Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_pT_O21
(574 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 295 4e-81
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 291 4e-80
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 117 1e-27
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 61 1e-10
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 31 0.16
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 0.84
SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 28 0.84
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.6
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 26 3.4
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 4.5
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 6.0
SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces... 25 7.9
SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|... 25 7.9
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 295 bits (723), Expect = 4e-81
Identities = 130/165 (78%), Positives = 147/165 (89%)
Frame = -1
Query: 574 SAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIK 395
SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ +IK
Sbjct: 281 SAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGDVIPRDVQAAVTSIK 340
Query: 394 TKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFD 215
++RTIQFVDWCPTGFK+GI Y+PP VPG +AKV RAVCMLSNTT+IAEAW+RLDHKFD
Sbjct: 341 SRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFD 400
Query: 214 LMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDSAE 80
LMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS +
Sbjct: 401 LMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSMD 445
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 291 bits (715), Expect = 4e-80
Identities = 130/165 (78%), Positives = 145/165 (87%)
Frame = -1
Query: 574 SAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIK 395
SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+LYRGDV+P+DV AA+ TIK
Sbjct: 277 SAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGDVIPRDVQAAVTTIK 336
Query: 394 TKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFD 215
KRTIQFVDWCPTGFK+GI +PP + G ++AKV RAVCMLSNTT+IAEAW+RLDHKFD
Sbjct: 337 AKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFD 396
Query: 214 LMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGMDSAE 80
LMY+KRAFVHWYVGEGMEEGEFSEAREDLAALE+DYEEVG DS E
Sbjct: 397 LMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQDSME 441
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 117 bits (282), Expect = 1e-27
Identities = 59/161 (36%), Positives = 92/161 (57%), Gaps = 3/161 (1%)
Frame = -1
Query: 553 EQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQF 374
+ +SV E+T F+ N MV DPRHG+Y+ L+RG V K+V+ I +++TK + F
Sbjct: 282 QAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYF 341
Query: 373 VDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRA 194
V+W P + PP DL + + + N+T+I E + RL +F M+ ++A
Sbjct: 342 VEWIPDNVLKAVCSVPPK-----DL---KMSATFIGNSTSIQEIFRRLGDQFSAMFRRKA 393
Query: 193 FVHWYVGEGMEEGEFSEAR---EDLAALEKDYEEVGMDSAE 80
F+HWY GEGM+E EF+EA DL + + Y+E G+D +
Sbjct: 394 FLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQEAGIDEGD 434
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 61.3 bits (142), Expect = 1e-10
Identities = 38/151 (25%), Positives = 76/151 (50%), Gaps = 4/151 (2%)
Frame = -1
Query: 544 SVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVD 368
+V ++ P NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+
Sbjct: 291 TVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIP 350
Query: 367 WCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFV 188
W P +V ++ + P + ++ + ML+N T+IA + R ++D + + AF+
Sbjct: 351 WGPASIQVALSKKSPYIKTNHRVSGL-----MLANHTSIASLFKRTLDQYDRLRKRNAFL 405
Query: 187 HWYVGEGMEE---GEFSEAREDLAALEKDYE 104
Y E + E EF +R+ +A L +YE
Sbjct: 406 EQYKKEAIFEDDLNEFDSSRDVVADLINEYE 436
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 30.7 bits (66), Expect = 0.16
Identities = 16/45 (35%), Positives = 21/45 (46%)
Frame = -2
Query: 444 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 310
VV P + RP++P P LS V PV+ V + PP P
Sbjct: 552 VVPEAPSVPQRPAVPVVPEALSVPQPPVAPVAPEVPSVPQPPVAP 596
Score = 25.0 bits (52), Expect = 7.9
Identities = 14/45 (31%), Positives = 18/45 (40%)
Frame = -2
Query: 444 VVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 310
VV P + RP++P P S PV V + PP P
Sbjct: 642 VVPEVPSVPQRPAVPVVPEAPSVPQPPAAPVVPEVPSVPQPPAVP 686
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 28.3 bits (60), Expect = 0.84
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = -1
Query: 532 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 407
+ ACFEP N C H K C L + KD N ++
Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401
>SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 3227
Score = 28.3 bits (60), Expect = 0.84
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = +2
Query: 404 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 547
S G +LGY ++ A++V+A + V HL+ G D + K
Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.6 bits (56), Expect = 2.6
Identities = 20/76 (26%), Positives = 32/76 (42%)
Frame = -2
Query: 549 SFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSS 370
S +P + T+ ++ + +TPV ++ CT TS P T + S P +N +
Sbjct: 417 SSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPY--TSTPVTSTPLTTTNCT 474
Query: 369 TGVQPVSRSVSTTSHP 322
T S TS P
Sbjct: 475 TSTSIPYTSTPVTSTP 490
Score = 26.6 bits (56), Expect = 2.6
Identities = 19/71 (26%), Positives = 34/71 (47%)
Frame = -2
Query: 540 SPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGV 361
+P + T+ ++ + +TPV ++ CT TS P T P S ++ S++
Sbjct: 525 TPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTS 583
Query: 360 QPVSRSVSTTS 328
PV+ + TTS
Sbjct: 584 TPVTTTNCTTS 594
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 26.2 bits (55), Expect = 3.4
Identities = 14/32 (43%), Positives = 21/32 (65%), Gaps = 3/32 (9%)
Frame = -2
Query: 408 SLPSKPSV---LSNSSTGVQPVSRSVSTTSHP 322
+LP KPS+ +++S V+P S STTS+P
Sbjct: 5 TLPPKPSISPSIASSFPTVKPFSSQNSTTSNP 36
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 25.8 bits (54), Expect = 4.5
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = +1
Query: 427 WVRRHHGTAYSKPCTCHDGG 486
W R H Y+KPC DGG
Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 25.4 bits (53), Expect = 6.0
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = -2
Query: 411 PSLPSKPSVLSNSST--GVQPVSRSVSTTSHPPW 316
P PS+P+++SN ST G+Q V V + W
Sbjct: 531 PISPSRPALISNISTKKGIQVVGNMVYDPTRLRW 564
>SPBC3B8.10c |||NLI interacting factor family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 476
Score = 25.0 bits (52), Expect = 7.9
Identities = 15/37 (40%), Positives = 16/37 (43%)
Frame = -2
Query: 429 PRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPP 319
PR LP K VL T + VSR TTS P
Sbjct: 293 PRPLLNSKLPRKTLVLDLDETLIHSVSRGSRTTSGQP 329
>SPAC13G6.10c |||O-glucosyl hydrolase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 530
Score = 25.0 bits (52), Expect = 7.9
Identities = 19/60 (31%), Positives = 26/60 (43%)
Frame = -2
Query: 489 ATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVSRSVSTTSHPPWCP 310
+TP ++S A VV+S T + S S ++SS P S SVS W P
Sbjct: 245 STPAVSSA-AASSSAVVSSVVSSATSVAASSTISSATSSSASASPTSSSVSGKRGLAWIP 303
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,418,069
Number of Sequences: 5004
Number of extensions: 49218
Number of successful extensions: 194
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 244081442
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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