Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_L01
(760 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.06 |gpm1||monomeric 2,3-bisphosphoglycerate |Schizosacc... 177 1e-45
SPAC1687.21 ||SPAC222.01|phosphoglycerate mutase family |Schizos... 53 5e-08
SPCC1620.13 |||phosphoglycerate mutase family|Schizosaccharomyce... 36 0.008
SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity ... 34 0.019
SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|... 29 0.54
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 29 0.72
SPCC645.08c |snd1||RNA-binding protein Snd1|Schizosaccharomyces ... 27 2.9
SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 26 6.7
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 25 8.9
SPAC1002.19 |urg1||GTP cyclohydrolase II |Schizosaccharomyces po... 25 8.9
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 25 8.9
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 25 8.9
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 8.9
>SPAC26F1.06 |gpm1||monomeric 2,3-bisphosphoglycerate
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 211
Score = 177 bits (432), Expect = 1e-45
Identities = 98/223 (43%), Positives = 132/223 (59%)
Frame = +2
Query: 92 IVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAAGKALKAEGYQFDVAHTSVLKRAQI 271
+V+ RHGESEWN+ NLF GW D LS+ G +EA G+ LK+ GY+FD+A TS L+RAQ
Sbjct: 10 LVLTRHGESEWNKLNLFTGWKDPALSETGIKEAKLGGERLKSRGYKFDIAFTSALQRAQK 69
Query: 272 TLNSILKEIGQPDIPIEKTWRLNERHYGGLTGLNKAETAAKYGEAQVQIWRRSFDVPPPA 451
T IL+E+G+P++ K+ +LNER+YG L GLNK + K+G QVQIWRRS+D+ PP
Sbjct: 70 TCQIILEEVGEPNLETIKSEKLNERYYGDLQGLNKDDARKKWGAEQVQIWRRSYDIAPPN 129
Query: 452 MEKDHPYYDTIVNDPRYAADPKPEEFPMYESLKLTIERTLPYWNNVIVPQIKEGKKIIIA 631
E D P Y+S IVP I +G+K++IA
Sbjct: 130 GES--------------LKDTAERVLPYYKS--------------TIVPHILKGEKVLIA 161
Query: 632 AHGNSLRGIVKHLXDLSDAXIMELNLPTGIPFVYELDXNLKPV 760
AHGNSLR ++ L L+ I++ L TG+P VY LD + K V
Sbjct: 162 AHGNSLRALIMDLEGLTGDQIVKRELATGVPIVYHLDKDGKYV 204
>SPAC1687.21 ||SPAC222.01|phosphoglycerate mutase family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 209
Score = 52.8 bits (121), Expect = 5e-08
Identities = 31/98 (31%), Positives = 53/98 (54%)
Frame = +2
Query: 89 KIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEAVAAGKALKAEGYQFDVAHTSVLKRAQ 268
K+ +IRHG+++ N++ + G D +L++ GR +A + L D S +KR +
Sbjct: 2 KVFLIRHGQTDQNKRGILQGSVDTNLNETGRLQAKLLAQRLLP--LDIDQIFCSSMKRCR 59
Query: 269 ITLNSILKEIGQPDIPIEKTWRLNERHYGGLTGLNKAE 382
T+ L+ +P++PI T + ER YG L G+N E
Sbjct: 60 ETIAPYLEL--KPEVPIVYTDLIRERVYGDLEGMNVVE 95
Score = 28.3 bits (60), Expect = 1.3
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +2
Query: 539 ESLKLTIERTLPYWNNVIVPQIKEGKKIIIAAHGNSLRGIVKHLXD 676
E L R L +W+ +VP + K +I+ HG + + H +
Sbjct: 110 EGLSHLTSRLLKFWDEYVVPLQGKKKCVIVLCHGGVINVLRTHFME 155
>SPCC1620.13 |||phosphoglycerate mutase family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 282
Score = 35.5 bits (78), Expect = 0.008
Identities = 29/109 (26%), Positives = 53/109 (48%), Gaps = 1/109 (0%)
Frame = +2
Query: 65 SNKMPAKYKIVMIRHGESEWNQKNLFCG-WFDADLSDKGRQEAVAAGKALKAEGYQFDVA 241
S++ +K+ +++RH ESE N + + G D++L+ G +A K+++
Sbjct: 47 SSQNDSKFTCLLVRHAESEHNVRGIRAGARIDSELTVHGYNQAKKLAKSIR--NLDIVCV 104
Query: 242 HTSVLKRAQITLNSILKEIGQPDIPIEKTWRLNERHYGGLTGLNKAETA 388
++S KRA+ T I K + P+ + L E+ G L G + TA
