Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P13_F_M06
(803 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 246 3e-66
SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual 108 1e-24
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 72 8e-14
SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom... 26 5.5
SPCC285.03 |||ATP-dependent RNA helicase Dbp6|Schizosaccharomyce... 26 7.2
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 9.5
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.5
SPAC25B8.03 |||phosphatidylserine decarboxylase|Schizosaccharomy... 25 9.5
SPBC30D10.14 |||dienelactone hydrolase family|Schizosaccharomyce... 25 9.5
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 246 bits (602), Expect = 3e-66
Identities = 116/245 (47%), Positives = 161/245 (65%), Gaps = 1/245 (0%)
Frame = +3
Query: 66 SITVLKQAPMLXXGFNLALIQLSVGPNKSKHXAQAVKEIHLAKXXGAQLVALPECFNSPY 245
S++ P F + L+QL+ +KS++ A ++ A G+ ++ LPE FNSPY
Sbjct: 30 SVSASSLVPKDFRAFRIGLVQLANTKDKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPY 89
Query: 246 GTKYFDEYAEEVPSGETS-RALSKXXXXXXXXXXXXXXPERYEKKLYNTCTVWDDTGKLL 422
GT YF++YAE + S +ALS PER + KLYNT V+D +GKL+
Sbjct: 90 GTGYFNQYAEPIEESSPSYQALSSMAKDTKTYLFGGSIPERKDGKLYNTAMVFDPSGKLI 149
Query: 423 AQHRKMHLFDIDIPNKITFKESEVLSAGDKITSFDFLGSKIGIGICYDLRFPEMAHLMAK 602
A HRK+HLFDIDIP ++F+ES+ LS GD +T D K G+GICYD+RFPE+A + A+
Sbjct: 150 AVHRKIHLFDIDIPGGVSFRESDSLSPGDAMTMVDTEYGKFGLGICYDIRFPELAMIAAR 209
Query: 603 EGCSLLIYPGAFNMTTGPRHWELLGRARATDXQLWVALVSPARDSAAGYVAWGHSLLVXP 782
GCS++IYPGAFN++TGP HWELL RARA D +++VA +PARD A Y +WGHS +V P
Sbjct: 210 NGCSVMIYPGAFNLSTGPLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVDP 269
Query: 783 WGQVV 797
+G+V+
Sbjct: 270 FGKVI 274
>SPBC651.02 |||nitrilase |Schizosaccharomyces pombe|chr 2|||Manual
Length = 276
Score = 108 bits (259), Expect = 1e-24
Identities = 57/152 (37%), Positives = 84/152 (55%), Gaps = 3/152 (1%)
Frame = +3
Query: 357 PERYEKKLYNTCTVWDDT-GKLLAQHRKMHLFDIDIPNKITFKESEVLSAGDKITS--FD 527
P + + KL N+ + G++++++ K HLFD++I N T KES G+ I
Sbjct: 87 PSKVKNKLLNSSLFIEPLHGEIISRYSKAHLFDVEIKNGPTLKESNTTLRGEAILPPCKT 146
Query: 528 FLGSKIGIGICYDLRFPEMAHLMAKEGCSLLIYPGAFNMTTGPRHWELLGRARATDXQLW 707
LG K+G IC+D+RFPE A + G ++ YP AF TG HWE+L RARA D Q +
Sbjct: 147 PLG-KVGSAICFDIRFPEQAIKLRNMGAHIITYPSAFTEKTGAAHWEVLLRARALDSQCY 205
Query: 708 VALVSPARDSAAGYVAWGHSLLVXPWGQVVEQ 803
V + ++GHS++V PWG V+ Q
Sbjct: 206 VIAPAQGGKHNEKRASYGHSMIVDPWGTVIAQ 237
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 72.1 bits (169), Expect = 8e-14
Identities = 63/238 (26%), Positives = 105/238 (44%), Gaps = 8/238 (3%)
Frame = +3
Query: 111 NLALIQLSVGPNKSKHXAQAVKE-IH--LAKXXGAQLVALPECFNSPYGT-KYFDEYAEE 278
N+A +Q++ KH Q + +H + L+ PE S Y F + AE
Sbjct: 4 NIACVQMAPKVCDVKHNLQKMSSYVHEVMESNPSTNLILFPELITSGYECGNTFTQIAEI 63
