Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_pT_N22
(597 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 212 3e-56
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 212 3e-56
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 212 3e-56
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 77 2e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 40 2e-04
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 4.8
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 6.3
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 8.4
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 8.4
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 212 bits (518), Expect = 3e-56
Identities = 96/127 (75%), Positives = 111/127 (87%)
Frame = -3
Query: 595 VSVKELRRGYVXGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 416
VSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 313 VSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Query: 415 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 236
+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 373 LIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432
Query: 235 VGVIKAV 215
VGVIKAV
Sbjct: 433 VGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 212 bits (518), Expect = 3e-56
Identities = 96/127 (75%), Positives = 111/127 (87%)
Frame = -3
Query: 595 VSVKELRRGYVXGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 416
VSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 313 VSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Query: 415 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 236
+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 373 LIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432
Query: 235 VGVIKAV 215
VGVIKAV
Sbjct: 433 VGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 212 bits (518), Expect = 3e-56
Identities = 96/127 (75%), Positives = 111/127 (87%)
Frame = -3
Query: 595 VSVKELRRGYVXGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 416
VSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE
Sbjct: 313 VSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAE 372
Query: 415 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 236
+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVA
Sbjct: 373 LIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVA 432
Query: 235 VGVIKAV 215
VGVIKAV
Sbjct: 433 VGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 77.0 bits (181), Expect = 2e-15
Identities = 42/125 (33%), Positives = 69/125 (55%), Gaps = 1/125 (0%)
Frame = -3
Query: 583 ELRRGYVXGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 404
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 403 VDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-V 227
+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V
Sbjct: 599 LDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKV 657
Query: 226 IKAVN 212
+K ++
Sbjct: 658 VKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 40.3 bits (90), Expect = 2e-04
Identities = 36/125 (28%), Positives = 56/125 (44%)
Frame = -3
Query: 595 VSVKELRRGYVXGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAE 416
+ V +LR G + + +N P + F A++ + G I +G T VL H+
Sbjct: 474 IEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTS 527
Query: 415 IKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVA 236
+ K+ K + + S K I L PLC+ +E P LGRF +R TVA
Sbjct: 528 VSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVA 584
Query: 235 VGVIK 221
G++K
Sbjct: 585 AGIVK 589
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 4.8
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +3
Query: 171 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 344
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 345 SPDLMDFGLTSVDLPVRRSTFSLIS 419
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 6.3
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +3
Query: 336 MAASPDLMDFGLTSVDLPVRRSTFS 410
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 8.4
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +3
Query: 156 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 266
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 8.4
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +3
Query: 183 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 305
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,371,590
Number of Sequences: 5004
Number of extensions: 45692
Number of successful extensions: 129
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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