Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_F12
(787 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 265 3e-71
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 262 2e-70
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 165 4e-41
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 147 1e-35
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 83 2e-16
SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0) 33 0.20
SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.8
SB_10602| Best HMM Match : RVT_1 (HMM E-Value=1.3e-10) 30 1.8
SB_42068| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.4
SB_430| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.2
SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.2
SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.6
SB_47927| Best HMM Match : TSP_1 (HMM E-Value=9.5e-34) 28 7.5
SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) 28 7.5
SB_12316| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 9.9
>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 309
Score = 265 bits (649), Expect = 3e-71
Identities = 134/199 (67%), Positives = 152/199 (76%), Gaps = 2/199 (1%)
Frame = +3
Query: 195 MAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKE 374
MA AMKE TE L EERNLLSVAYKNVVGA+RSSWRVISSIEQK EGSERK+Q +
Sbjct: 1 MAKAMKEATEISETLEQEERNLLSVAYKNVVGAKRSSWRVISSIEQKLEGSERKKQNTET 60
Query: 375 YRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 554
YR +E EL E+C VL LL+ LIP A + ESKVFYLKMKGDYYRY EVA + R V
Sbjct: 61 YRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVAGADRRREV 120
Query: 555 VEDSQKAYQDAFEISK--AKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDA 728
V+ + KAY +A EI++ K+ PT PIRLGLALNFSVFYYEI+ +AC LAK+AFDDA
Sbjct: 121 VQKAMKAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDA 180
Query: 729 IAELDTLNEXSYKDSTLIM 785
IAELDTL+E YKDSTLIM
Sbjct: 181 IAELDTLSEDQYKDSTLIM 199
Score = 106 bits (255), Expect = 2e-23
Identities = 53/82 (64%), Positives = 67/82 (81%), Gaps = 3/82 (3%)
Frame = +3
Query: 549 SVVEDSQKAYQDAFEISKA---KMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAF 719
+VVE + +AY++A E ++ K+ PT PIRLGLALNFSVF+YEI + ++AC+LAKQAF
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAF 266
Query: 720 DDAIAELDTLNEXSYKDSTLIM 785
DDAIAELD+LNE YKDSTLIM
Sbjct: 267 DDAIAELDSLNEDQYKDSTLIM 288
>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
Length = 248
Score = 262 bits (642), Expect = 2e-70
Identities = 133/227 (58%), Positives = 165/227 (72%), Gaps = 3/227 (1%)
Frame = +3
Query: 114 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGA 293
P+ +EEL+ AK+AEQAERYDDM AM VT+ G L++EERNLLSVAYKNVVGA
Sbjct: 3 PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYKNVVGA 62
Query: 294 RRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKAS---N 464
RRSSWRVISS+EQK E + K+YR + EL C +VL +L+ +L+ N
Sbjct: 63 RRSSWRVISSMEQK--APEEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDIN 120
Query: 465 PESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLA 644
E+KVFYLKM+GDY+RYL EVA G++R +E S++AY+DA ++ P+HPIRLGLA
Sbjct: 121 TEAKVFYLKMRGDYHRYLVEVAEGDSRKENIEKSREAYKDA-SAKAEELSPSHPIRLGLA 179
Query: 645 LNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEXSYKDSTLIM 785
LNFSVFYYEI N P +AC+LAK+AFDDAIA LD L + SYKDSTLIM
Sbjct: 180 LNFSVFYYEIENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIM 226
>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 219
Score = 165 bits (401), Expect = 4e-41
Identities = 81/138 (58%), Positives = 107/138 (77%)
Frame = +3
