BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_pT_H08
(698 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 69 6e-13
SPBC947.12 |kms2||spindle pole body protein Kms2|Schizosaccharom... 30 0.28
SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr 2|||M... 28 1.5
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 27 3.4
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 26 4.5
SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 25 7.9
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 25 7.9
>SPAC57A7.04c |pabp||mRNA export shuttling protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 653
Score = 68.9 bits (161), Expect = 6e-13
Identities = 35/69 (50%), Positives = 47/69 (68%)
Frame = -3
Query: 696 TXTMLAAAPLQEQKQMLGERLFPLIQRMHPDLAGKITGMLLEIDNSELLHMLEHAESLKA 517
T LAA P + +KQ+LGE L+P + L+GKITGMLLE+ NSELL +LE +L
Sbjct: 573 TAADLAAVPEESRKQVLGELLYPKVFVREEKLSGKITGMLLEMPNSELLELLEDDSALNE 632
Query: 516 KVDEAVAVL 490
+V+EA+ VL
Sbjct: 633 RVNEAIGVL 641
>SPBC947.12 |kms2||spindle pole body protein
Kms2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 457
Score = 30.3 bits (65), Expect = 0.28
Identities = 14/43 (32%), Positives = 21/43 (48%)
Frame = +3
Query: 495 QQQLHLL*LSETLHVLTYEEVLNYQFPKAYQLFSQPNQGAFSE 623
QQQ H++ + E +H + E + K L Q +GAF E
Sbjct: 249 QQQAHIVRMEELIHAVEKERKTETDYMKKESLSEQKERGAFME 291
>SPBC29A3.06 |||CGI-48 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 556
Score = 27.9 bits (59), Expect = 1.5
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 1/60 (1%)
Frame = -3
Query: 684 LAAAPLQEQKQMLGERLF-PLIQRMHPDLAGKITGMLLEIDNSELLHMLEHAESLKAKVD 508
L +A +Q+ +M G+ F P ++R H D GK + + LLH L + K++
Sbjct: 290 LESAQVQKVSRMYGQENFQPSMERFHVDPTGKYIALEGRSGHINLLHALTGQFATSFKIE 349
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 26.6 bits (56), Expect = 3.4
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = -2
Query: 160 ENFRYIHFSLIYYLILWELALVLSF 86
+NF Y+ F ++ L++W++ + SF
Sbjct: 184 KNFAYLTFISLFVLVIWQILYLRSF 208
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
Frame = -3
Query: 624 IQRMHPDLAGKITGMLLEID-NSELLHMLEHAESLKAKVDEAV 499
+QR++P ++ G LL+ID + EL+ L + + VDE V
Sbjct: 801 VQRINPSKTPQVVGALLDIDCDEELVQNLLMSVVGQVPVDELV 843
>SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1888
Score = 25.4 bits (53), Expect = 7.9
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = -3
Query: 297 YALLCNFKECKFTNKNT*LSTFLRCELMNSLTFTLTKKFIL 175
Y L+ +F TNK + L + L C L N L + + F+L
Sbjct: 1718 YYLISSFVAISMTNKTSGLPSSLICNLQNFLA-RIARDFLL 1757
>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 881
Score = 25.4 bits (53), Expect = 7.9
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = -2
Query: 157 NFRYIHFSLIYYLILWELALVLSFIITFTSVF 62
N I ++L+YYLI+ L+ S I FT F
Sbjct: 317 NASSIIYTLMYYLIIAPTLLITSAICMFTIFF 348
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,361,595
Number of Sequences: 5004
Number of extensions: 40307
Number of successful extensions: 123
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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