Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_B15
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 145 7e-36
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 130 2e-31
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 124 1e-29
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 110 2e-25
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 103 4e-23
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 83 4e-17
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 40 5e-04
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 39 9e-04
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 32 0.074
SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 27 2.1
SPBC215.11c |||aldo/keto reductase, unknown biological role|Schi... 27 2.8
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c... 26 4.9
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 26 4.9
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 145 bits (351), Expect = 7e-36
Identities = 83/209 (39%), Positives = 124/209 (59%), Gaps = 11/209 (5%)
Frame = +2
Query: 119 GXPLPXVALGTGRGTAKXSDSIXXVRQAVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAI 298
G +P + LGT R + + AV A++ GYRHID AA+Y +E++VG GI E+
Sbjct: 21 GSKIPGLGLGTWRSEPNQT------KNAVKTALQYGYRHIDAAAIYGNEDEVGDGIKES- 73
Query: 299 ANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPIATK------ 460
G V R++++VT+KLW + HA + V AL+++LK L LDY+D YLIH+P++ K
Sbjct: 74 --G-VPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEYLIHWPVSFKTGEDKF 130
Query: 461 PDDSPDNIDYL-----ETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQI 625
P D N+ Y ETW+ M+ + G R IG+SNFN T + R++ + ++P ++Q+
Sbjct: 131 PKDKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLERILKVAKVKPAVHQM 190
Query: 626 EVNPTNTQEPLXAHCXSLGIAVMAYSPFG 712
E++P Q LGI V AYSPFG
Sbjct: 191 ELHPFLPQTEFVEKHKKLGIHVTAYSPFG 219
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 130 bits (315), Expect = 2e-31
Identities = 78/207 (37%), Positives = 120/207 (57%), Gaps = 11/207 (5%)
Frame = +2
Query: 119 GXPLPXVALGTGRGTAKXSDSIXXVRQAVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAI 298
G +P + LGT R + + AV A++AGYRHIDTA +Y +E+++G+GI E+
Sbjct: 20 GDKIPSIGLGTWRSGKDET------KNAVCAALKAGYRHIDTAHIYGNEKEIGEGIRES- 72
Query: 299 ANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPIA--TKPDDS 472
G V R +++VT+KLW + H V AL+++L+ L L+YID YLIH+P A + P++
Sbjct: 73 --G-VPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAYLIHWPFALLSGPEEL 129
Query: 473 PDN---------IDYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQI 625
P N + ETWQ M++ + G R IG+SNFN + R++ + ++P I+Q+
Sbjct: 130 PRNEKGELIYEDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTIHQM 189
Query: 626 EVNPTNTQEPLXAHCXSLGIAVMAYSP 706
E++P Q L I V AYSP
Sbjct: 190 ELHPYLPQTEYLEKHKKLQIHVSAYSP 216
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 124 bits (300), Expect = 1e-29
Identities = 77/201 (38%), Positives = 106/201 (52%), Gaps = 2/201 (0%)
Frame = +2
Query: 125 PLPXVALGTGRGTAKXSDSIXXVRQAVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAIAN 304
P+P +GT + + + +V A+ AG+ HID A VY +EE+VG + EA