Query: 279 VPSGETSRALSKXXXXXXXXXXXXXXPERYEKK---LYNTCTVWDDTGKLLAQHRKMHLF 449
G + + +S PE+ EK+ +YN+C + G L +RK+HLF
Sbjct: 64 AGEGPSFKTMSNLAAKYHVNIIYGF-PEKEEKQSNIIYNSCIYITENGNLGGVYRKVHLF 122
Query: 450 DIDIPNKITFKESEVLSAGDKITSFDFLGSKIGIGICYDLRFPEMAHLMAKEGCSLLIYP 629
D + + FK+ G F+ K+G+ IC+D FPE+A + A G LL+
Sbjct: 123 DTERKH---FKK------GSDFPIFETSFGKLGVMICWDTAFPEVARIHALNGADLLVVA 173
Query: 630 GAFNMTTGPRHWELLGRARATDXQL-WVALVSPARDSAAGYVAWGHSLLVXPWGQVVE 800
+ W+L+ +ARA + + VA D + +GHS ++ P G+V++
Sbjct: 174 TNWENPYSD-DWDLVTKARAFENCIPLVAANRVGTDEKLSF--FGHSKIIGPTGKVIK 228
>SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 550
Score = 26.2 bits (55), Expect = 5.5
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +1
Query: 253 SILMNMQRKCHRGRRAELYPKPLQKLECA*LEVP 354
S L N+ R +RA + +QKL C L+ P
Sbjct: 356 STLRNLAASSERNKRAIIEANAIQKLRCLILDAP 389
>SPCC285.03 |||ATP-dependent RNA helicase Dbp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 604
Score = 25.8 bits (54), Expect = 7.2
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
Frame = -2
Query: 520 DVILSPAERTSDSLN-VILLGMSMSKRCI-FLCCAKSFPVSSQTVQVLYNFFSYLSG 356
DV +S A + +L+ VI + +S R + L C P TVQV F Y+SG
Sbjct: 175 DVCVSAATGSGKTLSYVIPIVQCLSHRTVPRLRCVVIVPTRELTVQVAKTFEYYMSG 231
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1016
Score = 25.4 bits (53), Expect = 9.5
Identities = 11/18 (61%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +3
Query: 234 NSPYGTKYFD-EYAEEVP 284
NSP TKYFD E+ E+P
Sbjct: 964 NSPTDTKYFDEEFTRELP 981
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 25.4 bits (53), Expect = 9.5
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = -1
Query: 473 YLVRNVYVEEMHLPVLR*EFPSIIPNSASVVQFFLVPLRDGTSN 342
+L N +E+ +LP + F S++P+ +++ + LR SN
Sbjct: 354 FLSTNAVIEKGYLPFVSVAFLSLVPSVMFTMRYLALILRVQNSN 397
>SPAC25B8.03 |||phosphatidylserine decarboxylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 516
Score = 25.4 bits (53), Expect = 9.5
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = +1
Query: 505 AIRSHRSTFWDQRLASAYVTI*GSLRWH 588
+I H T WD + A VT SL WH
Sbjct: 322 SILEHEPTNWDDWVQEADVTDIDSLPWH 349
>SPBC30D10.14 |||dienelactone hydrolase family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 25.4 bits (53), Expect = 9.5
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 1/55 (1%)
Frame = +1
Query: 535 DQRLASAYVTI*GSLRWHI*WPRKVVLSSSIRGRST*RPVPGI-GSCWGGRVLPT 696
DQ+L + + + S H WP+ + ++R G G CWG +VL T
Sbjct: 95 DQKLCNDFFSTRISPNLH--WPKLAKVVEAVRANHGPNVTIGTYGFCWGAKVLVT 147
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,301,544
Number of Sequences: 5004
Number of extensions: 69713
Number of successful extensions: 177
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 168
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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