Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_L06
(784 letters)
Database: mosquito
2352 sequences; 563,979 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha ... 33 0.008
AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ... 32 0.017
AF492464-1|AAM11657.1| 803|Anopheles gambiae beta nu integrin s... 26 1.5
EF990672-1|ABS30733.1| 466|Anopheles gambiae voltage-gated calc... 23 8.1
AY578798-1|AAT07303.1| 356|Anopheles gambiae baboon protein. 23 8.1
>AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha 1
chain precursor protein.
Length = 801
Score = 33.5 bits (73), Expect = 0.008
Identities = 22/61 (36%), Positives = 28/61 (45%), Gaps = 3/61 (4%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG--ICECKP-TDNSENERGDSASRPSKTTCSNVPASDPKPQWMGE 300
GL G KG+RG G G +C+P + ERG A P + S VP P GE
Sbjct: 562 GLPGAKGERGLKGELGGRCTDCRPGMKGDKGERG-YAGEPGRPGASGVPGERGYPGMPGE 620
Query: 301 N 303
+
Sbjct: 621 D 621
Score = 32.3 bits (70), Expect = 0.017
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G KGEKGDRG PG PG
Sbjct: 302 GPKGEKGDRGEPGEPG 317
Score = 31.5 bits (68), Expect = 0.030
Identities = 15/32 (46%), Positives = 17/32 (53%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENERGD 225
G +GE G RG+PG PG T ERGD
Sbjct: 241 GPQGEVGPRGFPGRPGEKGVPGTPGVRGERGD 272
Score = 30.7 bits (66), Expect = 0.053
Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
Frame = +1
Query: 118 KIDAGLKGEKGDRGY---PGPPGICECKPTDNSENERGDSASRPSK 246
K + G G+KGD+GY G PG C P + E RG + K
Sbjct: 737 KGNVGYSGDKGDKGYSGLKGEPGRCASIPPNLEEAIRGPQGLQGEK 782
Score = 29.5 bits (63), Expect = 0.12
Identities = 14/31 (45%), Positives = 15/31 (48%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENERG 222
G G KG RGY GP G D + ERG
Sbjct: 411 GAPGPKGPRGYEGPQGPKGMDGFDGEKGERG 441
Score = 29.1 bits (62), Expect = 0.16
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
GL GEKG +G PGP G+
Sbjct: 127 GLPGEKGTKGEPGPVGL 143
Score = 28.7 bits (61), Expect = 0.21
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
GLKG KG RG+PG G+
Sbjct: 112 GLKGAKGVRGFPGSEGL 128
Score = 28.7 bits (61), Expect = 0.21
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G+ G +G RGYPG PG
Sbjct: 474 GMPGPQGPRGYPGQPG 489
Score = 28.7 bits (61), Expect = 0.21
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +1
Query: 118 KIDAGLKGEKGDRGYPGPPG 177
K + GL G KG++G+PGP G
Sbjct: 669 KGENGLMGIKGEKGFPGPVG 688
Score = 28.3 bits (60), Expect = 0.28
Identities = 20/61 (32%), Positives = 26/61 (42%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENERGDSASRPSKTTCSNVPASDPKPQWMGENEL 309
GLKG+KG+RG+ G G P D E G + P + P P GE L
Sbjct: 518 GLKGQKGERGFKGVMG----TPGDAKEGRPG-APGLPGRDGEKGEPGRPGLPGAKGERGL 572
Query: 310 E 312
+
Sbjct: 573 K 573
Score = 28.3 bits (60), Expect = 0.28
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
GL+G KGD+G PG GI
Sbjct: 694 GLRGMKGDKGRPGEAGI 710
Score = 27.1 bits (57), Expect = 0.65
Identities = 15/47 (31%), Positives = 19/47 (40%)
Frame = +1
Query: 142 EKGDRGYPGPPGICECKPTDNSENERGDSASRPSKTTCSNVPASDPK 282