Sbjct: 11 PVPAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVYGNEEEVGVALKEAN-- 68
Query: 305 GLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPIATKPDDSPDNI 484
V R +LF+T+K+ H D + AL ESL+KLG DY+DLYL+H PI P +
Sbjct: 69 --VPRSKLFITSKVM---HNVDNIPEALNESLRKLGTDYLDLYLLHSPIPFYEKKIPIS- 122
Query: 485 DYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEVNPT--NTQEPL 658
E W+ M+ A GL S+GVSNF + L+ S I P +NQIE +P +PL
Sbjct: 123 ---EGWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHPQVYKAAKPL 179
Query: 659 XAHCXSLGIAVMAYSPFGFVV 721
C S GI V Y P +V
Sbjct: 180 VEFCQSKGIIVEGYGPLSPLV 200
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 110 bits (264), Expect = 2e-25
Identities = 61/170 (35%), Positives = 98/170 (57%), Gaps = 1/170 (0%)
Frame = +2
Query: 200 AVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAIANGLVTREELFVTTKLWNDKHARDQVV 379
+VY A++ GYRHID+A +Y +E G+ I + + RE+++ T+KL ND +
Sbjct: 32 SVYAALQCGYRHIDSAQMYHNEADCGRAILKFMEETGTKREDIWFTSKL-NDLSGYKSTL 90
Query: 380 PALQESLKKLGLDYIDLYLIHFPIATKPDDSPDNIDYLETWQGMQDARQLGLARSIGVSN 559
++ S+K GL YIDL+L+H P D +E+W+ ++ + G R+IGVSN
Sbjct: 91 SSIDASVKACGLGYIDLFLLHSPYG----------DRIESWKALEKGVEEGKLRAIGVSN 140
Query: 560 FNATQITRLV-SNSYIRPVINQIEVNPTNTQEPLXAHCXSLGIAVMAYSP 706
F I L+ S+ I P +NQIE++P +Q+ + +C S GI + AY+P
Sbjct: 141 FGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVVDYCESKGIQLAAYAP 190
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 103 bits (246), Expect = 4e-23
Identities = 70/206 (33%), Positives = 107/206 (51%), Gaps = 1/206 (0%)
Frame = +2
Query: 92 QAPCIQLHAGXPLPXVALGTGRGTAKXSDSIXXVRQAVYWAIEAGYRHIDTAAVYQDEEQ 271
++ + L G +P + G G K ++ V QA +++GYRHIDTAAVY +E+
Sbjct: 7 ESTTVTLTNGMVIPRI--GFGAFMLKYNECYGLVTQA----LDSGYRHIDTAAVYGNEDI 60
Query: 272 VGQGIAEAIANGLVTREELFVTTKLWNDKHARDQVVPALQESLKKLGLDYIDLYLIHFPI 451
G+ I + V R ++F+T+KL N A++ SL LG YIDL+LI P
Sbjct: 61 CGKAIVDWCEKNNVKRTDIFLTSKLANCSDYY-STRAAIRSSLHHLG-TYIDLFLIQSPA 118
Query: 452 ATKPDDSPDNIDYLETWQGMQDARQLGLARSIGVSNFNATQITRL-VSNSYIRPVINQIE 628
K + +W+ M++ G RS+GVSN+ + L SN P +NQIE
Sbjct: 119 GGKK-------SRIASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIE 171
Query: 629 VNPTNTQEPLXAHCXSLGIAVMAYSP 706
++P +Q+ + +C S IA+ AYSP
Sbjct: 172 LHPFLSQDDIVKYCQSHDIAIEAYSP 197
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 83.0 bits (196), Expect = 4e-17
Identities = 49/171 (28%), Positives = 90/171 (52%), Gaps = 1/171 (0%)
Frame = +2
Query: 197 QAVYWAIEAGYRHIDTAAVYQDEEQVGQGIAEAIANGL-VTREELFVTTKLWNDKHARDQ 373
Q V A+ G R ID+A Y++E++ Q I + + + RE++ + TK+ + ++
Sbjct: 28 QQVIEALSLGIRVIDSAITYRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQGFER 87
Query: 374 VVPALQESLKKLGLDYIDLYLIHFPIATKPDDSPDNIDYLETWQGMQDARQLGLARSIGV 553
A+++SL++ G +D+ LIH P P + +E+W+ + ++ G IGV
Sbjct: 88 TWKAVEQSLRRTGRPKLDVVLIHSP------KWP--VRRIESWRALLQHQKEGRINKIGV 139
Query: 554 SNFNATQITRLVSNSYIRPVINQIEVNPTNTQEPLXAHCXSLGIAVMAYSP 706
SN+N + ++S P INQ+E + N + ++C + GI V A+SP
Sbjct: 140 SNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYCFNHGILVQAFSP 190
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 39.5 bits (88), Expect = 5e-04
Identities = 39/147 (26%), Positives = 64/147 (43%), Gaps = 15/147 (10%)
