Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_K20
(741 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 55 9e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 3e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 4e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.006
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.6
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.1
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 26 6.5
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.5
SPCC18B5.07c |nup61||nucleoporin Nup61|Schizosaccharomyces pombe... 25 8.6
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 25 8.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 55.2 bits (127), Expect = 9e-09
Identities = 29/78 (37%), Positives = 43/78 (55%)
Frame = +3
Query: 276 TAKQRKTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCM 455
T RK K E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA- 130
Query: 456 DPEDDDGMIPYAAFLKKV 509
D DG+I Y F + +
Sbjct: 131 -DTDGDGVINYEEFSRVI 147
Score = 29.1 bits (62), Expect = 0.70
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +3
Query: 318 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 497
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 498 L 500
L
Sbjct: 71 L 71
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 43.6 bits (98), Expect = 3e-05
Identities = 22/67 (32%), Positives = 41/67 (61%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+ Y
Sbjct: 77 EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVNYHD 133
Query: 495 FLKKVMA 515
F++ ++A
Sbjct: 134 FVQMILA 140
Score = 25.4 bits (53), Expect = 8.6
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +2
Query: 152 STLRDLLRALNSNPTLATI 208
+++ DLLRA NPTLA I
Sbjct: 26 TSIGDLLRACGQNPTLAEI 44
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 43.2 bits (97), Expect = 4e-05
Identities = 21/75 (28%), Positives = 44/75 (58%)
Frame = +3
Query: 291 KTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 470
K + + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP +
Sbjct: 70 KLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TN 127
Query: 471 DGMIPYAAFLKKVMA 515
G Y F++++MA
Sbjct: 128 SGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.23
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +3
Query: 315 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 494
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.9 bits (79), Expect = 0.006
Identities = 21/74 (28%), Positives = 37/74 (50%)
Frame = +3
Query: 228 RRRARSCSHSKSSFPSTAKQRKTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALG 407
+RR+R+ S + + A R T +D E KL+D +++ + EL + ALG
Sbjct: 8 KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Query: 408 EKLDDSEVAEVTKD 449
+ SEV ++ +D
Sbjct: 68 FNAEKSEVLKILRD 81
Score = 27.9 bits (59), Expect = 1.6
Identities = 19/74 (25%), Positives = 31/74 (41%)
Frame = +3
Query: 291 KTKTRXAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDD 470
K R E+ +L+D +E G + L L E +DD E+ + ++ D
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLDQ 159
Query: 471 DGMIPYAAFLKKVM 512
DG I F+ +M
Sbjct: 160 DGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.6
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -3
Query: 664 APPEELSPPRALPAPVPQSRASVF*GPSHRT 572
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.5 bits (58), Expect = 2.1
Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +1
Query: 64 MSDLSKNDVER-ASFAFSIYDFEGKGKIDAFNLARSPES 177
+++L DV R SF F +YDF G G +D ++ + E+
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMDKPDVLKVSEA 643
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 6.5
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +3
Query: 228 RRRARSCSHSKSSFPSTAKQRKTKTRXAYE-DFLECLKLYDKNENGLM 368
RR+ S SH +SF + + +K R ++ D L+ L + + E+G++
Sbjct: 36 RRKHASHSHDDASFFDPSNEEASKLRESFAMDTLDALLQWFEEEDGVV 83
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.5
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 402 LGEKLDDSEVAEVTKDCMDPED 467
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPCC18B5.07c |nup61||nucleoporin Nup61|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 565
Score = 25.4 bits (53), Expect = 8.6
Identities = 26/105 (24%), Positives = 44/105 (41%), Gaps = 1/105 (0%)
Frame = +1
Query: 16 TSASEDREGTRKTPHKMSDLSKNDVERASFAFSIYDFE-GKGKIDAFNLARSPESAQLKP 192
TS+++ GT T + S N FAF+ + E K F+ + + SA
Sbjct: 244 TSSNQTATGTAATT--TNQFSFNTAANP-FAFAKKENEESKPLTPVFSFSTTMASADASK 300
Query: 193 HTGNHRETRWYKEEGREAAHTRRVPSHLQPSKERQRPGXRMKISW 327
T ET+ K E + ++ + + ++P+K G M SW
Sbjct: 301 ETKQTHETKDSKSEESKPSNNEKSENAVEPAK-----GNTMSFSW 340
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 25.4 bits (53), Expect = 8.6
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = -2
Query: 179 ALSGDRARLKASILPLPSKS*IEKAKDALSTSFLLRSLILCGVFLVPSRSSLADV 15
+LS + + S +P S S + +ALS++ L S +PS SS +V
Sbjct: 536 SLSSSTSSVSTSYIPNASSSVYASSTEALSSNSLSSSTSSASTSYIPSASSSYEV 590
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,627,886
Number of Sequences: 5004
Number of extensions: 46217
Number of successful extensions: 145
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 145
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 351258950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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