EKG+RG PGP G+ K G + +K V PK
Sbjct: 101 EKGNRGLPGPMGLKGAKGVRGFPGSEGLPGEKGTKGEPGPVGLQGPK 147
Score = 27.1 bits (57), Expect = 0.65
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
GL+G KGDRG G PG
Sbjct: 142 GLQGPKGDRGRDGLPG 157
Score = 26.6 bits (56), Expect = 0.87
Identities = 15/49 (30%), Positives = 20/49 (40%), Gaps = 2/49 (4%)
Frame = +1
Query: 127 AGLKGEKGDRGYPGPPGIC--ECKPTDNSENERGDSASRPSKTTCSNVP 267
+GL G G RGY G PG + +P + EN P +P
Sbjct: 192 SGLPGNPGPRGYAGIPGTKGEKGEPARHPENYNKGQKGEPGNDGLEGLP 240
Score = 26.6 bits (56), Expect = 0.87
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G +G KG++G PG PG
Sbjct: 332 GERGHKGEKGLPGQPG 347
Score = 26.6 bits (56), Expect = 0.87
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +1
Query: 118 KIDAGLKGEKGDRGYPGPPGI 180
K +G KGE G G PG PGI
Sbjct: 376 KGQSGPKGEPGRDGIPGQPGI 396
Score = 26.6 bits (56), Expect = 0.87
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPG 177
D G KGE G G PGP G
Sbjct: 463 DKGDKGESGSVGMPGPQG 480
Score = 26.2 bits (55), Expect = 1.1
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G GEKG+RG GP G
Sbjct: 432 GFDGEKGERGQMGPKG 447
Score = 25.8 bits (54), Expect = 1.5
Identities = 11/18 (61%), Positives = 12/18 (66%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPG 177
+ G KGEKG G PGP G
Sbjct: 333 ERGHKGEKGLPGQPGPRG 350
Score = 25.0 bits (52), Expect = 2.6
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
G KG++G G PG PGI
Sbjct: 145 GPKGDRGRDGLPGYPGI 161
Score = 24.6 bits (51), Expect = 3.5
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
GL G+KGDRG G G+
Sbjct: 359 GLPGQKGDRGSEGLHGL 375
Score = 23.8 bits (49), Expect = 6.1
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G+KGEKG G GP G
Sbjct: 676 GIKGEKGFPGPVGPEG 691
Score = 23.4 bits (48), Expect = 8.1
Identities = 15/41 (36%), Positives = 19/41 (46%), Gaps = 3/41 (7%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGP---PGICECKPTDNSENERGDSASR 237
+ G KGE G G PGP PG P D + +GD +
Sbjct: 629 EPGPKGEPGLLGPPGPSGEPGRDAEIPMDQLKPIKGDKGEK 669
>AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1
chain protein.
Length = 1024
Score = 32.3 bits (70), Expect = 0.017
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
GL G KGDRG PG PG+
Sbjct: 451 GLSGRKGDRGVPGSPGL 467
Score = 31.5 bits (68), Expect = 0.030
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G+ GEKGD+GY GP G
Sbjct: 287 GMSGEKGDKGYTGPEG 302
Score = 31.1 bits (67), Expect = 0.040
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G GEKGD G PGPPG
Sbjct: 137 GYPGEKGDLGTPGPPG 152
Score = 29.1 bits (62), Expect = 0.16
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
G+ GEKGDRG PG G+
Sbjct: 678 GMVGEKGDRGLPGMSGL 694
Score = 28.3 bits (60), Expect = 0.28
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
GL G KGD G PG PG+
Sbjct: 370 GLNGVKGDMGVPGFPGV 386
Score = 28.3 bits (60), Expect = 0.28
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPGI 180
+ GL GEKGD G GP G+
Sbjct: 434 ERGLMGEKGDMGLTGPVGL 452
Score = 27.5 bits (58), Expect = 0.50