Frame = +2
Query: 317 REELFVTTKLWNDKHAR----------DQVVPALQESLKKLGLDYIDLYLIHFPIATKPD 466
R+E+F+ TK +K+ D + AL SLK+LG+D IDLY +H P
Sbjct: 75 RKEIFLATKFGYEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYVHRFSGETPI 134
Query: 467 DSPDNIDYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEVNP--T 640
+ + ++ + G R IG+S +A I R + + V Q+E +P
Sbjct: 135 E--------KIMGALKKCVEAGKIRYIGLSECSANTIRRAAAVYPVSAV--QVEYSPFSL 184
Query: 641 NTQEP---LXAHCXSLGIAVMAYSPFG 712
+ P + C I ++ Y+P G
Sbjct: 185 EIERPEIGVMKACRENNITIVCYAPLG 211
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 38.7 bits (86), Expect = 9e-04
Identities = 44/153 (28%), Positives = 70/153 (45%), Gaps = 12/153 (7%)
Frame = +2
Query: 212 AIEAGYRHIDTAAVYQD---EEQVGQGIAEA---IANGLVTREELF-VTTKLWNDKH-AR 367
A + G DTA +Y + E +G+ I E + ++T + F TKL N +R
Sbjct: 53 AWDLGINTFDTAEIYSNGNSETVMGKAIKELGWDRSEYVITTKVFFGAGTKLPNTTGLSR 112
Query: 368 DQVVPALQESLKKLGLDYIDLYLIHFPIATKPDDSPDNIDYLETWQGMQDARQLGLARSI 547
++ L SLK+LGL Y+D+ + H +PD S + E + Q G A
Sbjct: 113 KHIIEGLNASLKRLGLPYVDVIMAH-----RPDPS---VPMEEVVRAFTQLIQDGKAFYW 164
Query: 548 GVSNFNATQITRL--VSNSY--IRPVINQIEVN 634
G S ++A +I ++ Y I PV +Q + N
Sbjct: 165 GTSEWSAFEIEHAHHIATKYNLIAPVADQPQYN 197
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 32.3 bits (70), Expect = 0.074
Identities = 13/48 (27%), Positives = 28/48 (58%)
Frame = +2
Query: 488 YLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEV 631
+++TW+ +++ G ++GVS F ++ RL+S+ + P QI +
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQINI 205
>SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 708
Score = 27.5 bits (58), Expect = 2.1
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Frame = -3
Query: 640 CRIHLNLVNNRPDVTVAD---QSRDLRSVEVGY-SNGP 539
CR NL+N P++TVA ++ DL+ V SNGP
Sbjct: 443 CRFRRNLINLLPEITVAHFEAEALDLKFVAKSLPSNGP 480
>SPBC215.11c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 27.1 bits (57), Expect = 2.8
Identities = 27/105 (25%), Positives = 44/105 (41%), Gaps = 1/105 (0%)
Frame = +2
Query: 395 SLKKLGLDYIDLYLIHFPIATKPDDSPDNIDYLETWQGMQDARQLGLARSIGVSNFNATQ 574
S+++LG+ IDL+ +H + D D M ++ GL R +G+S
Sbjct: 122 SMRRLGVKQIDLWQLH-----RIDPKVPRKDQFSEIAAM---KKEGLIRHVGLSEVTVDD 173
Query: 575 ITRLVSNSYIRPVINQIEVNPTNTQ-EPLXAHCXSLGIAVMAYSP 706
I + Y V Q N N + E + +C GIA + + P
Sbjct: 174 IKE--AEQYFPVVSVQNLFNLVNRKNEKVLEYCEQKGIAFIPWYP 216
>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 521
Score = 26.2 bits (55), Expect = 4.9
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +2
Query: 446 PIATKPDDSPDNIDYLETWQGMQDARQL 529
P+AT D + ++D L T QG+ D R L
Sbjct: 428 PMATHVDQTEHDLDILVTEQGLADLRGL 455
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 26.2 bits (55), Expect = 4.9
Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 2/25 (8%)
Frame = -3
Query: 70 QTCEGDXEQANW--PDCRACGRCNT 2
Q C+ D E ANW + + C +C+T
Sbjct: 272 QKCQDDSETANWIHANTKECPKCST 296
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,950,319
Number of Sequences: 5004
Number of extensions: 55128
Number of successful extensions: 152
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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