Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_K21
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma c... 30 0.38
SPBC1711.10c |npl4||Cdc48-Ufd1-Npl4 complex component Npl4 |Schi... 29 0.87
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 29 0.87
SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|ch... 28 1.2
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 27 3.5
SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizos... 27 3.5
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 26 4.7
SPAC664.09 |ggt1||gamma-glutamyltranspeptidase Ggt1 |Schizosacch... 26 4.7
SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting... 26 6.1
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 26 6.1
SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe... 26 6.1
SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr 1|... 25 8.1
SPAC31A2.10 |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 8.1
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 8.1
>SPCC24B10.22 ||SPCPB16A4.01|mitochondrial DNA polymerase gamma
catalytic subunit|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1018
Score = 29.9 bits (64), Expect = 0.38
Identities = 17/71 (23%), Positives = 34/71 (47%)
Frame = +1
Query: 502 SSELRVNEFNIQMISKNIYEQLFKKPSSSPDPEVIKSCQESLLRHGIXYKKCSNYLPDVT 681
++ LR+N +Q +S + Q+F + ++ + + L +H + K+ LP
Sbjct: 39 NAPLRINPVGVQYLSPALQNQVFPQQNTQISQLHLDLAKFHLAKHQLLNKETIK-LPSFN 97
Query: 682 LKLPKLXGQDI 714
+LP L G+ I
Sbjct: 98 FRLPPLQGKTI 108
>SPBC1711.10c |npl4||Cdc48-Ufd1-Npl4 complex component Npl4
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 545
Score = 28.7 bits (61), Expect = 0.87
Identities = 13/57 (22%), Positives = 26/57 (45%)
Frame = +1
Query: 496 NLSSELRVNEFNIQMISKNIYEQLFKKPSSSPDPEVIKSCQESLLRHGIXYKKCSNY 666
NL+ E+ V + + I +Y+ +PS PD ++K ++ + Y+ Y
Sbjct: 391 NLNGEIEVMSYQVSNIGTALYQADLIQPSVDPDRMLVKKEDQTRYVPDVLYRYTDKY 447
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 28.7 bits (61), Expect = 0.87
Identities = 23/85 (27%), Positives = 46/85 (54%), Gaps = 3/85 (3%)
Frame = +1
Query: 367 SFLNKVNVGNQRFLSDVLPTTEFKVLKKQKKRN-DIPKVNNEEPN--LSSELRVNEFNIQ 537
S +++ + ++ F+ DV EF+++ +KK++ DIP P ++ ELR + NIQ
Sbjct: 251 SRISQTDTWDETFIFDVHRAKEFQIIIYEKKKDFDIPIALILIPTTIIAEELR-RKRNIQ 309
Query: 538 MISKNIYEQLFKKPSSSPDPEVIKS 612
+S+ ++ + +S D + KS
Sbjct: 310 EMSETSWKPSIAESASRSDEKGSKS 334
>SPCP25A2.03 |||THO complex subunit |Schizosaccharomyces pombe|chr
3|||Manual
Length = 752
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/52 (25%), Positives = 28/52 (53%)
Frame = +1
Query: 442 LKKQKKRNDIPKVNNEEPNLSSELRVNEFNIQMISKNIYEQLFKKPSSSPDP 597
+++ K +ND+PKV+ + P + VN ++ ++K + + +S DP
Sbjct: 604 VEEGKHKNDLPKVSPKPPTEGVDSEVNGESLVQVNKVLKSEDDNTSEASKDP 655
>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
Raf2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +1
Query: 460 RNDIPKVNNEEPNLSSELRVNEFNIQMISKNIYEQLFKKPSSSPDPEVIKSC 615
RN PKV + PNL ++ ++ N+ +++ L +P+ D SC
Sbjct: 490 RNKYPKVYSSTPNLCDQVWLDRTNLFPCNRSTRSTLPYRPTKLLDLASASSC 541
>SPCC306.04c |set1||histone lysine methyltransferase
Set1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 920
Score = 26.6 bits (56), Expect = 3.5
