Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_H11
(812 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 103 3e-23
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 98 1e-21
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 86 6e-18
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 59 6e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 59 8e-10
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.016
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.26
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.79
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.4
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 3.2
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 5.5
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 103 bits (247), Expect = 3e-23
Identities = 53/134 (39%), Positives = 81/134 (60%)
Frame = +3
Query: 228 FQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 407
++ AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 408 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDS 587
+ +F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 588 QGNINYENFVHLIM 629
G +NY +FV +I+
Sbjct: 126 DGMVNYHDFVQMIL 139
Score = 26.6 bits (56), Expect = 4.2
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 225 EFQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDV 338
EF FQ+FD G I V ++ L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 98.3 bits (234), Expect = 1e-21
Identities = 54/140 (38%), Positives = 81/140 (57%), Gaps = 3/140 (2%)
Frame = +3
Query: 216 QLAEFQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--ISFEV 389
Q+AEF+ AF LFD DG I ++G +R+LGQ+PT ++++ + D I F
Sbjct: 10 QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTE 69
Query: 390 FLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL 569
FL + K + D + E + FDKDGNG+I+ EL H+L++LGE+LS +EV ++
Sbjct: 70 FLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMI 127
Query: 570 -QGQEDSQGNINYENFVHLI 626
+ D G INYE F +I
Sbjct: 128 READTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 85.8 bits (203), Expect = 6e-18
Identities = 49/142 (34%), Positives = 81/142 (57%), Gaps = 1/142 (0%)
Frame = +3
Query: 207 SXXQLAEFQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFE 386
S Q E + AF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 387 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQL 566
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 567 LQ-GQEDSQGNINYENFVHLIM 629
+Q + G+ +Y +FV IM
Sbjct: 120 VQEADPTNSGSFDYYDFVQRIM 141
Score = 34.7 bits (76), Expect = 0.016
Identities = 16/65 (24%), Positives = 34/65 (52%)
Frame = +3
Query: 225 EFQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 404
E+ AF++FD G I A+ D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 405 QAISK 419
+ ++K
Sbjct: 139 RIMAK 143
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 59.3 bits (137), Expect = 6e-10
Identities = 41/141 (29%), Positives = 64/141 (45%), Gaps = 3/141 (2%)
Frame = +3
Query: 216 QLAEFQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDER--ISFEV 389
Q + AF+LFDS D I ++ A+RALG N +S+V K + + E
Sbjct: 35 QRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMED 94
Query: 390 FLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL 569
F+ + K D + FD D G IS LR + L E + D E+E ++
Sbjct: 95 FVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMI 152
Query: 570 QGQE-DSQGNINYENFVHLIM 629
+ + D G IN + F+ ++M
Sbjct: 153 EEFDLDQDGEINEQEFIAIMM 173
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 58.8 bits (136), Expect = 8e-10
Identities = 41/137 (29%), Positives = 63/137 (45%)
Frame = +3
Query: 216 QLAEFQXAFQLFDSRGDGKIHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFL 395
Q+ E + AF L D GDG I + L +L Q+ +E + + P I+ FL
Sbjct: 46 QIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--INLAAFL 103
Query: 396 PIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG 575
++ S ND +E FD +G I + +R LS++G+++ EVE +L+
Sbjct: 104 TAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRS 161
Query: 576 QEDSQGNINYENFVHLI 626
S G YE FV I
Sbjct: 162 Y-TSHGVFYYEKFVDAI 177
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.016
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +3
Query: 462 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 632
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.26
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +3
Query: 468 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 623
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.79
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -1
Query: 560 LYLIITKLLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 390
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 2.4
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +3
Query: 384 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 551
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 552 EVEQLL 569
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 3.2
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +3
Query: 492 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 635
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 26.2 bits (55), Expect = 5.5
Identities = 16/67 (23%), Positives = 32/67 (47%)
Frame = -1
Query: 728 LTVSHYGSNCMVTDLVSLQIKVLQQMMT*NSALHDEVNKVLIVDISLRVFLSLQELLYLI 549
L S+Y ++ D V Q+K L Q +S + + + + RV S+ + L+L+
Sbjct: 539 LETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLL 598
Query: 548 ITKLLSE 528
+ ++ E
Sbjct: 599 VNRIKQE 605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,886,872
Number of Sequences: 5004
Number of extensions: 52074
Number of successful extensions: 166
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 396433620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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