Identities = 13/36 (36%), Positives = 18/36 (50%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENERGDSASR 237
GLKG +G +G G PGI + + E G+ R
Sbjct: 215 GLKGFQGRKGMMGAPGIQGVRGPQGVKGEPGEKGDR 250
Score = 27.5 bits (58), Expect = 0.50
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPG 177
+AG KGE G +G PG PG
Sbjct: 501 EAGAKGEMGIQGLPGLPG 518
Score = 27.1 bits (57), Expect = 0.65
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G+KG+KG G PG PG
Sbjct: 385 GVKGDKGTTGLPGIPG 400
Score = 27.1 bits (57), Expect = 0.65
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENER 219
GL G +G++G GPPG K D E +R
Sbjct: 621 GLPGPQGEKGDQGPPGFIGPK-GDKGERDR 649
Score = 27.1 bits (57), Expect = 0.65
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENERG 222
G +G KGDRG PG G+ + +RG
Sbjct: 657 GPQGMKGDRGMPGLEGVAGLPGMVGEKGDRG 687
Score = 27.1 bits (57), Expect = 0.65
Identities = 13/26 (50%), Positives = 13/26 (50%)
Frame = +1
Query: 100 PDFISCKIDAGLKGEKGDRGYPGPPG 177
P F K D GL G G G PG PG
Sbjct: 721 PGFNGPKGDKGLPGLAGPAGIPGAPG 746
Score = 26.6 bits (56), Expect = 0.87
Identities = 15/32 (46%), Positives = 17/32 (53%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGICECKPTDNSENERGD 225
GLKG+KG G PGP C P E+GD
Sbjct: 269 GLKGDKGLAGLPGP----SCLP--GMSGEKGD 294
Score = 26.6 bits (56), Expect = 0.87
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPG 177
D+GL G G+ G PGP G
Sbjct: 601 DSGLMGRPGNDGLPGPQG 618
Score = 26.6 bits (56), Expect = 0.87
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
GL G +G RG PGP G
Sbjct: 612 GLPGPQGQRGLPGPQG 627
Score = 25.8 bits (54), Expect = 1.5
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G +GEKG+ G GPPG
Sbjct: 60 GHRGEKGNSGPVGPPG 75
Score = 25.8 bits (54), Expect = 1.5
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = +1
Query: 127 AGLKGEKGDRGYPGPPG 177
A +KG+KG+ G+PG G
Sbjct: 472 AAIKGDKGEPGFPGAIG 488
Score = 25.8 bits (54), Expect = 1.5
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = +1
Query: 127 AGLKGEKGDRGYPGPPGICECKPTDNSENERGDSASRP 240
AGL G G++G G PG+ + ++G++ P
Sbjct: 674 AGLPGMVGEKGDRGLPGMSGLNGAPGEKGQKGETPQLP 711
Score = 25.0 bits (52), Expect = 2.6
Identities = 12/21 (57%), Positives = 13/21 (61%), Gaps = 3/21 (14%)
Frame = +1
Query: 124 DAGLKGE---KGDRGYPGPPG 177
D G KGE KG G+PG PG
Sbjct: 156 DVGPKGEPGPKGPAGHPGAPG 176
Score = 25.0 bits (52), Expect = 2.6
Identities = 15/38 (39%), Positives = 18/38 (47%), Gaps = 5/38 (13%)
Frame = +1
Query: 130 GLKGEKGDRGYP-----GPPGICECKPTDNSENERGDS 228
GL GEKG+ G P GP G + +RGDS
Sbjct: 565 GLTGEKGEPGLPVWKDRGPSGPSGPLGPQGEKGDRGDS 602
Score = 24.6 bits (51), Expect = 3.5
Identities = 9/19 (47%), Positives = 11/19 (57%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPGI 180
+ G EKG G PG PG+
Sbjct: 306 EPGAASEKGQNGEPGVPGL 324
Score = 24.6 bits (51), Expect = 3.5
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPGI 180
G G KGD+G G PGI
Sbjct: 382 GFPGVKGDKGTTGLPGI 398
Score = 24.6 bits (51), Expect = 3.5
Identities = 13/40 (32%), Positives = 17/40 (42%)
Frame = +1
