Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P12_pT_B18
         (726 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa...    73   6e-14
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    47   3e-06
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    38   0.001
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    30   0.39 
SPBC106.07c |||N alpha-acetyltransferase Nat2 |Schizosaccharomyc...    27   2.1  
SPCC18.13 |||tRNA |Schizosaccharomyces pombe|chr 3|||Manual            26   6.3  
SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces ...    26   6.3  

>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 210

 Score = 72.5 bits (170), Expect = 6e-14
 Identities = 38/118 (32%), Positives = 66/118 (55%), Gaps = 3/118 (2%)
 Frame = -3

Query: 703 FTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGVG-DHATVLTQ--LETSAEEI 533
           FT    G+Y+ CF N  STF+ K+V ME+ +  E  LP +  D A    +  ++++  EI
Sbjct: 85  FTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDEAKDYKKDSMQSTVLEI 144

Query: 532 HSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNFF 359
            +AL+ I   Q + + RE +     +    ++FW+S+ E++ +V ++  QV I+K FF
Sbjct: 145 STALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVVALSALQVFIVKTFF 202


>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 46.8 bits (106), Expect = 3e-06
 Identities = 31/121 (25%), Positives = 51/121 (42%), Gaps = 1/121 (0%)
 Frame = -3

Query: 709 HQFTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGVGDH-ATVLTQLETSAEEI 533
           + FT    G Y  CF N+      K V   ++      +  + D        L     ++
Sbjct: 81  YSFTVKNPGKYMYCFYNDALDGESKEVLFNVH-----GVIYISDEDLDANNPLLGKVRQL 135

Query: 532 HSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNFFSD 353
           H  ++++   Q +   RE   R  AE  N++V WWS+ +T+ +V V   Q+  LK  F  
Sbjct: 136 HDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLKRLFEV 195

Query: 352 K 350
           K
Sbjct: 196 K 196


>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 37.9 bits (84), Expect = 0.001
 Identities = 22/118 (18%), Positives = 51/118 (43%), Gaps = 2/118 (1%)
 Frame = -3

Query: 706 QFTALASGDYKVCF--SNEFSTFSHKLVYMELNVGPEQPLPGVGDHATVLTQLETSAEEI 533
           +FT    G +++C+  +++ + FS     + +++  +     +         L      +
Sbjct: 92  RFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIASSEDEAKDLTDRVAAL 151

Query: 532 HSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNFF 359
           ++ L  I+  Q   R RE   R  +E  N +V  W++ + + ++     Q+  L+ FF
Sbjct: 152 NTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLLVTCIWQLSHLQRFF 209


>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 29.9 bits (64), Expect = 0.39
 Identities = 28/124 (22%), Positives = 48/124 (38%), Gaps = 6/124 (4%)
 Frame = -3

Query: 703 FTALASGDYKVCFSNEF------STFSHKLVYMELNVGPEQPLPGVGDHATVLTQLETSA 542
           F   AS    +CF N        S    + V +E  VG +         A  L  +E   
Sbjct: 89  FDVDASAMLDICFLNTLTPGAIESEHKKRSVKLEFTVGADADDYSSLQKANNLEPVEADI 148

Query: 541 EEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNF 362
                 +  I     + + REA+ R   E  NE+V  ++    +++  +   Q++ L++F
Sbjct: 149 RRARDFIEEIKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVLVIWQILYLRSF 208

Query: 361 FSDK 350
           F  K
Sbjct: 209 FQRK 212


>SPBC106.07c |||N alpha-acetyltransferase Nat2 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 167

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 10/32 (31%), Positives = 19/32 (59%)
 Frame = -3

Query: 466 KRAEDLNEKVFWWSMGETLAIVCVAFTQVMIL 371
           +R ++L +K  WWS+G  + I  + F+   +L
Sbjct: 52  QRVKELTKKYGWWSLGVYIGISVLDFSASFVL 83


>SPCC18.13 |||tRNA |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 421

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = -1

Query: 306 NLLKFFCLFEFDLLNSFIKYFLINCVAIVSI 214
           +L  F CL  FDL ++F  Y  +N   +VS+
Sbjct: 253 DLENFKCLDAFDLRSAFSTYLSLNQPMVVSV 283


>SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 318

 Score = 25.8 bits (54), Expect = 6.3
 Identities = 10/23 (43%), Positives = 14/23 (60%)
 Frame = -2

Query: 431 VVYG*NLGYCLCCIYTSYDTKEF 363
           +V   N+G C CC +  Y TK+F
Sbjct: 65  IVSNHNVG-CSCCYFRQYSTKQF 86


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,681,457
Number of Sequences: 5004
Number of extensions: 49407
Number of successful extensions: 148
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)