Query: 126 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 305
M V +E L+ AKL+EQ +RYD+MA MKEV+E +LS EERNLLSV+YKN+VG RRSS
Sbjct: 80 MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYKNIVGQRRSS 139
Query: 306 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFY 485
WRVISSIE+KT S + K+Y+ +EKEL+++C +VLG+L++ LIP A + E+KVFY
Sbjct: 140 WRVISSIEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFY 197
Query: 486 LKMKGDYYRYLAEVATGE 539
K+KGDYYRYLAE + G+
Sbjct: 198 FKLKGDYYRYLAEFSHGQ 215
>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
Length = 251
Score = 147 bits (356), Expect = 1e-35
Identities = 75/123 (60%), Positives = 93/123 (75%), Gaps = 3/123 (2%)
Frame = +3
Query: 135 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 314
DKEE V AKLAEQAERYDDM +MKEV + G ELS E+RNLLSVAYKNV+GARR+SWR+
Sbjct: 3 DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYKNVIGARRASWRI 62
Query: 315 ISSIEQKTE--GSE-RKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFY 485
I+SIEQK E G + K +M + YR +E+EL+ IC ++L LLD LI + + ESKVFY
Sbjct: 63 ITSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFY 122
Query: 486 LKM 494
K+
Sbjct: 123 NKI 125
>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 607
Score = 83.0 bits (196), Expect = 2e-16
Identities = 43/102 (42%), Positives = 63/102 (61%), Gaps = 2/102 (1%)
Frame = +3
Query: 126 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 305
M + ELVQ AKLAEQ ER++D+ MK+ E L+ E RNLLSV YKNVVG++R +
Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYKNVVGSKRFA 245
Query: 306 WRVISSIEQKTEGSERKQQMAK--EYRVKVEKELREICYDVL 425
WR + ++ R Q+ +Y+ K+E EL+ +C ++L
Sbjct: 246 WRHLHHDALQSGRYIRDSQLKGIIKYKEKIEMELKTLCREIL 287
>SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0)
Length = 442
Score = 33.5 bits (73), Expect = 0.20
Identities = 23/86 (26%), Positives = 40/86 (46%)
Frame = +3
Query: 138 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 317
KE+ AK ++ ER + A K+ E + EE++ L K R+ +
Sbjct: 339 KEKERLEAKQKKEQERLEKQAEKEKKEKERLEKKQREEKDRLEKKEKKEEEKRKKEEEIN 398
Query: 318 SSIEQKTEGSERKQQMAKEYRVKVEK 395
+ IE+K + E+K+Q +E K E+
Sbjct: 399 AKIEEKKKREEKKKQEEEEKMKKKEQ 424
>SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 994
Score = 30.3 bits (65), Expect = 1.8
Identities = 16/51 (31%), Positives = 28/51 (54%)
Frame = +3
Query: 102 ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEER 254
+SPLPS+ +E+V KLAE+ E ++++ T+ E + EE+
Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693
>SB_10602| Best HMM Match : RVT_1 (HMM E-Value=1.3e-10)
Length = 416
Score = 30.3 bits (65), Expect = 1.8
Identities = 16/62 (25%), Positives = 31/62 (50%)
Frame = +3
Query: 420 VLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEIS 599
+LGL++ H+ PK+ + ++F +Y+ +L V T+ +E + Y + S
Sbjct: 116 ILGLIESHIDPKSFH--HRMFLAACCLEYFGFLRSVEFTVTQEKCIEQNMPLYAVFIDFS 173
Query: 600 KA 605
KA
Sbjct: 174 KA 175
>SB_42068| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 3367
Score = 29.9 bits (64), Expect = 2.4
Identities = 28/121 (23%), Positives = 55/121 (45%), Gaps = 12/121 (9%)
Frame = +3
Query: 123 TMSVDKEELVQRAKLAEQAERY---DDMAAAMKEVTETGVELSNE-ERNLLSVAYKNVVG 290
++S+ +EL + LA Q + D A K+VT ++ + E R + +
Sbjct: 3123 SLSLKSQELEAKNALANQKLKQMVKDQQEAEKKKVTSMEIQTTIETHRKQTIIEMVSCAA 3182
Query: 291 ARRSSWR--------VISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHL 446
R S++ + SS++++T+ + KQQ + +VE + E V G+ +HL
Sbjct: 3183 CLRKSYQESAFLDLLITSSLQKQTKQIKEKQQAVMKDLAQVEPAVDEARQAVKGIKKQHL 3242
Query: 447 I 449
+
Sbjct: 3243 V 3243