Query: 127 AGLKGEKGDRGYPGPPGICECKPTDNSENERGDSASRPSK 246
+G KG++G G PG P D E + RP K
Sbjct: 453 SGRKGDRGVPGSPGLPATVAAIKGDKGEPGFPGAIGRPGK 492
Score = 24.6 bits (51), Expect = 3.5
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = +1
Query: 118 KIDAGLKGEKGDRGYPGPPGI 180
K + GL G G G GPPG+
Sbjct: 546 KGEKGLPGRPGKTGRDGPPGL 566
Score = 24.2 bits (50), Expect = 4.6
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +1
Query: 124 DAGLKGEKGDRGYPGPPGICECKPTDNSENERGDS 228
+ G G +G+ G PG PG + E+G+S
Sbjct: 34 EQGRTGAQGNAGPPGAPGPVGPRGLTGHRGEKGNS 68
Score = 23.8 bits (49), Expect = 6.1
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 130 GLKGEKGDRGYPGPPG 177
G KG++GD G G PG
Sbjct: 594 GEKGDRGDSGLMGRPG 609
Score = 23.4 bits (48), Expect = 8.1
Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Frame = +1
Query: 118 KIDAGLKGEKGDRGYPGPPGICECKPTDNSENERGDSASRPSKTTCSNV-PASDPKPQ 288
K + GL+G KG+RG G G + E+GD + +V P +P P+
Sbjct: 109 KGNPGLRGPKGERGGMGDRGDPGLPGSLGYPGEKGDLGTPGPPGYPGDVGPKGEPGPK 166
Score = 23.4 bits (48), Expect = 8.1
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = +1
Query: 118 KIDAGLKGEKGDRGYPGPPGICECK 192
K D G G KG G GPPG+ K
Sbjct: 247 KGDRGEIGVKGLMGQSGPPGMIGLK 271
Score = 23.4 bits (48), Expect = 8.1
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +1
Query: 91 QXGPDFISCKIDAGLKGEKGDRGYPGPPGI 180
Q P FI K D G + G G GP G+
Sbjct: 632 QGPPGFIGPKGDKGERDRDGLNGLNGPQGM 661
>AF492464-1|AAM11657.1| 803|Anopheles gambiae beta nu integrin
subunit AgBnu protein.
Length = 803
Score = 25.8 bits (54), Expect = 1.5
Identities = 16/59 (27%), Positives = 25/59 (42%), Gaps = 1/59 (1%)
Frame = +1
Query: 166 GPP-GICECKPTDNSENERGDSASRPSKTTCSNVPASDPKPQWMGENELESFKIECDEA 339
GP GIC C ++ GD+ + TT P++D G + + CDE+
Sbjct: 572 GPDHGICTCGTCSCFDSWSGDNCECTTDTTGCKAPSND--AVCSGHGQCNCGRCSCDES 628
>EF990672-1|ABS30733.1| 466|Anopheles gambiae voltage-gated calcium
channel beta subunitprotein.
Length = 466
Score = 23.4 bits (48), Expect = 8.1
Identities = 16/60 (26%), Positives = 24/60 (40%), Gaps = 1/60 (1%)
Frame = +1
Query: 139 GEKGDRGYPG-PPGICECKPTDNSENERGDSASRPSKTTCSNVPASDPKPQWMGENELES 315
G G G PG P PT +++ ASR KT + P + + + + E S
Sbjct: 174 GNLGASGVPGAEPSRGSTPPTPGDDSD-SMGASRHGKTPLATPPTKEKRKPFFKKQETSS 232
>AY578798-1|AAT07303.1| 356|Anopheles gambiae baboon protein.
Length = 356
Score = 23.4 bits (48), Expect = 8.1
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +1
Query: 355 IAHETFSQKTILVKKNFT 408
IAH K ILVK N T
Sbjct: 183 IAHRDLKSKNILVKSNLT 200
Database: mosquito
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 563,979
Number of sequences in database: 2352
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 810,667
Number of Sequences: 2352
Number of extensions: 17291
Number of successful extensions: 191
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 178
length of database: 563,979
effective HSP length: 63
effective length of database: 415,803
effective search space used: 81913191
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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