 189 CKDQI 175
CK I
Sbjct: 692 CKGDI 696
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,398,973
Number of Sequences: 5004
Number of extensions: 71268
Number of successful extensions: 196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 400438000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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