Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P12_pT_K20
         (683 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    66   6e-12
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    65   1e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    50   2e-07
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    29   0.47 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   1.4  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   5.8  
SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|...    25   7.7  

>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 65.7 bits (153), Expect = 6e-12
 Identities = 42/134 (31%), Positives = 68/134 (50%)
 Frame = -1

Query: 683 SXYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXEFLPIYSQAKK 504
           S +D  G G+I   ++GDLLRA   NPTLA I                  FL + ++   
Sbjct: 13  SLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQ---FLQVLNRPNG 69

Query: 503 DKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDD 324
               G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K        D
Sbjct: 70  FDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKD 126

Query: 323 GMIPYAAFLKKVMA 282
           GM+ Y  F++ ++A
Sbjct: 127 GMVNYHDFVQMILA 140


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 64.9 bits (151), Expect = 1e-11
 Identities = 41/133 (30%), Positives = 66/133 (49%), Gaps = 1/133 (0%)
 Frame = -1

Query: 683 SXYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXE-FLPIYSQAK 507
           S +D +  G I +  LG ++R+L  +PT A +                   FL + ++  
Sbjct: 19  SLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKM 78

Query: 506 KDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 327
           KD D    E+  E  K++DK+ NG +   ELTH L +LGE+L   EVA++ ++     D 
Sbjct: 79  KDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DTDG 134

Query: 326 DGMIPYAAFLKKV 288
           DG+I Y  F + +
Sbjct: 135 DGVINYEEFSRVI 147



 Score = 29.1 bits (62), Expect = 0.63
 Identities = 15/61 (24%), Positives = 32/61 (52%)
 Frame = -1

Query: 479 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 300
           +F E   L+D++++G +   EL   + +LG+    +E+ ++  +     D +G I +  F
Sbjct: 13  EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70

Query: 299 L 297
           L
Sbjct: 71  L 71


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 33/132 (25%), Positives = 65/132 (49%)
 Frame = -1

Query: 677 YDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXEFLPIYSQAKKDK 498
           YD +  G I   ++G +LR+L  N T A +                  F+     + K +
Sbjct: 18  YDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFV-----SNKLR 72

Query: 497 DQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGM 318
           +  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP  + G 
Sbjct: 73  ETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TNSGS 130

Query: 317 IPYAAFLKKVMA 282
             Y  F++++MA
Sbjct: 131 FDYYDFVQRIMA 142



 Score = 30.7 bits (66), Expect = 0.20
 Identities = 14/60 (23%), Positives = 34/60 (56%)
 Frame = -1

Query: 482 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 303
           ++  E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 29.5 bits (63), Expect = 0.47
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = -1

Query: 521 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 348
           Y+  + +  +   +D  E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 24  YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81



 Score = 27.1 bits (57), Expect = 2.5
 Identities = 18/75 (24%), Positives = 33/75 (44%)
 Frame = -1

Query: 509 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 330
           +K  ++   E+     +L+D +E G +    L      L E +DD E+  + ++     D
Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLD 158

Query: 329 DDGMIPYAAFLKKVM 285
            DG I    F+  +M
Sbjct: 159 QDGEINEQEFIAIMM 173


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 13/31 (41%), Positives = 15/31 (48%)
 Frame = +1

Query: 133  APPEELSPPRALPAPVPQSRASVF*GPSHRT 225
            APP    PP A P P+P S A     P  R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = -1

Query: 395 LGEKLDDSEVAEVTKDCMDPED 330
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


>SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 182

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 12/29 (41%), Positives = 13/29 (44%)
 Frame = +3

Query: 21  ESCTKQLILCCSYKKGVCPYLRCYTGCRR 107
           ESC K+   CC  KK  C       GC R
Sbjct: 78  ESCEKKKPKCCEKKKPKCCESEQNNGCGR 106


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,456,317
Number of Sequences: 5004
Number of extensions: 43751
Number of successful extensions: 135
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)