>SB_430| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2202
Score = 29.5 bits (63), Expect = 3.2
Identities = 22/100 (22%), Positives = 52/100 (52%), Gaps = 3/100 (3%)
Frame = +3
Query: 114 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLL-SVAYKNVVG 290
PSS S DK+E+ +++ +++++ ++A K + E ++S + S+ K +
Sbjct: 1066 PSSRSSHDKDEISEKSNPSDKSD--VEVARKNKHMPEALYKISETISGMNDSITLKEPLK 1123
Query: 291 ARRSSWR--VISSIEQKTEGSERKQQMAKEYRVKVEKELR 404
A+ R ++ + E +R+ + K++R++ EK+ R
Sbjct: 1124 AKDGDGRNKEEQELKDRMENEKREDTIRKQHRLQWEKKAR 1163
>SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1234
Score = 29.5 bits (63), Expect = 3.2
Identities = 27/115 (23%), Positives = 52/115 (45%), Gaps = 6/115 (5%)
Frame = +3
Query: 138 KEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGAR------R 299
++ L + K A +R +A+A+ + + + LS K VV + +
Sbjct: 279 QKTLSEMFKQAVTEDRPKMLASAITKALNQDIHSPSALNLPLSTPAKQVVKTKWDRIREK 338
Query: 300 SSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASN 464
W ++S E+ +ER++Q E +VK E++ R+ ++ + HL K SN
Sbjct: 339 HQWNLLSEDEKNKIKAERRKQKRLELKVKREEKERKRLEELKRVSFAHLGMKLSN 393
>SB_13504| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 4924
Score = 28.7 bits (61), Expect = 5.6
Identities = 19/53 (35%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Frame = +3
Query: 528 ATGETRHS-VVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 683
A GETR + D+ +A +D KA+ Q + ALN S+F++E++NS
Sbjct: 4020 ALGETRRKRSLVDTPEANED-----KAQHQVPDAEKCQFALNASLFFFEVINS 4067
>SB_47927| Best HMM Match : TSP_1 (HMM E-Value=9.5e-34)
Length = 512
Score = 28.3 bits (60), Expect = 7.5
Identities = 20/85 (23%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
Frame = +3
Query: 156 RAKLAEQAERYDDMAAAM-KEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQ 332
+ K+ +++E+YD + + KE T+ G + E + N AR + ++ +
Sbjct: 13 KRKIFKKSEKYDRLEEPLEKEGTDGGEIEAEAEVEPHTEEEANYNEARSENCEKNNNNDS 72
Query: 333 KTEGSERKQQMAKEYRVKVEKELRE 407
T ++ +Q KE+ V +EK+L++
Sbjct: 73 STPDAKSQQADIKEWIVSLEKQLKD 97
>SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1126
Score = 28.3 bits (60), Expect = 7.5
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +3
Query: 309 RVISSIEQKTEGSERKQQMAKEYRVKVEKELREIC 413
R++ IE+K E E ++ AKE + + E+E + IC
Sbjct: 919 RIMKEIEEK-EKKEEAERKAKEEKEREERERKRIC 952
>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
Length = 783
Score = 28.3 bits (60), Expect = 7.5
Identities = 11/34 (32%), Positives = 22/34 (64%)
Frame = +3
Query: 354 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 455
K++M ++Y K+EKE+ Y+++ L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325
>SB_12316| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 676
Score = 27.9 bits (59), Expect = 9.9
Identities = 26/99 (26%), Positives = 47/99 (47%)
Frame = +3
Query: 111 LPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVG 290
L S+ +D+E R +++++ RY + A MK V E + ++E + +
Sbjct: 539 LDSNLSKLDQEVRGLREEISQEESRYHYLHAMMK-VLEIQQKRIDDEMKAYTAQDQ---A 594
Query: 291 ARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELRE 407
R+ SWR EQ T + ++ + K R K +K +RE
Sbjct: 595 DRKKSWR-----EQYTRKIQEQENLGKSLREK-QKAVRE 627
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,269,812
Number of Sequences: 59808
Number of extensions: 496719
Number of successful extensions: 1415
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1306
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1408
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2155861620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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