Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_B18
(758 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 105 9e-24
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 72 8e-14
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 45 1e-05
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 30 0.41
SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyce... 29 0.54
SPBC106.07c |||N alpha-acetyltransferase Nat2 |Schizosaccharomyc... 27 2.2
SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces ... 26 6.7
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 26 6.7
SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 8.9
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 105 bits (251), Expect = 9e-24
Identities = 57/186 (30%), Positives = 101/186 (54%), Gaps = 4/186 (2%)
Frame = +2
Query: 140 LSKSVELTFELPDSAEXCFY-QTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQE 316
L+ ++ELTF+L + + C+Y + Y V SGG +DV I+AP+++ + +
Sbjct: 17 LASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPSQKTVALGK 76
Query: 317 KMQYDSHQFTALASGDYKVCFSNEFSTFSHKLVYMELNVGPEQPLPGV-GDHATVLTQ-- 487
K + FT G+Y+ CF N STF+ K+V ME+ + E LP + D A +
Sbjct: 77 KRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDEAKDYKKDS 136
Query: 488 LETSAEEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVM 667
++++ EI +AL+ I Q + + RE + + ++FW+S+ E++ +V ++ QV
Sbjct: 137 MQSTVLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVVALSALQVF 196
Query: 668 ILKNFF 685
I+K FF
Sbjct: 197 IVKTFF 202
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 72.1 bits (169), Expect = 8e-14
Identities = 46/169 (27%), Positives = 74/169 (43%), Gaps = 1/169 (0%)
Frame = +2
Query: 191 CFYQTMDKDTAASLEYQVISGGQYDVSVKIEAPNRQIIYQQEKMQYDSHQFTALASGDYK 370
CFY+ + + S+ YQ GG VS+ I P QI++Q+ + FT G Y
Sbjct: 33 CFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKYM 92
Query: 371 VCFSNEFSTFSHKLVYMELNVGPEQPLPGVGDH-ATVLTQLETSAEEIHSALNRIIDHQT 547
CF N+ K V ++ + + D L ++H ++++ Q
Sbjct: 93 YCFYNDALDGESKEVLFNVH-----GVIYISDEDLDANNPLLGKVRQLHDTISKVKHEQE 147
Query: 548 HHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNFFSDK 694
+ RE R AE N++V WWS+ +T+ +V V Q+ LK F K
Sbjct: 148 YFVARERIHRNTAESTNDRVKWWSILQTVILVSVCVFQIFYLKRLFEVK 196
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 44.8 bits (101), Expect = 1e-05
Identities = 25/134 (18%), Positives = 59/134 (44%), Gaps = 2/134 (1%)
Frame = +2
Query: 290 NRQIIYQQEKMQYDSHQFTALASGDYKVCF--SNEFSTFSHKLVYMELNVGPEQPLPGVG 463
N +IY+ + ++ +FT G +++C+ +++ + FS + +++ + +
Sbjct: 76 NNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKTRLHVDLASDDKYNLIA 135
Query: 464 DHATVLTQLETSAEEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIV 643
L +++ L I+ Q R RE R +E N +V W++ + + ++
Sbjct: 136 SSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTIVQIVVLL 195
Query: 644 CVAFTQVMILKNFF 685
Q+ L+ FF
Sbjct: 196 VTCIWQLSHLQRFF 209
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 29.9 bits (64), Expect = 0.41
Identities = 28/124 (22%), Positives = 48/124 (38%), Gaps = 6/124 (4%)
Frame = +2
Query: 341 FTALASGDYKVCFSNEF------STFSHKLVYMELNVGPEQPLPGVGDHATVLTQLETSA 502
F AS +CF N S + V +E VG + A L +E
Sbjct: 89 FDVDASAMLDICFLNTLTPGAIESEHKKRSVKLEFTVGADADDYSSLQKANNLEPVEADI 148
Query: 503 EEIHSALNRIIDHQTHHRLREAQGRKRAEDLNEKVFWWSMGETLAIVCVAFTQVMILKNF 682
+ I + + REA+ R E NE+V ++ +++ + Q++ L++F
Sbjct: 149 RRARDFIEEIKGKIYYLQAREARFRNTNESTNERVKNFAYLTFISLFVLVIWQILYLRSF 208
Query: 683 FSDK 694
F K
Sbjct: 209 FQRK 212
>SPBC4C3.06 |||actin cytoskeletal protein Syp1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 818
Score = 29.5 bits (63), Expect = 0.54
Identities = 28/100 (28%), Positives = 43/100 (43%), Gaps = 7/100 (7%)
Frame = -3
Query: 480 RTVA*SPTPGRGCSGPTFSSIYTSLCENVLNSLLKHTL*SPDARAVNWCESYCIFSC*YI 301
R+ SPT PT + + E V N+L K+ S R + Y S Y+
Sbjct: 460 RSTTTSPTSAVASPAPTENEDSNAAIERVANTLRKNPTISRRTRRAGTMDRYATASSDYM 519
Query: 300 ICLLGA--SIFTLTSYCPPE--ITW---YSNEAAVSLSIV 202
LG+ ++ TL+ P+ TW + N + +S SIV
Sbjct: 520 ESNLGSLPNLSTLSLQSGPDSTATWHPEFPNSSGLSASIV 559
>SPBC106.07c |||N alpha-acetyltransferase Nat2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 167
Score = 27.5 bits (58), Expect = 2.2
Identities = 10/32 (31%), Positives = 19/32 (59%)
Frame = +2
Query: 578 KRAEDLNEKVFWWSMGETLAIVCVAFTQVMIL 673
+R ++L +K WWS+G + I + F+ +L
Sbjct: 52 QRVKELTKKYGWWSLGVYIGISVLDFSASFVL 83
>SPBC12C2.01c ||SPBC17F3.03c|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 318
Score = 25.8 bits (54), Expect = 6.7
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +1
Query: 613 VVYG*NLGYCLCCIYTSYDTKEF 681
+V N+G C CC + Y TK+F
Sbjct: 65 IVSNHNVG-CSCCYFRQYSTKQF 86
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 25.8 bits (54), Expect = 6.7
Identities = 25/107 (23%), Positives = 52/107 (48%), Gaps = 4/107 (3%)
Frame = +2
Query: 62 KGFXINAKMFXAPLXFTXLITIINGVLSKSVELTFELPDSAEXCFYQTMDKDTAASLEYQ 241
KG+ + K+F L L +I+N + + +++ +P++ + +T+ AA L
Sbjct: 45 KGYDVLIKVFVNKLPEISLSSIVNLLKEEQENISYRVPNAVP--YIKTLVTLRAAYLVRP 102
Query: 242 VISGGQYD-VSVK--IEAPNRQ-IIYQQEKMQYDSHQFTALASGDYK 370
++ YD +S + +E ++ I++Q K D H+ + GD K
Sbjct: 103 YVTHNLYDRISTRPFLELTEKKWIMFQLLKGISDCHRL-GVCHGDIK 148
>SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 865
Score = 25.4 bits (53), Expect = 8.9
Identities = 11/45 (24%), Positives = 21/45 (46%)
Frame = +3
Query: 288 LTDKLYISKRRCNMIHTSSPLWRLVITKCVLVMSLVHFHINLCIW 422
LT K + + + WR V+ + +L + L + + LC+W
Sbjct: 613 LTQKYILMYVSNSSTESGGGYWRPVVNRILLGLELANIILFLCLW 657
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,835,576
Number of Sequences: 5004
Number of extensions: 56455
Number of successful extensions: 152
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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