Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_D03
(719 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 86 5e-18
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 38 0.002
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 29 0.88
SPBC9B6.09c |mdl1||mitochondrial peptide-transporting ATPase|Sch... 28 1.5
SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr 1... 26 4.7
SPBC3D6.02 |but2||But2 family protein But2 |Schizosaccharomyces ... 25 8.2
>SPCC663.03 |pmd1||leptomycin efflux transporter
Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1362
Score = 85.8 bits (203), Expect = 5e-18
Identities = 42/148 (28%), Positives = 84/148 (56%)
Frame = +3
Query: 264 ITVGSIAAFLQGACFPVFALLFGFSSGIFILDNRDDIIYLADLYSVLFVVIAAVAGISMC 443
+ +G +A+ + GA +PV A +F IF + D ++ ++++V ++++A V +
Sbjct: 795 LLIGILASMICGAAYPVQAAVFARFLNIFTDLSSTDFLHKVNVFAVYWLILAIVQFFAYA 854
Query: 444 LQSTTFTSAGLKMTTRLRHQYFASLLKQEIGFFDKETNTVGAICARLSGDTAEVQGATGL 623
+ + T A + R+R+ F +LL+Q++ FFD+ NTVGAI LS ++G +G
Sbjct: 855 ISNFAMTYAMEAVLQRIRYHLFRTLLRQDVEFFDRSENTVGAITTSLSTKIQSLEGLSGP 914
Query: 624 RIGLILQGISSVVVGFLLAISYNWKLTL 707
+G Q +++++ +L+++ WKL L
Sbjct: 915 TLGTFFQILTNIISVTILSLATGWKLGL 942
Score = 68.9 bits (161), Expect = 7e-13
Identities = 47/161 (29%), Positives = 81/161 (50%), Gaps = 7/161 (4%)
Frame = +3
Query: 246 APEWPLIT--VGSIAAFLQGACFPVFALLFGFSSGIF--ILDNR--DDIIYLADLYSVLF 407
A +W ++ G+I G P+ +L+ G + F + + + D + + F
Sbjct: 87 ADKWDIMLQLAGTITGIGAGLGMPLMSLVSGQLAQAFTDLASGKGASSFQHTVDHFCLYF 146
Query: 408 VVIA-AVAGISMCLQSTTFTSAGLKMTTRLRHQYFASLLKQEIGFFDKETNTVGAICARL 584
+ IA V G S + + TF AG ++ R+R Y ++L Q IG+FD+ G I R+
Sbjct: 147 IYIAIGVFGCSY-IYTVTFIIAGERIARRIRQDYLHAILSQNIGYFDRLG--AGEITTRI 203
Query: 585 SGDTAEVQGATGLRIGLILQGISSVVVGFLLAISYNWKLTL 707
+ DT +Q G ++GL+ I++ V GF++A +WK TL
Sbjct: 204 TTDTNFIQDGLGEKVGLVFFAIATFVSGFVIAFIRHWKFTL 244
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 37.5 bits (83), Expect = 0.002
Identities = 34/161 (21%), Positives = 68/161 (42%)
Frame = +3
Query: 219 SDWQLLKLNAPEWPLITVGSIAAFLQGACFPVFALLFGFSSGIFILDNRDDIIYLADLYS 398
S W++ KL W +G + + +QGA P+FA + +F+ D I +A +S
Sbjct: 770 SIWKVKKLR---W-FFLLGLLTSLIQGASVPIFAYVISKCLNLFM--QIDPSIGVA-FWS 822
Query: 399 VLFVVIAAVAGISMCLQSTTFTSAGLKMTTRLRHQYFASLLKQEIGFFDKETNTVGAICA 578
+ +V+AA +G S F+ + R L Q+ +FD+ N +
Sbjct: 823 SMVLVVAAGSGASYFFSHYIFSISAKIWCDHYRLLAVKVLFTQDQAWFDQIENYPLVLSK 882
Query: 579 RLSGDTAEVQGATGLRIGLILQGISSVVVGFLLAISYNWKL 701
L + ++++ I + + ++G + + W+L
Sbjct: 883 ILVNNISDMRNMISSLIEEVFIAFTMAIIGIAWSFATGWRL 923
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 28.7 bits (61), Expect = 0.88
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = -2
Query: 652 LIPCSMRPILSPVAPCTSAVSPLNLAQIAPTVFVSLSKNPISCFNKDA 509
++PC RPI S V ++ + LA + S NP C+N D+
Sbjct: 1102 IVPCYDRPISSRVNSIIEDIARIGLAPNKVKLAYSHPINPFVCWNADS 1149
>SPBC9B6.09c |mdl1||mitochondrial peptide-transporting
ATPase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 726
Score = 27.9 bits (59), Expect = 1.5
Identities = 18/76 (23%), Positives = 39/76 (51%)
Frame = +3
Query: 477 KMTTRLRHQYFASLLKQEIGFFDKETNTVGAICARLSGDTAEVQGATGLRIGLILQGISS 656
++ +RLR + FA + + FFD + G + +RL+ D++ V + + + L+ S
Sbjct: 232 RIVSRLRARLFAKCMSLDGAFFDFHKH--GDLISRLTTDSSIVGKSLSMYLSDGLRSSVS 289
Query: 657 VVVGFLLAISYNWKLT 704
+ G + + + +LT
Sbjct: 290 AIAGIGMMLYVSMRLT 305
>SPAC23G3.12c |||serine protease |Schizosaccharomyces pombe|chr
1|||Manual
Length = 996
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = -2
Query: 619 PVAPCTSAVSPLNLAQIAPTVFVSLSKNPISCFNKDAKY 503
P PCT+++ LN+ PT N I+CF K Y
Sbjct: 504 PSKPCTTSIPKLNVENFGPTA------NIINCFVKVLYY 536
>SPBC3D6.02 |but2||But2 family protein But2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 8.2
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +2
Query: 479 NDNSTKTSIFCIFVETGNRVFR*RNEYGRRDLCEIER*HC*GTGCYWTQDRP 634
ND S + F ++ +GN + + G L E+ R +C GC +TQ+ P
Sbjct: 298 NDGSAQ---FVVYNMSGNPKKQTTSSKGPTRLYEVARFNCTTRGCEYTQNIP 346
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,793,984
Number of Sequences: 5004
Number of extensions: 54682
Number of successful extensions: 152
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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