Identities = 20/55 (36%), Positives = 26/55 (47%)
Frame = +1
Query: 544 SKNIYEQLFKKPSSSPDPEVIKSCQESLLRHGIXYKKCSNYLPDVTLKLPKLXGQ 708
SK I +FK S P P V + QE+LL S +PDV LK+ G+
Sbjct: 392 SKIIGPAIFKYLHSMPKPSVKEELQENLL-------VSSTSVPDVPLKIESTVGK 439
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 26.2 bits (55), Expect = 4.7
Identities = 16/39 (41%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = +1
Query: 511 LRVNEFNI-QMISKNIYEQLFKKPSSSPDPEVIKSCQES 624
L VNE I Q K + +LF PSS E+ + CQE+
Sbjct: 141 LIVNEKIIPQKTQKYLLYELFSSPSSITANEIARLCQEA 179
>SPAC664.09 |ggt1||gamma-glutamyltranspeptidase Ggt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 630
Score = 26.2 bits (55), Expect = 4.7
Identities = 16/49 (32%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = -1
Query: 189 KSHPSWLNLCSPNGPG-ETGKAFGTPAIQFHY*AINKFTEPLFLYNSQI 46
K+HP W + +P G G+ F PA+ I KF +F Y +I
Sbjct: 246 KTHPDWSKIFAPEGVFLHVGEKFYRPALASTLEEIAKFGPEVF-YTGKI 293
>SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting
complex subunit Apc4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 719
Score = 25.8 bits (54), Expect = 6.1
Identities = 16/58 (27%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
Frame = +1
Query: 499 LSSELRVNEFNIQMISKNIYEQLFKKPSSSPDPEVIKSCQESL--LRHGIXYKKCSNY 666
L E + + I ++ I E +PSS P E+I+ E++ +RH + K ++Y
Sbjct: 419 LFEEKKYMKHFISWLNYAIVEFNTSEPSSIPPQEIIEHINETVIYIRHSLFRSKLTSY 476
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 25.8 bits (54), Expect = 6.1
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +3
Query: 120 YRRPFQFHQDRWASKGLARR 179
Y P F+ DRWA GLA +
Sbjct: 425 YPEPETFNPDRWAPNGLAEQ 444
>SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 675
Score = 25.8 bits (54), Expect = 6.1
Identities = 9/22 (40%), Positives = 17/22 (77%)
Frame = +3
Query: 90 LLNNGIELLAYRRPFQFHQDRW 155
+ ++G+E++AYR P+Q H + W
Sbjct: 194 IADDGLEIMAYRIPYQ-HPEFW 214
>SPAC2C4.07c |||ribonuclease II |Schizosaccharomyces pombe|chr
1|||Manual
Length = 927
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +1
Query: 424 TTEFKVLKKQKKRNDIPKVNNEEPNLSSELRVNEFNIQMISKNI 555
T + KV K KK+N K++ + V++ ++ +SKN+
Sbjct: 92 TEKAKVKPKAKKKNSKEKISKSSKQDEHKTDVHKESVSKLSKNL 135
>SPAC31A2.10 |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 460
Score = 25.4 bits (53), Expect = 8.1
Identities = 20/63 (31%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Frame = +1
Query: 442 LKKQKKRNDIPKVNNEEPNLSSELRVNEFNIQMISKNIYEQLFK-KPSSSPDPEVIKSCQ 618
L+K + N + ++E +LS L +EF+I +KNI + + SS P E I +
Sbjct: 347 LEKYFEVNALDISDSESEDLSDILSDSEFSIGNRNKNIGNNEYSAQISSDPAEEEIANAM 406
Query: 619 ESL 627
SL
Sbjct: 407 HSL 409
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 25.4 bits (53), Expect = 8.1
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = +1
Query: 469 IPKVNNEEPNLSSELRVNEFNIQMISKNIYEQLFKK--PSSSPDPEVIKSCQES 624
+P+V ++L + N ++S IYE L++K + EV KS +ES
Sbjct: 315 VPEVQTTNIGYQTDLPLQNPNDNLVSLAIYEALYEKFLKACKDLEEVSKSYEES 368
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,733,887
Number of Sequences: 5004
Number of extensions: 54839
Number of successful extensions: 180
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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