Sbjct: 105 YSSPQKRAKRTAEEITK---VANCPLYISDFLMEKDLGSLEGTSFRYTA 150
>SPAC732.02c |||fructose-2,6-bisphosphate 2-phosphatase activity
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 408
Score = 34.3 bits (75), Expect = 0.019
Identities = 20/59 (33%), Positives = 33/59 (55%)
Frame = +2
Query: 14 VFHRVLSVICSRSEIYLSNKMPAKYKIVMIRHGESEWNQKNLFCGWFDADLSDKGRQEA 190
+ H++ + + SR YLSN + I + RHGES++N + G D+ LS +G + A
Sbjct: 183 IVHKLENYLESRIVYYLSNLRTRRRSIWLSRHGESQFNVEGKIGG--DSSLSPQGLKYA 239
>SPAC2E1P3.04 |||copper amine oxidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 712
Score = 29.5 bits (63), Expect = 0.54
Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +1
Query: 562 KNPTLLEQCYCASDQRRQEDH-YCCPW 639
K+P ++EQC + Q DH YC PW
Sbjct: 133 KDPAVIEQCILSGVPPDQMDHVYCDPW 159
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 29.1 bits (62), Expect = 0.72
Identities = 20/44 (45%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
Frame = -1
Query: 460 FFHGRW-RNVEATAPDLNLS--LPVFGSCLSLVQSSEATIMPLV 338
FF G W N AT +N+ + VFGSCLS V SS I+P +
Sbjct: 349 FFQGLWFGNHLATTKRVNVGQVVTVFGSCLS-VASSLQQILPAI 391
>SPCC645.08c |snd1||RNA-binding protein Snd1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 878
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
Frame = -1
Query: 451 GRWRNVEATAPDLN-LSLPVFGSCLSLVQSSE 359
G W+N+ + PD+N LSL + + +S V S++
Sbjct: 308 GIWKNISVSIPDINSLSLKDYSAVVSRVISTD 339
>SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 857
Score = 25.8 bits (54), Expect = 6.7
Identities = 12/51 (23%), Positives = 27/51 (52%)
Frame = -2
Query: 753 FKXSSSSYTKGMPVGRFNSMIXASLRSXKCFTIPLRLLPWAAIMIFLPSLI 601
F + Y + + +S + ++ + KCF + +L +AI++FL S++
Sbjct: 660 FSNLETCYESAKAIVQLSSKLLSAGQMDKCFYLEFEILYCSAIVLFLFSVM 710
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 25.4 bits (53), Expect = 8.9
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +2
Query: 506 ADPKPEEFPMYESLKLTIERTLPYWNNVIVPQIKEGK 616
A+P E P Y++L + T Y +P+ K+GK
Sbjct: 98 AEPIEESSPSYQALSSMAKDTKTYLFGGSIPERKDGK 134
>SPAC1002.19 |urg1||GTP cyclohydrolase II |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 439
Score = 25.4 bits (53), Expect = 8.9
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = +2
Query: 218 EGYQFDVAHTSVLKRAQITLNSILKEIGQPDIPIEKTWRLNER 346
EGY DV T + RA + + I + + +PI+ LNE+
Sbjct: 146 EGY--DVRPTIAITRAHLQVTEIQRSVENGSLPIDGKIVLNEK 186
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 545 LKLTIERTLPYWNNVIVPQIKEGKKIIIAAHGN 643
++L + +TLP+ N I+ +E K +I + GN
Sbjct: 1 MQLKLTKTLPFSENFIMADSEEYKTVIGISFGN 33
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.4 bits (53), Expect = 8.9
Identities = 12/48 (25%), Positives = 22/48 (45%)
Frame = +2
Query: 431 FDVPPPAMEKDHPYYDTIVNDPRYAADPKPEEFPMYESLKLTIERTLP 574
FD PP A++ H Y +++ R A P F ++ + ++ P
Sbjct: 466 FDPPPSAVKTSHNYGLPFLSNQRCPATPTRNPFAFENTVSIHMDGRQP 513
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 8.9
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +1
Query: 97 YDSSWRIRMEPEESLL 144
+D SW++R E ESLL
Sbjct: 15 FDKSWKVRFEAYESLL 30
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,987,307
Number of Sequences: 5004
Number of extensions: 59146
Number of successful extensions: 150
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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